氮磷形态与浓度对铜绿微囊藻和斜生栅藻生长的影响

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Abstract

The growth of Microcystis aeruginosa and Scenedesmus obliquus was investigated using sodium nitrate, ammonium chloride and urea as nitrogen (N) source; dipotassium hydrogen phosphate, sodium glycerophosphate and adenosine triphosphate as phosphorus (P) source. The N concentrations of culture media were 1.00, 4.00, 8.00mg/L, respectively, and the P concentrations were 0.20, 2.00mg/L, respectively. Bulk culture experiment was conducted to investigate the growth response of Microcystis aeruginosa and Scenedesmus obliquus to different forms and concentrations of N and P under N and P starvation. The results showed that different forms of N and P can stimulate the growth of two species of algae, but there was a bit difference. There was no significant difference in growth rates of M. aeruginosa between different concentrations of sodium nitrate, while the growth rates of S. obliquus reached the highest growth rate when N concentration of sodium nitrate increased to 4.00mg/L. It indicated that 1.00mg/L sodium nitrate has saturated the N requirement of M. aeruginosa, and the nitrogen demand of S. obliquus was higher than that of M. aeruginosa. The growth rates of M. aeruginosa was same when cultured with 1.00 and 4.00mg/L of ammonium chloride and urea, and the growth rates and maximal cell densities were higher than that cultured with same concentration of sodium nitrate. Compares with sodium nitrate, indicating M. aeruginosa prefers to use ammonium chloride and urea. However, the growth rates of M. aeruginosa cultured by 8.00mg/L ammonium chloride was lower than that cultured with same concentrations of urea and sodium nitrate, and also lower than that cultured with low concentration ammonium chloride, indicating high concentration of ammonium chloride was harmful to the growth of M. aeruginosa. There was no significant difference in growth rate and maximal cell densities of S. obliquus between 8.00mg/L of ammonium chloride and 8.00mg/L of urea. And both were higher than that using sodium nitrate to culture. It indicated that the tolerance of S. obliquus to ammonium chloride was higher than that of M. aeruginosa. All three forms of P can be utilized by M. aeruginosa and S. obliquus. However, the maximal cell densities of M. aeruginosa was higher with high concentration of organic phosphorus, and S. obliquus grew better under high concentration of inorganic phosphorus, indicating that availability of different forms of P were different among different algae. The ammonium concentration declined significantly, and inorganic phosphorus concentration was lower than organic phosphorus in recent years in Lake Taihu, which will benefit dominance of cyanobacteria. At present, the concentration of ammonium nitrogen in Taihu Lake is significantly reduced, the proportion of inorganic phosphorus in water is very low, and the concentration of dissolved organic phosphorus is relatively high, which is more favorable to the formation of cyanobacteria.

Key words： nitrogen phosphorus forms Microcystis aeruginosa Scenedesmus obliquus growth rate

Received: 20 November 2018

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X524

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	XU Hai
	CHEN Dan
	CHEN Jie
	ZHU Guang-wei
	QIN Bo-qiang
	ZHU Meng-yuan
	ZHANG Yun-lin

Cite this article:

XU Hai,CHEN Dan,CHEN Jie等. Effects of nitrogen and phosphorus forms and concentrations on the growth of Microcystis aeruginosa and Scenedesmus obliquus[J]. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(6): 2560-2567.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2019/V39/I6/2560

