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| Quantitative source analysis of microorganism pollution in the surface water in Xijiang River Basin |
| ZHANG Yang1,2, WU Ren-ren2,3, YANG Ge4, WANG Guang2,3, WANG Yi-shu2,3, LIN Kai-rong1, LI Kai-ming2,3, ZHONG Jie5 |
1. Department of Water Resources and Environment, Sun Yat-sen University, Guangzhou 510275, China;
2. South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangzhou 510530, China;
3. The key Laboratory of Water and Air Pollution Control of Guangdong Province, Guangzhou 510530, China;
4. Zhaoqing environmental protection monitoring station, Zhaoqing 526040, China;
5. Department of Environmental Science and Engineering, Zhongkai University of Agriculture and Technology, Guangzhou 510550, China |
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Abstract Understanding the characteristic of source strength of the microbial gene marker is the key for quantitative analyzing the microbial contamination from different sources. In this study, the source strength of human-(qHS601F/qBac725R), porcine-(Bac41F/Bac163R), cattle-(BacB2-590F/Bac708Rm) and poultry-specific(qC160F-HU/qBac265R-HU) bacteroides primers were explored by qPCR method, and then quantitative analysis of the source and level of microbial pollution in Xijiang river in Guangdong province. The results showed that the source strength of different specific bacteroides primers was human(qHB) > porcine(qPB) > cattle(qRB) > poultry(qCB). The concentration of DNA had little effect on source strength of each primers. The selected target area was generally contaminated by human, pig, cattle and poultry feces. The concentration of human, porcine and poultry-specific primers were increased slowly from upstream to downstream in the main stream. In the tributary, the mean concentration of human-, porcine-and poultry-specific primers were increased 1-order magnitude compared to the main stream. qRB had little change in different section of the river. This suggests that the pollution of domestic sewage, porcine source and poultry excrement in the downstream was more serious. The influence of cattle source pollution was not significant. Statistic analysis showed that human-specific premier had a significant correlation with traditional fecal indicator bacteria in the tributary which had high level of fecal pollution(FIB)(P<0.05). It reflected that human source was the major reason for the high concentration of FIB, and human feces was the persistent pollution source.
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Received: 27 March 2018
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