壬基酚聚氧乙烯醚对成年雄性斑马鱼HPG轴相关基因表达的影响

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Abstract

In the present study, the semi-static exposure test method was used for elucidating the effects of different concentrations of nonylphenol polyethylene glycol ether (NPEO) on the hypothalamic-pituitary-gonadal axis (HPG axis) of adult male zebrafish (Danio rerio). The results showed that the expressions of GnRH2, GnRHR1, GnRHR2, GnRHR4, FSHβ and LHβ genes in the brain, as well as the expression of LHR gene in the testis of zebrafish were significantly up-regulated under the exposure of NPEO at the experimental concentrations. The expressions of GnRHR and LHR genes were sensitively to the NPEO exposure at relatively low concentrations and the most sensitive biomarkers were the expressions of GnRHR4 and LHR genes, which were significantly induced under the exposure to 0.001mg/L of NPEO. The expression of CYP17 gene was significantly inhibited in the testis of fish exposed to NPEO at 0.1 and 10mg/L NPEO and on the contrary, the expression of CYP19a gene was significantly induced in the 10mg/L of NPEO group. Up-regulation of GnRH gene indicated that the exposure to NPEO could induce the secretion of GnRH in the hypothalamus and stimulate the secretion of GtH in the pituitary of zebrafish in turn. The exposure to NPEO could promote the synthesis of endogenous estrogen in zebrafish by up-regulating the expression of CYP19a gene and inhibit the synthesis of testosterone (T) by down-regulating the expression of CYP17 gene, and therefore disrupt the sexual hormone homeostasis in zebrafish. The increased levels of estrogen in the testis of zebrafish could produce the negative feedback to the pituitary gland and then stimulated the secretion of GtH in the pituitary, suggesting that NPEO at the experimental concentrations (0.001~10mg/L) could affect the feedback regulation of the HPG axis in male adult zebrafish.

Key words： nonylphenol ethoxylate male zebrafish endocrine disrupting effects HPG axis

Received: 05 January 2018

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	Articles by authors
	JIANG Jin-lin
	LIU Ren-bin
	ZHANG Yu-feng
	LIANG Xia
	SHAN Zheng-jun

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JIANG Jin-lin,LIU Ren-bin,ZHANG Yu-feng等. Effects of nonylphenol ethoxylate exposure on genes expression along HPG axis of adult male zebrafish[J]. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(8): 3135-3142.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2018/V38/I8/3135

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