[1]	Paerl H W. Mitigating harmful cyanobacterial blooms in human-and climatically-impacted world[J]. Life, 2014,4(4):988-1012.
[2]	吴庆龙,谢平,杨柳燕,等.湖泊蓝藻水华生态灾害形成机理及防治的基础研究[J]. 地球科学进展, 2008,23(11):1115-1123.Wu Q L, Xie P, Yang L Y, et al. Ecological consequences of cyanobacetrial blooms in lakes and their countermeasures[J]. Advances in Earth Science, 2008,23(11):1115-1123.
[3]	Paerl H W, Hall N S, Calandrino E S. Controlling harmful cyanobacterial blooms in a world experiencing anthropogenic and climatic-induced change[J]. Science of The Total Environment, 2011, 400(10):1739-1745.
[4]	Qin B Q, Xu P Z, Wu Q L, et al. Environmental issues of Lake Taihu[J]. Hydrobiologia, 2007,581(1):3-14.
[5]	Wang P F, Wang Y X, Wang C, et al. Ecological characteristics and environmental factors of phytoplankton during different seasons and in different parts of Taihu Lake[J]. Fundamental and Applied Limnology, 2015,187(1):33-42.
[6]	Li Y P, Tang C Y, Yu Z B, et al. Correlations between algae and water quality:factors driving eutrophication in Lake Taihu, China[J]. International Journal of Environmental Science and Technology, 2014,11(1):169-182.
[7]	O'Neil J M, Davis T W, Burford M A, et al. The rise of harmful cyanobacteria blooms:the potential roles of eutrophication and climate change[J]. Harmful Algae, 2013,14:313-334.
[8]	Conley D J, Paerl H W, Howarth R W, et al. Ecology.Controlling eutrophication:nitrogen and phosphorus[J]. Science, 2009,323(5917):1014-1015.
[9]	曹焕生,孔繁翔,谭啸,等.太湖水华蓝藻底泥中复苏和水柱中生长的比较[J]. 湖泊科学, 2006,18(6):585-589.Cao H S, Kong F X, Tan X, et al. Comparison of recruitment from sediments with pelagic growth of cyanobacteria in Lake Taihu, China[J]. Journal of Lake Sciences, 2006,18(6):585-589.
[10]	朱广伟.太湖富营养化现状及原因分析[J]. 湖泊科学, 2008, 20(1):21-26.Zhu G W. Eutrophic status and causing factors for a large, shallow and subtropical Lake Taihu, China[J]. Journal of Lake Sciences, 2008, 20(1):21-26.
[11]	潘克厚,姜广信.有害藻华(HAB)的发生、生态学影响和对策[J]. 中国海洋大学学报, 2004,34(5):781-786.Pan K H, Jiang G X. The occurrence, ecological effects of HAB and countermeasures against it[J]. Periodical of Ocean University of China, 2004,34(5):781-786.
[12]	Downing J A, Watson S B, McCauley E. Predicting cyanobacteria dominance in lakes[J]. Canadian Journal of Fisheries and Aquatic Sciences, 2001,58:1905-1908.
[13]	吴雅丽,许海,杨桂军,等.太湖春季藻类生长的磷营养盐阈值研究[J]. 中国环境科学, 2013,33(9):1622-1629.Wu Y L, Xu H, Yang G J, et al. Developing the critical phosphorus threshold for spring algal growth in Lake Taihu, China[J]. China Environment Science, 2013,33(9):1622-1629.
[14]	Smith V H. Low nitrogen to phosphorus ratios favor dominance by blue-green algae in lake phytoplankton[J]. Science, 1983,221(4611):669-671.
[15]	Paerl H W, Fulto R S, Moisander P H, et al. Harmful freshwater algal blooms, with an emphasis on cyanobacteria[J]. Scientific World Journal, 2001,1:76-113.
[16]	许海,朱广伟,秦伯强,等.氮磷比对水华蓝藻优势形成的影响[J]. 中国环境科学, 2011,31(10):1676-1683.Xu H, Zhu G W, Qin B Q, et al. Influence of nitrogen-phosphorus ratio on dominance of bloom-forming cyanobacteria (Microcystis aeruginosa)[J]. China Environment Science, 2011,31(10):1676-1683.
