信号分子在好氧颗粒污泥形成过程中的作用

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Abstract

The correlation analysis based on the physical and chemical properties of sludge, the removal efficiency of pollutants, the variation of extracellular polymeric substances (EPS) and signal molecules during the granulation process were carried out. It was notable that the contaminants removal efficiency was significantly improved. Compared with activated sludge, the removal rates of COD, NH₄⁺-N and PO₄^3--P increased by factors of 20%, 36% and 57% in mature aerobic granular sludge. The extracellular protein (PN) and polysaccharide (PS) content increased by 116 and 31mg/gMLVSS, respectively. On the basis of laser confocal scanning microscopy (CLSM) analysis, it was remarkable that the protein from EPS increased significantly, which indicated that the protein may play an important role in the granulation process. The activity of phosphodiesterase showed an upward trend, and the second messenger cyclic diguanylic (c-di-GMP) content increased during formation and decreased when AGS became mature, which was consistent with the variation of protein and polysaccharide in EPS. Moreover, according to the SPSS analysis, c-di-GMP has a significantly positive correlation with proteins in Tightly Bound-EPS (TB-EPS). It was speculated that c-di-GMP may accelerate the formation of aerobic granular sludge by regulating the biosynthesis of proteins in TB-EPS.

Key words： aerobic granular sludge extracellular polymeric substances (EPS) protein and polysaccharide c-di-GMP phosphodiesterase

Received: 03 September 2018

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X522

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	Articles by authors
	WANG Yu-ying
	ZHI Li-ling
	MA Xin-xin
	WANG Shuo
	LI Ji

Cite this article:

WANG Yu-ying,ZHI Li-ling,MA Xin-xin等. Characteristics of signal molecules in the formation of aerobic granular sludge[J]. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(4): 1516-1524.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2019/V39/I4/1516

