基于iTRAQ技术荧蒽降解菌的比较蛋白质组学分析

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Abstract Comparative proteomics analysis was performed on proteins extracted from Rhodococcus sp. BAP-1on consecutive fluoranthene exposure days by using isobaric tags for relative and absolute quantization (iTRAQ) labeling and LC-MS/MS analysis to access differentially expressed proteins. In order to reveal the mechanism of the functional regulation of proteins in the fluoranthene-degrading bacterium, iTRAQ-based clustering and bioinformatics analysis detected a total of 796 differentially expressed proteins, including 613 up-regulated proteins and 183 down-regulated proteins. There were 111shared differentially expressed proteins in all three clusters (3d/1d, 6d/1d and 8d/1d). COG, GO enrichment and pathway enrichment analysis showed that most differentially expressed proteins were involved in the processes of metabolism and energy production. Induced by fluoranthene, the key over expressed proteins in this bacterial cells were cytochrome C, ATP synthase, nucleoside diphosphate and other kinases, some binding proteins, several dehydrogenases, ribosomal proteins and chemotaxis protein; the significant down-regulated proteins was 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase. These all regulated proteins together formed protein interaction network to regulate a series of life activities of fluoranthene-degrading bacteria.

Key words： iTRAQ comparative proteomic functional regulation of proteins PAHs bioremediation

Received: 10 June 2017

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X172

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	XU Jie
	WANG Hong-qi
	KONG De-kang

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XU Jie,WANG Hong-qi,KONG De-kang. iTRAQ-based comparative proteomic analysis of a fluoranthene-degrading bacterium[J]. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(1): 284-292.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2018/V38/I1/284

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