[17]	郑晓宇,顾詠洁,金妍,等.不同氮、磷质量浓度下四尾栅藻的生长研究[J]. 生态环境学报, 2010,19(11):2663-2668.Zheng X Y, Gu Y J, Jin Y, et al. Study on the growth of Scenedesmus quadricauda under different nutrients[J]. Ecology and Environment, 2010,19(11):2663-2668.
[18]	许海,吴雅丽,杨桂军,等.铜绿微囊藻、斜生栅藻对氮磷饥饿的耐受能力研究[J]. 生态科学, 2014,33(5):879-884.Xu H, Wu Y L, Yang G J, et al. Tolerance of Microcystis aeruginosa and Scendesmus obliquus to nitrogen and phosphorus deficiency[J]. Ecological Science, 2014,33(5):879-884.
[19]	刘凯峰,肖爱风,刘伟杰,等.氮磷浓度对惠氏栅藻和斜生栅藻生长的影响[J]. 南方水产科学, 2017,13(2):69-76.Liu K F, Xiao A F, Liu W J, et al. Effect of nitrate and phosphate concentration on growth of Microcystis wesenbergii and Scenedesmus obliquus[J]. South China Fisheries Science, 2017,13(2):69-76.
[20]	胡章喜,徐宁,段舜山,等.尿素对中国近海3种典型赤潮藻生长的影响[J]. 环境科学学报, 2010,30(6):1265-1270.Hu Z X, Xu N, Duan S S, et al. Effects of urea on the growth of Phaeocystis globosa, Scrippsiella trochoidea, Skeletonema costatum[J]. Acta Science Circumstantiae, 2010,30(6):1265-1270.
[21]	Bai X L, Ding S M, Fan C X, et al. Organic phosphorus species in surface sediments of a large, shallow, eutrophic lake, Lake Taihu, China[J]. Environmental Pollution, 2009,157(8/9):2507-2513.
[22]	Boyer J N, Dailey S K, Gibson P J, et al. The role of dissolved organic matter bioavailability in promoting phytoplankton blooms in Florida Bay[J]. Hydrobiologia, 2006,569(1):71-85.
[23]	Qian S Q, Kong F X, Shi X L, et al. Interspecific interaction between Microcystis aeruginosa and Chlorella pyrenoidosa in different phosphate media[J]. Journal of Freshwater Ecology, 2008,23(4):635-642.
[24]	Shi X L, Qian S Q, Kong F X, et al. Differences in growth and alkaline phosphatase activity between Microcystis aeruginosa and Chlorella pyrenoidosa in response to media with different organic phosphorus[J]. Journal of Limnology, 2011,70(1):21-25.
[25]	Wang Z H, Liang Y, Kang W. Utilization of dissolved organic phosphorus by different groups of phytoplankton taxa[J]. Harmful Algae, 2011,12:113-118.
[26]	秦伯强,胡维平,陈伟民,等.太湖水环境演化过程与机理[M]. 北京:科学出版社, 2004.Qin B Q, Hu W P, Chen W M, et al. Evolution process and mechanism of lake Taihu environment[M]. Beijing:Science Press, 2004.
[27]	杨柳,章铭,刘正文.太湖春季浮游植物群落对不同形态氮的吸收[J]. 湖泊科学, 2011,23(4):605-611.Yang L, Zhang M, Liu Z W. Uptake of various forms of nitrogen by phytoplankton community in spring in Lake Taihu[J]. Journal of Lake Sciences, 2011,23(4):605-611.
[28]	周涛,李正魁,冯露露.氨氮和硝氮在太湖水华自维持中的不同作用[J]. 中国环境科学, 2013,33(2):305-311.Zhou T, Li Zheng Z K, Feng L L. The different roles of ammonium and nitrate in the bloom self-maintenance of Lake Taihu[J]. China Environmental Science, 2013,33(2):305-311.
[29]	Jacoby J M, Collier D C, Welch E B, et al. Environmental factors associated with a toxic bloom of Microcystis aeruginosa[J]. Canadian Journal of Fisheries and Aquatic Sciences, 2000,57(1):231-240.
[30]	张青田,王新华,林超,等.不同氮源对铜绿微囊藻增殖的影响[J]. 水生态学杂志, 2011,32(4):115-120.Zhang Q T, Wang X H, Lin C, et al. Effects of different nitrogen on proliferation of Microcystis aeruginosa[J]. Journal of Hydroecology, 2011,32(4):115-120.
