基质冲击对ANAMMOX颗粒释放AHLs及颗粒特性的影响

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Abstract Effects of substrate shock on the characteristics of ANAMMOX granules were investigated from the perspective of quorum sensing with the final purpose of providing theoretical guidance and reference for improving the stability and settleability of ANAMMOX granules under substrate shock. The results showed that due to 24h 1500mg/L total nitrogen (TN) shock, the amount of AHLs released by ANAMMOX granules increased significantly from 4.3 to 10.0. At the same time, excessive release of bound extracellular polymeric substances (B-EPS) (increased by 107.3mg/g VSS) was observed, which led to the poor settling performance of granules (density and settling velocity reduced by 53% and 33%, respectively). However, after the TN concentration returned to the level in the stable period, the release amount of AHLs, B-EPS content and granules properties could also return gradually to the level in the stable period. It was inferred that substrate shock resulted in the change of release amount of AHLs, which caused the change of B-EPS production. The results of the batch test also confirmed that the substrate concentration affected the release of AHLs, significantly. Moreover, B-EPS production was closely related to the release amount of AHLs. When the TN concentration was 1000mg/L, the release amount of AHLs was up to 11.7, leading to excessive accumulation of loosely-bound EPS (LB-EPS) (increased by 69mg/g VSS) and poor settleability of granules comparing with 200mg/L TN concentration. In contrast, the tightly-bound EPS (TB-EPS) content was not affected by substrate concentration. Thus, the LB-EPS was considered the key factor for the deterioration of granule settleability. Low concentration inhibitor can effectively inhibit the excessive release of AHLs caused by high substrate concentration, resulting in 36% reduction in LB-EPS content and improvement of the settleability and activity of granules.

Key words： ANAMMOX granules substrate shock Acyl Homoserine Lactones granules properties

Received: 09 August 2018

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X703

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	ZHANG Jing
	ZHANG Ya-chao
	WANG Xiu-jie
	LI Jun
	LIANG Dong-bo

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ZHANG Jing,ZHANG Ya-chao,WANG Xiu-jie等. Effects of substrate shock on AHL signals release of ANAMMOX granules and characteristics of granules[J]. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(3): 1009-1018.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2019/V39/I3/1009

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