A/O工艺中污泥浓度对微生物群落结构的影响

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Abstract The effect of different sludge concentration (MLSS) on denitrification efficiency and microbial community structure in A/O process for starch industry wastewater treatment were investigated. The 16S rDNA Miseq high throughput sequencing technique was used for bacterial commnutiy analysis. The results showed that the average removal rate of ammonia nitrogen (NH₄⁺-N) were respectively 90.2% and 67.2% when the concentration of sludge were 4066mg/L and 2985mg/L. The dominant bacteria were Proteobacteria, Bacteroidetes, Chloroflexi and Firmicutes in both of the pools; while the average abundance ratio of Proteobacteria and Bacteroides were 40.65% and 30.28% in the pool No. 1,25.25% and 33.27% in the pool No. 2respectively. In the functional gene level, all of the eight categories (KEGG, Kyoto Encyclopedia of Genes and Genomes) had a same order. The content of nitrogen metabolism related genes, such as, nitrifying ferments, denitrifying enzymes and ammonifying enzymes genes were higher in the pool No. 1. In this study, the sludge concentration has a significant effect on the bacterial structure, and the high sludge concentration is beneficial to the formation of high efficiency denitrifying bacterial community.

Key words： A/O process MLSS nitrogen removal effect Miseq bacterial community structure

Received: 08 June 2017

PACS:

X703

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	Articles by authors
	LUO Xiao
	ZHENG Xiang-yang
	ZHAO Cong-cong
	ZHANG Li-guo
	ZHONG Wei-zhang

Cite this article:

LUO Xiao,ZHENG Xiang-yang,ZHAO Cong-cong等. Effects of sludge concentration on microbial community structure in A/O process[J]. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(1): 275-283.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2018/V38/I1/275

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