[31]	唐全民,陈峰,向文洲,等.铵氮对铜绿微囊藻(Microcystis aeroginosa) FACHB905的生长、生化组成和毒素生产的影响[J]. 暨南大学学报(自然科学版), 2008,29(3):290-294.Tang Q M, Chen F, Xiang W Z, et al. Effect of ammonium nitrogen on the growth, biochemical constitutes and toxin production of Microcystis aeroginosa FACHB905[J]. Journal of Jinan University (Naturnal Science), 2008,29(3):290-294.
[32]	Teubner K, Dukulill M T. 14C-photosynthesis of phytoplankton in an oligotrophic Alpine Lake (Traunsee, Austria) and its response to turbidity caused by industrial tailings[J]. Water, Air and Soil Pollution:Focus, 2002,2(4):181-190.
[33]	Spodniewska I. Nitrogen and phosphorus forms in water and the food requirements of algae[J]. Wiadomosci Ekologiczne, 1974,19(3):238-244.
[34]	张民,史小丽,蒋丽娟,等.两种外源性磷及振荡对铜绿微囊藻(Microcystis aeruginosa)生长的影响[J]. 应用与环境生物学报, 2002,8(5):507-510.Zhang M, Shi X L, Jiang L J, et al. Effects of two exogenous phosphous and shake on the growth of Microcystis aeruginosa[J]. 2002,8(5):507-510.
[35]	吕伟伟,姚昕,张保华,等.基于地统计学分析的太湖颗粒态和溶解态氮、磷营养盐时空分布特征及来源分析[J]. 环境科学, 2019, 40(2):590-602.Lü W W, Yao X, Zhang B H, et al. Temporal-spatial distribution of nitrogen and phosphorus nutrients in Lake Taihu based on geostatistical analysis[J]. Environmental Science, 2019,40(2):590-602.
[36]	冯露露,李正魁,周涛.太湖浮游植物和各形态无机氮的时空分布特征[J]. 湖泊科学, 2012,24(5):739-745.Feng L L, Li Z K, Zhou T. Temporal and spatial distributions of phytoplankton and various forms of inorganic nitrogen in Lake Taihu[J]. Journal of Lake Sciences, 2012,24(5):739-745.
[37]	金相灿,屠清瑛.湖泊富营养化调查规范[M]. 北京:中国环境出版社, 1990.Jing X C, Tu Q Y. Specifications for lake eutrophication survey[M]. Beijing:China Environmental Science Press, 1990.
[38]	Muro-Pastor M I, Florencio F J. Regulation of ammonium assimilation in cyanobacteria[J]. Plant Physiology and Biochemistry, 2003,41:595-603.
[39]	孟顺龙,王菁,裘丽萍,等.氮磷质量浓度对普通小球藻和鱼腥藻生长竞争的影响[J]. 生态环境学报, 2015,24(4):658-664.Meng S L, Wang J, Qiu L P, et al. Effect of nitrogen and phosphorus mass concentrations on the growth and competition of Chlorella vulgaris and Anabaenasp. strain PCC[J]. Ecology and Environment Sciences, 2015,24(4):658-664.
[40]	Tilman D, Kiesling R, Sterner R, et al. Green, bluegreen and diatom algae:taxonomic differences in competitive ability for phosphorus, silicon and nitrogen[J]. Archiv für Hydrobiologie, 1986,106(4):473-485.
[41]	Michard M, Aleya L, Verneaux J. Mass occurrence of the cyanobacteria Microcystis aeruginosa in the hypereutrophic Villrest Resevoir (Roanne, France):Usefulness of biyearly examination of N/P (nitrogen phosphorus) and P/C (protein/carbohydrate) couplings[J]. Archiv für Hydrobiologie, 1996,135(3):337-359.
[42]	Giani A, Delgado PCS. Growth dynamics and competitive ability of green (Oocystis lacustris) and a blue-green alga (Synechocystis sp.) under different N:P ratios[J]. Verhein der Internationalen Vereinigung von Limnologie, 1998,26:1693-1697.
[43]	Azov Y, Goldman J C. Free ammonia inhibition of algal photosynthesis in intensive cultures[J]. Applied and Environmental Microbiology, 1982,43(4):735-739.
