|
|
|
| Variations of Antibiotic resistance genes during lincomycin mycelia dreg composting |
| REN Sheng-tao1, GUO Xia-li1, LU A-qian1, ZHANG Qian-qian1, GUO Xiao-ying1, WANG Yan1, WANG Lian-zhong2, ZHANG Bao-bao2 |
1. College of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou 450001, China;
2. Henan Xinxiang Hua Xing Pharmaceutical Factory, Xinxiang 453731, China |
|
|
|
|
Abstract In order to investigate the variation of antibiotic resistance genes during the antibiotic mycelia dregs composting process, five antibiotic resistance genes (lnuA-01, sul1, ermA, ermB, ermC) and one horizontal gene transfer (intI1) were detected from the compost by quantitative PCR technique. The results showed that 99% lincomycin was degraded after composting. For both the lincomycin mycelia dreg compost and sewage sludge compost, the absolute total of ARGs was all increased. By contrast, the relative total of ARGs were all decreased by 5% and 22% respectively. IntI1 was enriched in lincomycin mycelia dreg compost indicating the potential ecological risk. The redundancy analysis revealed that the antibiotic resistance genes were greatly influenced by the environmental factors and the order was pH > licomycin residue > temperature > C/N.
|
|
Received: 13 April 2018
|
|
|
|
|
|
| [1] |
Zhu Y, Gillings M, Simonet P, et al. Microbial mass movements[J]. Science, 2017,357(6356):1099-1100.
|
| [2] |
苏建强,黄福义,朱永官.环境抗生素抗性基因研究进展[J]. 生物多样性, 2013,21(4):481-487.
|
| [3] |
周启星,罗义.抗生素抗性基因(ARGs) -一种新型环境污染物[J]. 环境科学学报, 2008,28(8):1499-1505.
|
| [4] |
Duan M, Gu J, Wang X, et al. Effects of genetically modified cotton stalks on antibiotic resistance genes, intI1, and intI2 during pig manure composting[J]. Ecotoxicology and Environmental Safety, 2018,147:637-642.
|
| [5] |
Zhu Y, Johnson T A, Su J, et al. Diverse and abundant antibiotic resistance genes in Chinese swine farms[J]. Proceedings of the National Academy of Sciences, 2013,110(9):3435-3440.
|
| [6] |
He L, Liu Y, Su H, et al. Dissemination of Antibiotic Resistance Genes in Representative Broiler Feedlots Environments:Identification of Indicator ARGs and Correlations with Environmental Variables[J]. Environmental Science & Technology, 2014,48(22):13120-13129.
|
| [7] |
Zhang J, Chen M, Sui Q, et al. Impacts of addition of natural zeolite or a nitrification inhibitor on antibiotic resistance genes during sludge composting[J]. Water Research, 2016,91:339-349.
|
| [8] |
张宁,李淼,刘翔.土壤中抗生素抗性基因的分布及迁移转化[J]. 中国环境科学, 2018,38(7):2609-2617.
|
| [9] |
D'Costa V M, King C E, Kalan L, et al. Antibiotic resistance is ancient[J]. Nature, 2011,477(7365):457-461.
|
| [10] |
黄福义,安新丽,李力,等.生活垃圾填埋场对河流抗生素抗性基因的影响[J]. 中国环境科学, 2017,37(1):203-209.
|
| [11] |
彭双,王一明,林先贵.连续施用发酵猪粪对土壤中四环素抗性基因数量的影响[J]. 中国环境科学, 2015,35(4):1173-1180.
|
| [12] |
成玉婷,吴小莲,向垒,等.广州市典型有机蔬菜基地土壤中磺胺类抗生素污染特征及风险评价[J]. 中国环境科学, 2017,37(3):1154-1161.
|
| [13] |
Tien Y, Li B, Zhang T, et al. Impact of dairy manure pre-application treatment on manure composition, soil dynamics of antibiotic resistance genes, and abundance of antibiotic-resistance genes on vegetables at harvest[J]. Science of the Total Environment, 2017, 581-582:32-39.
|
| [14] |
Zhang Q, Ying G, Pan C, et al. Comprehensive Evaluation of Antibiotics Emission and Fate in the River Basins of China:Source Analysis, Multimedia Modeling, and Linkage to Bacterial Resistance[J]. Environmental Science & Technology, 2015,49(11):6772-6782.
|
| [15] |
李再兴,田宝阔,左剑恶,等.抗生素菌渣处理处置技术进展[J]. 环境工程, 2012,30(2):72-75.
