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| Differential membrane protein analysis of Rhodococcus during the transmembrane-transport process of fluoranthene |
| KONG De-kang1, LI Yi2, WANG Hong-qi1, XU Jie1, GUAN Jing-jing1 |
1. College of Water Sciences, Beijing Normal University, Beijing 100875, China;
2. College of Environment and Resource, Guangxi Normal University, Guilin 541004, China |
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Abstract Comparative proteomics analysis was performed on membrane proteins extracted from Rhodococcus sp. BAP-1on consecutive fluoranthene exposure days by using isobaric tags for relative and absolute quantization (iTRAQ) labelling and LC-MS/MS analysis to access differentially expressed membrane proteins. A total of 172differential membrane proteins were identified. The enrichment of COG and GO terms analysis of differentially expressed membrane proteins in three clusters showed that most of the differential proteins were involved in transport and oxidation-reduction processes. ABC transporters and TonB-dependent receptors played an important role in transmembrane-transport of fluoranthene. Catalase and superoxide dismutase had a significant up-regulation in sixth days as an antioxidant defence mechanism to protect microbes. Various energy-produced proteins played their respective roles at different stages, and constituted a protein interaction network to regulate transmembrane-transport of microorganisms.
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Received: 16 May 2018
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