海河天津段微囊藻及其毒素的空间分布及与水环境因子的关系

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Abstract

The spatial distribution of Microcystis and microcystin in Haihe River during water bloom were investigated using quantitative real-time PCR (qPCR) and ELISA techniques. Microcystin synthetase gene (mcyD) and PC-IGS fragments were used as the target the total microcystin population and the potential microcystin-producing subpopulation. The results showed that microcystin existed as a mixed population of potentially toxic and non-toxic genotypes in Haihe River. There was significantly spatial variation in the abundance of microcystin-producing Microcystis. The abundance of microcystin-producing Microcystis subpopulation was ranged from 1.16×10⁴ to 2.48×10⁷ copies/mL and the ratio of its abundance to total microcystin varied from 4.25% to 28.59%. The peak value of microcystin was site point 8, which 1mL water contained 195.51μg microcystin in Microcystis algae cells and 0.97μg/L in water. To sum up, total abundance of Microcystis remained a high level in Haihe Tianjin region. The toxin of Microcystis showed various among different sampling sites. The most significant impact factors for Microcystis' abundance was pH value. However, the content of microcystin is not upon the value of WHO's threshold. But the intercellular toxin in a given volume of water remains a high level, which may cause the potential risk to the ecosystem of down flow estuary.

Key words： Microcystis microcystin quantitative real-time PCR ELISA techniques Haihe River

Received: 23 March 2018

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	Articles by authors
	HUO Da
	CAO Qi
	WANG Su-yan
	CHEN Pei-pei
	LI Yi-ming
	QIAO Zhi-yi

Cite this article:

HUO Da,CAO Qi,WANG Su-yan等. The spatial distribution of Microcystis and microcystin and its relationship with environmental factors in Haihe Tianjin City[J]. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(10): 3897-3903.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2018/V38/I10/3897

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