|
| [16] |
张红娟,郭夏丽,王岩.林可霉素菌渣与牛粪联合堆肥实验研究[J]. 环境工程学报, 2011,5(1):231-234.
|
| [17] |
温沁雪,陈希,张诗华,等.城市污泥混合青霉素菌渣堆肥实验[J]. 哈尔滨工业大学学报, 2014,46(4):43-49.
|
| [18] |
Ren S, Guo X, Lu A, et al. Effects of co-composting of lincomycin mycelia dregs with furfural slag on lincomycin degradation, maturity and microbial communities[J]. Bioresource Technology, 2018,265:155-162.
|
| [19] |
Zhang Z, Zhang D, Zhao J, et al. Evaluation of aerobic co-composting of penicillin fermentation fungi residue with pig manure on penicillin degradation, microbial population dynamics and composting maturity[J]. Bioresource Technology, 2015,198:403-409.
|
| [20] |
Chen Z, Zhang S, Wen Q, et al. Effect of aeration rate on composting of penicillin mycelial dreg[J]. Journal of Environmental Sciences, 2015,37:172-178.
|
| [21] |
Fu L, Huang T, Wang S, et al. Toxicity of 13different antibiotics towards freshwater green algae Pseudokirchneriella subcapitata and their modes of action[J]. Chemosphere, 2017,168:217-222.
|
| [22] |
Liu N, Han H, Yin H, et al. Variations in the fate and risk analysis of amoxicillin and its degradation products during pig manure aerobic composting[J]. Journal of Hazardous Materials, 2018,346:234-241.
|
| [23] |
Li H, Duan M, Gu J, et al. Effects of bamboo charcoal on antibiotic resistance genes during chicken manure composting[J]. Ecotoxicology and Environmental Safety, 2017,140:1-6.
|
| [24] |
Qian X, Sun W, Gu J, et al. Reducing antibiotic resistance genes, integrons, and pathogens in dairy manure by continuous thermophilic composting[J]. Bioresource Technology, 2016,220:425-432.
|
| [25] |
段会英,赵娟,张振华,等.青霉素菌渣堆肥中β-内酰胺酶基因丰度变化[J]. 中国环境科学, 2017,37(10):3873-3881.
|
| [26] |
Yang L, Zhang S, Chen Z, et al. Maturity and security assessment of pilot-scale aerobic co-composting of penicillin fermentation dregs (PFDs) with sewage sludge[J]. Bioresource Technology, 2016,204:185-191.
|
| [27] |
Li L, Sun J, L B, et al. Quantification of lincomycin resistance genes associated with lincomycin residues in waters and soils adjacent to representative swine farms in China[J]. Frontiers in Microbiology, 2013,4:364.
|
| [28] |
Song W, Wang X, Zhang S, et al. Effects of different swine manure to wheat straw ratios on antibiotic resistance genes and the microbial community structure during anaerobic digestion[J]. Bioresource Technology, 2017,231:1-8.
|
| [29] |
Ezzariai A, Barret M, Merlina G, et al. Evaluation of the antibiotics effects on the physical and chemical parameters during the co-composting of sewage sludge with palm wastes in a bioreactor[J]. Waste Management. 2017,68:388-397.
|
| [30] |
任省涛,郭夏丽,芦阿虔,等.林可霉素菌渣堆肥微生物群落多样性分析[J]. 环境科学, 2018,39(10):4817-4824.
|
| [31] |
Qian X, Sun W, Gu J, et al. Variable effects of oxytetracycline on antibiotic resistance gene abundance and the bacterial community during aerobic composting of cow manure[J]. Journal of Hazardous Materials, 2016,315:61-69.
|
| [32] |
Miller J, Novak J, Knocke W, et al. Elevation of antibiotic resistance genes at cold temperatures:implications for winter storage of sludge and biosolids[J]. Letters in Applied Microbiology, 2014,59(6):587-593.
|
| [33] |
Su J, Wei B, Ou-Yang W, et al. Antibiotic Resistome and Its Association with Bacterial Communities during Sewage Sludge Composting[J]. Environmental Science & Technology, 2015,49(12):7356-7363.
|
| [34] |
Chen Y, Jiang Y, Huang H, et al. Long-term and high-concentration heavy-metal contamination strongly influences the microbiome and functional genes in Yellow River sediments[J]. Science of the Total Environment, 2018,637-638:1400-1412.
|
|
|
|