[1]	Adav S S, Lee D, Show K, et al. Aerobic granular sludge:Recent advances[J]. Biotechnology Advances, 2008,26(5):411-423.
[2]	王硕,于水利,徐巧,等.好氧颗粒污泥特性、应用及形成机理研究进展[J]. 应用与环境生物学报, 2014,20(4):732-742. Wang S, Yu S l, Xu Q, et al. Characteristics, application and formation mechanisms of aerobic granular sludge:recent advances[J]. Chinese Journal of Applied & Environmental Biology, 2014,20(4):732-742.
[3]	金正宇,郗皓,苑泉,等.进水模式对强化脱氮好氧颗粒污泥培养的影响[J]. 中国环境科学, 2018,38(3):935-942. Jin Z Y, Xi H, Yuan Q, et al. Impacts of feeding mode on stable aerobic granular sludge cultivation for enhanced denitrification[J]. China Environmental Science, 2018,38(3):935-942.
[4]	Ni B, Yu H. Mathematical modeling of aerobic granular sludge:A review[J]. Biotechnology Advances, 2010,28(6):895-909.
[5]	Salehizadeh H, Yan N. Recent advances in extracellular biopolymer flocculants[J]. Biotechnology Advances, 2014,32(8):1506-1522.
[6]	Whiteley C G, Lee D. Bacterial diguanylate cyclases:Structure, function and mechanism in exopolysaccharide biofilm development[J]. Biotechnology Advances, 2015,33(1):124-141.
[7]	Pronk M, de Kreuk M K, de Bruin B, et al. Full scale performance of the aerobic granular sludge process for sewage treatment[J]. Water Reserch. 2015,84:207-217.
[8]	Guo Y, Liu S, Tang X, et al. Role of c-di-GMP in anammox aggregation and systematic analysis of its turnover protein in Candidatus Jettenia caeni[J]. Water Research, 2017,113:181-190.
[9]	李玖龄.基于信号分子AHLs检测的微氧废水处理系统脱氮机制研究[D]. 哈尔滨:哈尔滨工业大学, 2016. Li J L. The mechanism of nitrogen removal in a microaerobic wasterwater treatment system based on the decetion of signal molecule[D]. Harbin:Harbin Institute of Technology, 2016.
[10]	那路新,杨振军.环二鸟苷酸(c-di-GMP)在微生物体内的作用及其类似物的研究[J]. 药学学报, 2012,23(3):307-312. Lu X Z, Liu Z J, Yu W G.Cyclic Diguanylate-New Second Messenger in Bacteria[J]. Acta Pharmaceutica Sinica, 2012,23(3):307-312.
[11]	Chen Z, Schaap P. The prokaryote messenger c-di-GMP triggers stalk cell differentiation in Dictyostelium[J]. Nature, 2012,488(7413):680-683.
[12]	Christen B, Christen M, Paul R, et al. Allosteric control of cyclic di-GMP signaling[J]. Journal of Biological Chemistry, 2006,281(42):32015-32024.
[13]	Karaolis D, Rashid M H, Chythanya R, et al. c-di-GMP (3'-5'-cyclic diguanylic acid) inhibits Staphylococcus aureus cell-cell interactions and biofilm formation[J]. Antimicrobial Agents and Chemotherapy, 2005,49(3):1029-1038.
[14]	Nakhamchik A, Wilde C, Rowe-Magnus D A. Cyclic-di-GMP regulates extracellular polysaccharide production, biofilm formation, and rugose colony development by Vibrio vulnificus[J]. Applied and Environmental Microbiology, 2008,74(13):4199-4209.
[15]	编委会国家环境保护总局水和废水监测分析方法.水和废水监测分析方法(第四版)[M]. 北京:中国环境科学出版社, 2009. Editorial Board of State Environmental Protection Administration & The Analysis Method of water and wastewater. The method of monitoring and analysis about water and wastewater (4th Edition)[M]. Bei Jing:China Environmental Press, 2009.
[16]	Liang Z, Li W, Yang S, et al. Extraction and structural characteristics of extracellular polymeric substances (EPS), pellets in autotrophic nitrifying biofilm and activated sludge[J]. Chemosphere, 2010,81(5):626-632.
[17]	朱芸芸,李敏,曲博,等.湿地植物根际土壤磷酸酶活性变化规律研究[J]. 环境科学与技术, 2016,39(10):106-112. Zhu Y Y, Li M, Qu B, et al Research on the Variations of Phosphatase Activity in Rhizosphere Soil of Wetland Plants[J]. Environmental Science & Technology, 2016,39(10):106-112.
[18]	Simm R, Morr M, Kader A, et al. GGDEF and EAL domains inversely regulate cyclic di-GMP levels and transition from sessility to motility[J]. Molecular Microbiology, 2004,53(4):1123-1134.
[19]	Chen M Y, Lee D J, Tay J H. Distribution of extracellular polymeric substances in aerobic granules[J]. Applied Microbiology and Biotechnology. 2007,73(6):1463-1469.
[20]	廖青,李小明,杨麒,等.好氧颗粒污泥的快速培养以及胞外多聚物对颗粒化的影响研究[J]. 工业用水与废水, 2008,38(4):13-19. Liao Q, Li X M, Yang Q, et al. Rapid cultivation of aerobic granular sludge and influence of extracellular polymeric substances on granulation[J]. Industrial Water & Wastewater, 2008,38(4):13-19.
[21]	Liu Y, Tay J H. The essential role of hydrodynamic shear force in the formation of biofilm and granular sludge[J]. Water Research, 2002, 36(7):1653-1665.
[22]	Badireddy A R, Chellam S, Gassman P L, et al. Role of extracellular polymeric substances in bioflocculation of activated sludge microorganisms under glucose-controlled conditions[J]. Water Research, 2010,44(15):4505-4516.
[23]	Deng S, Wang L, Su H. Role and influence of extracellular polymeric substances on the preparation of aerobic granular sludge[J]. Journal of Environmental Management, 2016,173:49-54.
[24]	王陆玺,周楠,王晨旭,等.流体流速对好氧颗粒污泥快速培养的影响[J]. 中国环境科学, 2018,38(6):2090-2096. Wang L X, Zhou N, Wang C X, et al. Influences of fluid flow rate on the rapid culture of aerobic granular sludge[J]. China Environmental Science. 2018,38(6):2090-2096.
[25]	Xie B, Gu J, Lu J. Surface properties of bacteria from activated sludge in relation to bioflocculation[J]. Journal of Environmental Sciences, 2010,22(12):1840-1845.
[26]	Badireddy A R, Chellam S, Gassman P L, et al. Role of extracellular polymeric substances in bioflocculation of activated sludge microorganisms under glucose-controlled conditions[J]. Water Research, 2010,44(15):4505-4516.
[27]	Manikandan K, Sabareesh V, Singh N, et al. Two-Step Synthesis and Hydrolysis of Cyclic di-AMP in Mycobacterium tuberculosis[J]. Plos One. 2014,9(4):e965904.
[28]	Du B, Ji W, An H, et al. Functional analysis of c-di-AMP phosphodiesterase, GdpP, in Streptococcus suis serotype 2[J]. Microbiological Research. 2014,169(9/10):749-758.
[29]	Wan C, Zhang P, Lee D, et al. Disintegration of aerobic granules:Role of second messenger cyclic di-GMP[J]. Bioresource Technology, 2013,146:330-335.