低温投加短程硝化污泥下城市污水SPN/A工艺运行特性

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Abstract

In this study, feasibility of enhancing ammonia-oxidizing bacteria (AOB) activity by biological addition under the condition of temperature fluctuation and further improving the denitrification effect of Single-stage Partial Nitrification and Anammox (SPN/A) process in municipal wastewater treatment was investigated. Two sequencing batch reactors (SBR) SBR1 and SBR² were operated in intermittent aeration. The controlled temperature was reduced from 30℃ gradient to 15℃ (30, 27, 24, 21, 18, 15℃), and then gradually increased to 30℃.Shortcut nitrification sludge was regularly added to SBR² to enhance AOB activity during the cooling and heating process, and SBR1 was used as the control process. The results showed that SBR1 and SBR² started successfully and run stably without shortcut nitrification sludge, and the nitrogen removal efficiency of SBR1 and SBR² was good at 30℃. When the temperature was dropped to 15℃, the concentration of the ammonia nitrogen in effluents of of SBR1 and SBR² were 36.38mg/L and 33.10mg/L, and the total nitrogen removal efficiency was 30.72% and 35.76%, respectively. Both rectors’ efficiency become worse in low temperature settings, SBR² shown a better cold resistance performance. When temperature were increased gradient back to 30℃,the total nitrogen removal rates of SBR1 and SBR² increased back to 52.43% and 63.60% respectively. The activity of bacteria in SBR1 and SBR² decreased with the decrease of temperature, but the AOB activity of SBR² was higher than that of SBR1. During the temperature rising stage, the activity of bacteria in SBR1 and SBR² both increased, and the inhibition of nitrite-oxidizing bacteria (NOB) activity in SBR² was continuously decreasing. The better denitrification performance of SBR² was suspected because when the AOB activity of SBR² was enhanced, more nitrite was produced, and the substrate of Anammox was increased, which resulted in the higher activity abundance of Anammox. Therefore, it was conclude that the AOB activity in SPN/A system can be enhanced by biological addition at low temperature, which can improve the resistance performance of the system to the temperature shocks and facilitate the recovery of denitrification capacity.

Key words： single-stage partial nitrification and anammox municipal wastewater biological addition temperature AOB activity

Received: 28 September 2018

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X703

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	Articles by authors
	WANG Si-meng
	MIAO Yuan-yuan
	PENG Yong-zhen

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WANG Si-meng,MIAO Yuan-yuan,PENG Yong-zhen. Operation characteristics of the SPN/A process for municipal wastewater under low temperature shortcut nitrification sludge[J]. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(4): 1456-1463.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2019/V39/I4/1456

