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The competitive relationships of PAOs-GAOs in simultaneous partial nitrification-endogenous denitrification and phosphorous removal (SPNED-PR) systems and their nutrient removal characteristics |
WANG Xiao-xia1,2, WANG Shu-ying1, ZHAO Ji1, DAI Xian1, PENG Yong-zhen1 |
1. National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China;
2. Department of Environmental Engineering, Qingdao University, Qingdao 266071, China |
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Abstract In order to analyze the nitrogen (N) and phosphorus (P) removal characteristics of simultaneous partial nitrification-endogenous denitrification and phosphorus removal (SPNED-PR) systems and to elucidate the contribution and competitive relationships between phosphorus and glycogen accumulating organisms (PAOs and GAOs) in the nutrient removal, an extended anaerobic (150min)/low aerobic (180min, dissolved oxygen (DO) concentration for 0.5~0.7mg/L) operated sequencing batch reactor (SBR) fed with domestic wastewater (C/N:around 4) was studied by investigating the effects of different DO (0.5~2.0mg/L), nitrite (4.7~39.9mg/L) and nitrate (5.0~40.0mg/L) concentrations on the nutrient removal and intracellular carbons transformation. Results showed that DO had barely effects on the aerobic metabolisms of both PAOs and GAOs, and almost no PAOs-GAOs competition was detected at various DO concentrations. GAOs had a competitive advantage over PAOs at the presence of nitrite, and nitrite was mainly removed by GAOs (about 58%); GAOs had a greater tolerance to nitrite than PAOs, which alleviated the nitrite inhibition on PAOs at high nitrite concentrations (26.2~39.9mg/L) and assured the nutrient removal in the SPNED-PR system. PAOs had a competitive advantage over GAOs when nitrate was present, and it contributed to about 61.2% of total nitrate removal. Additionally, PAOs preferred to utilize DO over nitrite and nitrate for P uptake (PURDO > PURnitrate > PURnitrite), which assured the efficient P removal at low aerobic conditions. Highly active GAOs ensured the efficient N removal in the SPNED-PR system via endogenous nitrite denitrification.
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Received: 20 July 2017
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Cite this article: |
WANG Xiao-xia,WANG Shu-ying,ZHAO Ji等. The competitive relationships of PAOs-GAOs in simultaneous partial nitrification-endogenous denitrification and phosphorous removal (SPNED-PR) systems and their nutrient removal characteristics[J]. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(2): 551-559.
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URL: |
http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2018/V38/I2/551 |
[1] |
Ma Y, Peng Y Z, Wang X L. Improving nutrient removal of the AAO process by an influent bypass flow by denitrifying phosphorus removal[J]. Desalination, 2009,246(1-3):534-544.
|
[2] |
刘青松,彭永臻,候锋,等.双污泥反硝化除磷系统中氨氮容积负荷的优化[J]. 农业工程学报, 2013,29(21):194-200.
|
[3] |
戴娴,王晓霞,彭永臻,等.进水C/N对富集聚磷菌的SNDPR系统脱氮除磷的影响[J]. 中国环境科学, 2015,35(9):2636-2643.
|
[4] |
Wang X X, Wang S Y, Zhao J, et al. A novel stoichiometries methodology to quantify functional microorganisms in simultaneous nitrification-endogenous denitrification and phosphorous removal (SNEDPR)[J]. Water Research, 2015,95:319-329.
|
[5] |
戴娴,彭永臻,王晓霞,等.不同厌氧时间对富集聚磷菌的SNDPR系统处理性能的影响[J]. 中国环境科学, 2016,36(1):92-99.
|
[6] |
王晓霞,王淑莹,赵骥,等.厌氧/好氧SNEDPR系统处理低C/N比污水的优化运行[J]. 中国环境科学, 2016,36(9):2672-2680.
|
[7] |
Lo I W, Lo K V, Mavinic D S, et al. Contributions of biofilm and suspended sludge to nitrogen transformation and nitrous oxide emission in hybrid sequencing batch system[J]. Journal of Environmental Science, 2010,22(7):953-960.
|
[8] |
Coma M, Verawaty M, Pijuan M, et al. Enhancing aerobic granulation for biological nutrient removal from domestic wastewater[J]. Bioresource Technology, 2012,103:101-108.
|
[9] |
Gieseke A, Arnz P, Amann R, et al. Simultaneous P and N removal in a sequencing batch biofilm reactor:insights from reactor and microscale investigations[J]. Water Research, 2002, 36:501-509.
|
[10] |
Amann R I, Krumholz L, Stahl D A. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology[J]. Journal of Bacteriology, 1990,172(2):762-770.
|
[11] |
Wachtmeister A, Kuba T, Van Loosdrecht M C M, et al. A sludge characterization assay of aerobic and denitrifying phosphorus removing sludge[J]. Water Research, 1997,31(3):471-478.
|
[12] |
Grocetti G R, Hugenholts P, Bond P L. Identification of polyphosphate accumulating organisms and design of 16SrRNA-directed probes for their detection and quantification[J]. Applied and Environmental Microbiology, 2000,66(3):1175-1182.
|
[13] |
国家环境保护总局.水和废水监测分析方法[M]. 北京:中国环境科学出版社, 2002:252-354.
|
[14] |
Coma M, Puig S, Balaguer M D, et al. The role of nitrate and nitrite in a granular sludge process treating low-strength wastewater[J]. Chemical Engineering Journal, 2010,164(1):208-213.
|
[15] |
Kerrnjespersen J, Henze M. biological phosphorus uptake under anoxic and aerobic conditions[J]. Water Research, 1993,27(4):617-624.
|
[16] |
Smolders G, Vandermeij J, Vanloosdrecht M, et al. Stoichiometric model of the aerobic metabolism of the biological phosphorus removal process[J]. Biotechnology and Bioengineering, 1994, 44(7):837-848.
|
[17] |
Meinhold J, Arnold E, Isaacs S. Effect of nitrite on anoxic phosphate uptake in biological phosphorus removal activated sludge[J]. Water Research, 1999,33(8):1871-1883.
|
[18] |
史静,吕锡武,朱光灿.好氧聚磷污泥反硝化除磷特性研究[J]. 水处理技术, 2012,38(5):36-39.
|
[19] |
Hu J Y, Ong S L, Ng W J, et al. A new method for characterizing denitrifying phosphorus removal bacteria by using three different types of electron acceptors[J]. Water Research, 2003,37(14):3463-3471.
|
[20] |
王亚宜,王淑莹,彭永臻. MLSS, pH及NO2--N对反硝化除磷的影响[J]. 中国给水排水, 2005,21(7):47-51.
|
[21] |
Guisasola A, Qurie M, Del marvargas M, et al. Failure of an enriched nitrite-dpao population to use nitrate as an Electron Acceptor[J]. Process Biochemistry, 2009,44(7):689-695.
|
[22] |
Hou H, Wang S, Peng Y, et al. Anoxic phosphorus removal in a pilot scale anaerobic-anoxic oxidation ditch process[J]. Frontiers of Environmental Science & Engineering in China, 2009, 3(1):106-111.
|
[23] |
Pijuan M, Ye L, Yuan Z. Free nitrous acid inhibition on the aerobic metabolism of poly-phosphate accumulating organisms[J]. Water Research, 2010,44(20):6063-6072.
|
[24] |
杨杰,李冬,罗亚红,等.SBR后置缺氧反硝化除磷的启动及去除性能[J]. 中国环境科学, 2016,36(5):1376-1383.
|
|
|
|