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| Effect of c-di-GMP in the formation of aerobic granular sludge at low temperature |
| ZHI Li-ling1,2, WANG Yu-ying1,2, MA Xin-xin1,2, YI Xue-song3, WANG Shuo1,2, LI Ji1,2 |
1. School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China;
2. Water Treatment Technology and Technology in Jiangsu Province Materials Collaborative Innovation Center, Jiangnan University, Suzhou 215009, China;
3. College of Environment and Ecology, Hainan University, Haikou 570228, China |
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Abstract Along with the granulation process of floc sludge, signal molecules can lead to the variations of extracellular polymeric substances (EPS). In order to investigate the variations and effects of cyclic diguanylate (c-di-GMP) in the formation of aerobic granular sludge at low temperatures, the correlation analysis was carried out based on EPS, relative hydrophobicity (RH), zeta potential and community succession. The experimental results showed that EPS content increased from 48 to 139mg/g MLVSS during granulation, among which the significant increase was attributed to tightly bound-EPS (TB-EPS). The c-di-GMP content increased from 62 to 600μg/g MLVSS, which resulted in microbial succession and biofilm formation, and further improved the secretion of EPS and the formation of aerobic granular sludge. In addition, notable discrepancies were discovered in the microbial population, and the communities associated with the biosynthesis of c-di-GMP prevailed during the formation of aerobic granular sludge. The community succession was obvious and stable microbial community was eventually formed at low temperature, which was conducive to good performance of nitrogen and phosphorus removal.
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Received: 11 September 2018
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