[1]	Sliekers A O, Derwort N, Campos-Gomez J L, et al. Completely autotrophic nitrogen removal over nitrite in one single reactor[J]. Water Research, 2002,36(10):2475-2482.
[2]	Qiao S, Tian T, Duan X M, et al. Novel single-stage autotrophic nitrogen removal via co-immobilizing partial nitrifying and anammox biomass[J]. Chemical Engineering Journal, 2013,230:19-26.
[3]	Joss A, Salzgeber D, Eugster J, et al. Full-scale nitrogen removal from digester liquid with partial nitritation and anammox in one SBR[J]. Environmental Science & Technology, 2009,43(14):5301-5306.
[4]	van der Star W R L, Abma W R, Blommers D, et al. Startup of reactors for anoxic ammonium oxidation:experiences from the first full-scale anammox reactor in Rotterdam[J]. Water Research, 2007,41(18):4149-4163.
[5]	Lv Y T, Chen X, Wang L, et al. Microprofiles of activated sludge aggregates using microelectrodes in completely autotrophic nitrogen removal over nitrite (CANON) reactor[J]. Frontiers of Environmental Science & Engeering, 2016,10(2):390-398.
[6]	Abma W R., Driessen W, Haarhuis R, et al. Upgrading of sewage treatment plant by sustainable and cost-effective separate treatment of industrial wastewater[J]. Water Science and Technology, 2010,61(7):1715-1722.
[7]	Ali M, Okabe S. Anammox-based technologies for nitrogen removal:Advances in process start-up and remaining issues[J]. Chemosphere, 2015,141:144-153.
[8]	Regmi P, Miller M W, Holgate B, et al. Control of aeration, aerobic SRT and COD input for mainstream nitritation/denitritation[J]. Water Research, 2014,57:162-171.
[9]	Ma B, Wang S Y, Cao S B, et al. Biological nitrogen removal from sewage via anammox:Recent advances[J]. Bioresource Technology, 2016,200:981-990.
[10]	Wang T, Zhang H, Gao D, et al. Enrichment of Anammox bacteria in seed sludges from different wastewater treating processes and start-up of Anammox process[J]. Desalination, 2011,271(1-3):193-198.
[11]	Cao Y S, van Loosdrecht M C, Daigger G T, Mainstream partial nitritation-anammox in municipal wastewater treatment:status, bottlenecks, and further studies[J]. Applied Microbiology and Biotechnology, 2017,101(4):1365-1383.
[12]	Ma B, Bao P, Wei Y, et al. Suppressing nitrite-oxidizing bacteria growth to achieve nitrogen removal from domestic wastewater via anammox using intermittent aeration with low dissolved oxygen[J]. Scientific Reports, 2015,5:13048.
[13]	Yang Q, Peng Y Z, Liu X H, et al. Nitrogen removal via nitrite from municipal wastewater at low temperatures using real-time control to optimize nitrifying communities[J]. Environmental Science & Technology, 2007,41(23):8159-8164.
[14]	Xu G J, Zhou Y, Yang Q, et al. The challenges of mainstream deammonification process for municipal used water treatment[J]. Applied Microbiology and Biotechnology, 2015,99(6):2485-2490.
[15]	Miao L, Wang S Y, Li B K, et al. Effect of carbon source type on intracellular stored polymers during endogenous denitritation (ED) treating landfill leachate[J]. Water research, 2016,100:405-412.
[16]	Miao Y Y, Zhang L, Yang Y D, et al. Start-up of single-stage partial nitrification-anammox process treating low-strength swage and its restoration from nitrate[J]. Bioresource Technology, 2016,218:771-779.
[17]	Regmi P, Bunce R, Miller M W, et al. Ammonia-based intermittent aeration control optimized for efficient nitrogen removal[J]. Biotechnology and Bioengineering, 2015,112(10):2060-2067.
[18]	Wett B, Omari A, Podmirseg S M, et al. Going for mainstream deammonification from bench to full scale for maximized resource efficiency[J]. Water Science and Technology, 2013,68(2):283-289.
[19]	Perez J, Lotti T, Kleerebezem R, et al. Outcompeting nitrite-oxidizing bacteria in single-stage nitrogen removal in sewage treatment plants:a model-based study[J]. Water Research, 2014,66:208-218.
[20]	Lotti T, Kleerebezem R, Hu Z, et al. Pilot-scale evaluation of anammox-based mainstream nitrogen removal from municipal wastewater[J]. Environmental Technology, 2015,36(9-12):1167-1177.
[21]	Cema G, Plaza E, Trela J, et al. Dissolved oxygen as a factor influencing nitrogen removal rates in a one-stage system with partial nitritation and Anammox process[J]. Water Science and Technology, 2011,64(5):1009-1015.
[22]	Trojanowicz K, Plaza E, Trela J. Pilot scale studies on nitritationanammox process for mainstream wastewater at low temperature[J]. Water Science and Technology, 2016,73(4):761-768.
[23]	唐晓雪.生活污水厌氧氨氧化组合处理工艺及过程控制[D]. 北京:北京工业大学, 2014. Tang X X, Autotrophic nitrogen removal process treating domestic wastewater based on real-time control[D]. Beijing:Beijing University of Technology, 2014.
[24]	Schmid M C, Maas B, Dapena A, et al. Biomarkers for in situ detection of anaerobic ammonium-oxidizing (anammox) bacteria. Applied and Environmental Microbiology, 2005,71(4):1677-1684.
[25]	Geets J, de Cooman M, Wittebolle L, et al. Real-time PCR assay for the simultaneous quantification of nitrifying and denitrifying bacteria in activated sludge[J]. Applied Microbiology and Biotechnology, 2007,75(1):211-221.
[26]	Degrange V, Bardin R, Detection and counting of nitrobacter populations in soil by PCR[J]. Applied and Environmental Microbiology, 1995,61(6):2093-2098.
[27]	Wang S Y, Wang Y, Feng X J, et al. Quantitative analyses of ammonia-oxidizing Archaea and bacteria in the sediments of four nitrogen-rich wetlands in China[J]. Applied Microbiology and Biotechnology, 2011,90(2):779-787.
[28]	Miao Y Y, Zhang L, Li B K, et al. Enhancing ammonium oxidizing bacteria activity was key to single-stage partial nitrification-anammox system treating low-strength sewage under intermittent aeration condition[J]. Bioresource Technology, 2017,231:36-44.
[29]	王亚娥,冯娟娟,李杰,等.不同Fe(Ⅲ)对活性污泥异化铁还原耦合脱氮的影响及机理初探[J]. 环境科学学报, 2014,34(2):377-384 Wang Y E, Feng J J, Li J, et al. Effect and mechanism of nitrogen removal by dissimilatory reduction of different Fe(Ⅲ) in activated sludge[J]. Acta Scientiae Circumstantiae, 2014,34(2):377-384.
[30]	张宇坤,王淑莹,董怡君,等.NaCl盐度对氨氧化细菌活性的影响及动力学特性[J]. 中国环境科学, 2014,35(2):465-470 Zhang Y K, Wang S Y, Dong Y J, et al. Effect of NaCl salinity on activity of ammonia-oxidizing bacteria and kinetic characterization[J]. China Environmental Science, 2015,35(2):465-470.
[31]	杨庆,杨玉兵,杨忠启,等.溶解氧对短程硝化稳定性及功能菌群的影响[J]. 中国环境科学, 2018,38(9):3328-3334. Yang Q, Yang Y B, Yang Z Q, et al. Effect of dissolved oxygen on the stability and functional microbial communities of the partial nitrification[J]. China Environmental Science, 2018,38(9):3328-3334.
[32]	张功良,李冬,张肖静,等.低温低氨氮SBR短程硝化稳定性试验研究[J]. 中国环境科学, 2014,34(3):610-616. Zhang G L, Li D, Zhang X J, et al. Stability for shortcut nitrification in SBR under low ammonia atlow temperature[J]. China Environmental Science, 2014,34(3):610-616.
[33]	赵昕燕,卞伟,侯爱月,等.季节性温度对短程硝化系统微生物群落的影响[J]. 中国环境科学, 2017,37(4):1366-1374. Zhao X Y, Bian W, Hou A Y, et al. Characteristics of microbial community structure in the stable operation of the partial cut nitrification system with seasonal temperature[J]. China Environmental Science, 2017,37(4):1366-1374.