|
|
Isolation and identification of quizalofop-p-ethyl-degrading strain Bacillus subtilis H and its degradation characteristics |
HOU Ying1,2, LI Jing-quan1, YOU Xiao-yan2, WANG Wei-yu2, PEI Tao2, SUN Jun-jie2 |
1. Inner Mongolia Key Laboratory of Environmental Pollution Control & Waste Resource, School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China;
2. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China |
|
|
Abstract Strain H degrading quizalofop-p-ethyl (QE) was isolated by using QE as sole carbon source from the soil contaminated by QE for a long time. Strain H was identified as Bacillus subtilis based on morphological properties, physiological and biochemical characteristics and 16S rRNA gene analysis. The degradation rate of 100mg/L QE was over 95% within 72h when strain H was cultured at temperature of 30~42℃ and pH 7~9. The degradation product of QE was identified as quizalofop acid(QA) by UPLC-MS. This is the first report of quizalofop-p-ethyl degrading by Bacillus subtilis.
|
Received: 08 September 2017
|
|
|
|
|
[1] |
Mantzos N, Karakitsou A, Nikolaki S, et al. Dissipation and transport of quizalofop-p-ethyl herbicide in sunflower cultivation under field conditions[J]. Environmental Science and Pollution Research, 2016,23(4):3481-3490.
|
[2] |
Mustafa Y, Arikan E S. Genotoxicity testing of quizalofop-P-ethyl herbicide using the Allium cepa anaphase-telophase chromosome aberration assay[J]. Caryologia, 2008,61(1):45-52.
|
[3] |
Doganlar Z B. Quizalofop-p-ethyl-induced phytotoxicity and genotoxicity in Lemna minor and Lemna gibba[J]. Journal of Environmental Science and Health, Part A:Toxic/Hazardous Substances and Environmental Engineering, 2012,47(11):1631-1643.
|
[4] |
赵小会,程翠利,周春姣,等.10%精喹禾灵乳油对斑马鱼的急性毒性试验[J]. 广州化工, 2016,44(16):108-110.
|
[5] |
朱丽珍.两种芳氧苯氧基丙酸酯类除草剂对斑马鱼毒性效应及作用机制研究[D]. 北京:中国农业大学, 2016.
|
[6] |
Elefsiniotis I S, Liatsos G D, Stamelakis D, et al. Mixed Cholestatic/Hepatocellular liver injury induced by the herbicide quizalofop-p-ethyl[J]. Environmental Health Perspectives, 2007,115(10):1479-1481.
|
[7] |
陈日萍,陈彤,俞少勇,等.芳氧苯氧丙酸类除草剂对大鼠睾丸生精细胞的损伤作用[J]. 浙江省医学科学院学报, 2006,4:22-25.
|
[8] |
陈日萍,高明,蔡冬苗,等.精喹禾灵亚慢性经口暴露对雄性大鼠的生殖毒性研究[J]. 毒理学杂志, 2011,25(3):208-211.
|
[9] |
中国质量新闻网.加拿大确定乙基精喹禾灵等农残最大残留限量[EB/OL]. http://www.cqn.com.cn/news/zjpd/spaq/1133795.html,2016-03-22.
|
[10] |
邹荣仟,吴德杰,吴林,等.精喹禾灵乳油在越橘果实及土壤中的残留动态[J]. 吉林农业大学学报, 2009,31(5):652-655.
|
[11] |
汤富彬.精喹禾灵的土壤微生物降解[D]. 杭州:浙江大学, 2002.
|
[12] |
王晓环,王德飘,康頔,等.影响精喹禾灵在土壤中降解的因素研究[J]. 辽宁化工, 2016,(5):540-543.
|
[13] |
吕欣,彭霞薇,呼庆,等.利用DGGE-菌落原位杂交法分离土壤中精喹禾灵降解菌[J]. 环境科学, 2013,34(1):263-270.
|
[14] |
周丽兴,万树青,陈泽鹏,等.短小芽孢杆菌(Bacillus pumilis)对精喹禾灵的降解特性[J]. 农药, 2006,45(9):627-629.
|
[15] |
李梦雅,李杰,戴纯,等.一株高效精喹禾灵降解菌的筛选、鉴定及降解特性研究[J]. 基因组学与应用生物学, 2017,(2):686-692.
|
[16] |
Zhang H, Li M, Li J, et al. A key esterase required for the mineralization of quizalofop-p-ethyl by a natural consortium of Rhodococcus sp JT-3and Brevundimonas sp JT-9[J]. Journal of Hazardous Materials, 2017,327:1-10.
|
[17] |
Zhang H, Li M, Dai C, et al. Characterization of EstQE, a new member of esterase family VⅢ from the quizalofop-P-ethyl-degrading bacterium Ochrobactrum sp QE-9[J]. Journal of Molecular Catalysis B Enzymatic, 2016,133:167-175.
|
[18] |
Nie Z J, Hang B J, Cai S, et al. Degradation of cyhalofop-butyl (CyB) by Pseudomonas azotoformans strain QDZ-1, and cloning of a novel gene encoding CyB-hydrolyzing esterase[J]. Journal of Agricultural and Food Chemistry, 2011,59:6040-6046.
|
[19] |
Hou Y, Tao J, Shen W J, et al. Isolation of the fenoxaprop-ethyl (FE)-degrading bacterium Rhodococcus sp. T1, and cloning of FE hydrolase gene feh[J]. FEMS Microbiology Letters, 2011,323(2):196-203.
|
[20] |
Dong W L, Sheng J, Shi K, et al. Biodegradation of fenoxaprop-P-ethyl (FE) by Acinetobacter sp. strain DL-2and cloning of FE hydrolase gene afeH[J]. Bioresource Technology, 2015,186:114-121.
|
[21] |
Liu H M, Lou X, Ge Z J, et al. Isolation of an aryloxyphenoxy propanoate (AOPP) herbicide-degrading strain Rhodococcus ruber JPL-2and the cloning of a novel carboxylesterase gene (feh)[J]. Brazilian Journal of Microbiology, 2015,46(2):425-432.
|
[22] |
Bezza F A, Chirwa E M N. Production and applications of lipopeptide biosurfactant for bioremediation and oil recovery by Bacillus subtilis CN2[J]. Biochemical Engineering Journal, 2015,101:168-178.
|
[23] |
Sariwati A, Purnomo A S, Kamei I. Abilities of co-cultures of brown-rot fungus Fomitopsis pinicola and Bacillus subtilis on biodegradation of DDT[J]. Current Microbiology, 2017,(4):1-8.
|
[24] |
东秀珠,蔡妙英.常见细菌系统鉴定手册[M]. 北京:科学出版社, 2001.
|
[25] |
Dong W L, Hou Y, Xi X D, et al. Biodegradation of fenoxaprop-ethyl by an enriched consortium and its proposed metabolic pathway[J]. International Biodeterioration & Biodegradation, 2015,97:159-167.
|
|
|
|