高效铜绿微囊藻溶藻菌WJ6的分离鉴定及溶藻特性

Abstract
Figure/Table
References (25)
Related Citation (15)

Download: PDF (395 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract

An algicidal bacteria strain with a strong algicidal effect to M.aeruginosa was isolated from the eutrophic water. Then, the algicidal bacteria was identified as WJ6 by analyzing it's morphological features, physiological and biochemical characteristics, as well as phylogenetic analysis of 16S rDNA sequences. The algae-lysing means of WJ6 and the effects of different culture periods, concentrations of bacterium and environmental factors on the algae-lysing action were investigated to analyze the algicidal characteristics and possible algae-lysing mechanism of the strain WJ6. And the results showed that WJ6 was identified as Serratia sp.(Accession No.KY462187). The algicidal mechanism of WJ6 is indirectively, which disolves the algae mainly by an extracellular products. At logarithmic growth phase, the strain WJ6 can dissolve M. aeruginosa with a removal rate of 87.50%. The bacteria WJ6with on centration more than 1.4×10⁹CFU/mL can give the highest removal rate of 95.69%. Under the condition of 30℃ and pH8, the removal rate is 90.00%. The effect of modified basic mediumon the algae-lysing effect of WJ6 is the most obvious with the higests removal rate of 98.50%. Furthermore, the removal rate of the algicidal bacterium is 98.33% when the ammonium ion concentration in the mediumis above 2mol/L. The removal rate is 92.77% when the salinity is 0.5%. All the results stated above indicate that the algicidal bacteria strain WJ6 have a strong algicidal effect to M. aeruginosa and prospective application in the control of M. aeruginosa in eutrophic water.

Key words： algicidal bacteria isolation and identification Microcystis aeruginosa algicidal characteristics

Received: 03 April 2018

:	X172
	X524

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	HONG Gui-yun
	MA Shao-xiong
	WANG Jia
	ZHANG Jin

Cite this article:

HONG Gui-yun,MA Shao-xiong,WANG Jia等. Isolation and identification of an efficient algicidal bacteria strain and algicidal characteristics on Microcystis aeruginosa[J]. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(11): 4269-4275.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2018/V38/I11/4269

[1]	Oh M Y, Lee B S, Jin D H, et al. Isolation of algicidal compounds from the red alga Corallina pilulifera against red tide microalgae[J]. Journal of Applied Phycology, 2010,22(4):453-458.
[2]	李大命,周军,唐晟凯,等.河蟹养殖池塘微囊藻水华毒性及其光合作用活性特征[J]. 湖泊科学, 2015,27(1):113-118.
[3]	Alexova R, Fujii M, Birch D. Iron uptake and toxin synthesis in the bloom forming Microcystis aeruginosa under iron limitation[J]. Environmental Microbiology, 2011,13(4):1064-1077.
[4]	张世羊,成水平,贺锋,等.两种不同株铜绿微囊藻培养液对大型溞的毒性效应研究[J]. 水生生物学报, 2008,32(5):637-642.
[5]	Imai I, Kimura S. Resistance of the fish-killing dinoflagllate Cochlodinium polykrikoides against algicidal bacteria isolated from the coastal sea of Japan[J]. Harmful Algae, 2008,7:360-367.
[6]	Sherman L A, Jr R M B. Cyanophages and Viruses of Eukaryotic Algae[M]. Newly Characterized Protist and Invertebrate Viruses. Springer US, 1978:145-234.
[7]	Baker K H, Herson D S. Interactions between the diatom Thallasiosira pseudonanna and an associated pseudomonad in a mariculture system[J]. Applied & Environmental Microbiology, 1978,35(4):791-796.
[8]	汪辉,刘玲,牛丹丹,等.一株海洋细菌对中肋骨条藻的溶解效应及其溶藻特性[J]. 中国环境科学, 2011,31(6):971-977.
[9]	Proctor L M, Fuhrman J A. Viral mortality of marine bacteria and cyanobacteria[J]. Nature, 1990,343(6253):60-62.
[10]	Imai I, Ishida Y, Hata Y. Killing of marine phytoplankton by a gliding bacterium Cytophaga sp. isolated from the coastal sea of Japan[J]. Marine Biology, 1993,116(4):527-532.
[11]	Banin E, Khare S K, Naider F. Proline-rich peptide from the coral pathogen Vibrio shiloi that inhibits photosynthesis of Zooxanthellae[J]. Applied & Environmental Microbiology, 2001,67(4):1536-1541.
[12]	Furusawa G, Yoshikawa T, Yasuda A. Algicidal activity and gliding motility of Saprospira sp. SS98-5[J]. Canadian Journal of Microbiology, 2003,49(2):92-100.
[13]	Luo J, Wang Y, Tang S. Isolation and Identification of Algicidal Compound from Streptomyces and Algicidal Mechanism to Microcystis aeruginosa[J]. Plos One, 2013,8(10):e76444.
[14]	马宏瑞,章欣,王晓蓉,等.芽孢杆菌Z5溶铜绿微囊藻特性研究[J]. 中国环境科学, 2011,31(5):828-833.
[15]	李三华,张奇亚.1株溶藻菌的部分生物学特性及溶鱼腥藻作用[J]. 环境科学, 2013,34(2):583-588.
[16]	Li Y, Liu L, Xu Y T, et al. Stress of algicidal substances from a bacterium Exiguo bacterium sp. h10 on Microcystis aeruginosa[J]. Letters in Applied Microbiology, 2017,64(1):57-65.
[17]	国家环保总局.水和废水监测分析方法[M]. 第4版.北京:中国环境科学出版社, 2002:670-672.
[18]	周瑞,喻融,王梓入,等.溶藻细菌混合培养对铜绿微囊藻溶藻效果的初步研究[J]. 华中师范大学研究生学报, 2005,12(4):138-141.
[19]	廖春丽,杨闪闪,许晨,等.一株溶藻细菌NP23的初步分离鉴别及其溶藻作用研究[J]. 生物技术通报, 2012,8:163-167.
[20]	黄现恩,谷青,史全良.一株具有藻毒素降解和溶藻功能细菌的分离鉴定[J]. 环境工程学报, 2016,10(7):3919-3924.
[21]	张晖,何颖,曹驰骋,等.1株巢湖高效溶藻菌的分离鉴定及其溶藻特性研究[J]. 环境科学与技术, 2018,41(7):1-5.
[22]	程新,李昆太,黄林.一株枯草芽孢杆菌的生长特性及抑藻效果研究[J]. 生物技术通报, 2017,33(7):120-125.
[23]	张睿,王广军,李志斐,等.枯草芽孢杆菌对铜绿微囊藻抑制效果的研究[J]. 中国环境科学, 2015,35(6):1814-1821.
[24]	Su J Q, Yang X R, Zheng T L. Isolation and characterization of a marine algicidal bacterium against the toxic dinoflagellate Alexandrium tamarense[J]. Harmful Algae, 2007,6(6):799-810.
[25]	黄晶晶,汪雪婷,夏赛周,等.溶藻细菌MS7的分类鉴定及其溶藻活性的初步研究[J]. 海洋与湖沼, 2013,44(3):632-637.