抗氧化剂对二氧化硫诱导的大鼠心脏线粒体损伤的缓解作用

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Abstract

Male Wistar rats were exposed to SO₂ (7mg/m³) for 28 days, 4 hours per day in SO₂ group. Rats in SO₂+NALC group were exposed to SO₂ and NALC (50mg/kg b.w., i.p.) every other day, which was dissolved in saline. Rats in control group were exposed to filtered air and saline. The mRNA levels of complexes IV and V subunits (CO1 & ATP6) and three mitochondrial transcript factors (PGC1-α, NRF1, TFAM) were analyzed by real-time RT-PCR after exposure. And the protein levels of the above three mitochondrial transcript factors were detected by Western blot. The results showed that the mRNA and protein expression levels of PGC1-α, NRF1and TFAM were decreased significantly after SO₂ inhalation, combined with the down-regulations of CO1 & ATP6 on mRNA levels. But NALC could alleviate the depressions of these genes. It indicated that SO₂ exposure could impact the transcription of mitochondria DNA through PGC1-α-NRF1-TFAM down-regulation, interfere the synthesis of important components of oxidative phosphorylation. The mechanism might be related to the production of free radicals. Antioxidants could be used to alleviate sulfur dioxide-induced mitochondrial damage in rat hearts.

Key words： SO₂ antioxidants mitochondria oxidative phosphorylation

Received: 06 January 2018

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	XIA Jin
	QIN Guo-hua
	SANG Nan

Cite this article:

XIA Jin,QIN Guo-hua,SANG Nan. Antioxidants alleviated sulfur dioxide-induced mitochondrial damage in rat hearts[J]. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(8): 3129-3134.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2018/V38/I8/3129

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