[44]	Grover J P, Roelke D L, Brooks B W,et al. Ammonium treatments to suppress toxic blooms of Prymnesium parvum in a subtropical lake of semi-arid climate:Results from in situ mesocosm experiments[J]. Water Research, 2013,47:4274-4285.
[45]	Dai G Z, Shang J L, Qiu B S. Ammonia may play an important role in the succession of cyanobacterial blooms and the distribution of common algal species in shallow freshwater lakes[J]. Global Change Biology, 2012,18:1571-1581.
[46]	颜昌宙,曾阿妍,金相灿,等.不同浓度氨氮对轮叶黑藻的生理影响[J]. 生态学报, 2007,27(3):1050-1055.Yan C Y, Zeng A Y, Jin X C, et al. Physiological effects of ammonia-nitrogen concentrations on Hydrilla verticillata[J]. Acta Ecologica Sinica, 2007,27(3):1050-1055.
[47]	Ke Z X, Xie P, Guo L G. Controling factors of spring-summer phytoplankton succession in Lake Taihu(Meiliang Bay, China)[J]. Hydrobioogia, 2008,607:41-49.
[48]	王敏,张建云,陈求稳,等.太湖西北湖区2003~2012年间氮磷浓度及浮游植物主要类群变化趋势分析[J].生态学报, 2019,39(1):164-172.Wang M, Zhang J Y, Chen Q W, et al. Variations in nitrogen and phosphorous concentrations and major phytoplankton species in the northwestern Lake Taihu between 2003~2012[J]. Acta Ecologica Sinica, 2019,39(1):164-172.
[49]	Rückert G V, Giani A. Effect of nitrate and ammonium on the growth and protein concentration of Microcystis viridis Lemmermann (Cyanobacteria)[J]. Revista Brasileira de Botanica, 2004,27(2):325-331.
[50]	许海,杨林章,刘兆普.铜绿微囊藻和斜生栅藻生长的氮营养动力学特征.环境科学研究, 2008,21(1):69-73.Xu H, Yang L Z, Liu Z P. Dynamics study on the effect of nitrogen on the growth of Microcystis aeruginosa and Scendesmus obliquus[J]. Research of Environmental Sciences, 2008,21(1):69-73.
[51]	Anderson S. Applied research/demonstration of hog manure as a fertilizer source in east-central Saskatchewan[J]. 2002.
[52]	Richardson T L, Pinckney J L, Parel H W, et al. Responses of estuarine phytoplankton communities to nitrogen form and mixing using microcosm bioassays[J]. Estuaries and Coasts, 2001,24(6):828-839.
[53]	Håkanson L, Bryhn A C, Hytteborn J K, et al. On the issue of limiting nutrient and predictions of cyanobacteria in aquatic systems[J]. Science of the total environment, 2007,379(1):89-108.
[54]	Schindler D W. Evolution of phosphorus limitation in lakes[J]. Science, 1977,195(4275):260-262.
[55]	Schindler D W. Factors regulating phytoplankton production and Standing crop in the world's freshwaters[J]. Limnology Oceanography, 1978,23(3):478-486.
[56]	高光,秦伯强,朱光伟,等.太湖梅梁湾中碱性磷酸酶的活性及其与藻类生长的关系[J]. 湖泊科学, 2014,16(3):245-251.Gao G, Qin B Q, Zhu G W, et al. Seasonal variation of alkaline phosphatase activity in Meiliang Bay, Lake Taihu[J]. Journal of Lake Sciences, 2014,16(3):245-251.
[57]	黄邦钦,王海黎,洪华生,等.厦门海域浮游植物和细菌对溶解有机磷的利用[J]. 厦门大学学报(自然科学版), 1996,35(4):625-630.Huang B Q, Wang H L, Hong H S, et al. Uptake of dissolved organic phosphorus compounds by phytoplankton and bacterioplankton in Xiamen seawaters[J]. Journal of Xiamen University (Natural Science), 1996,35(4):625-630.
[58]	黄世玉,黄邦钦.不同磷源对藻类生长和生化组成的影响[J]. 台湾海峡, 1997,16(4):458-464.Huang S Y, Huang B Q. Effects of different phosphorus sources on algal growth and biochemical composition[J]. Journal of Oceanography In Taiwan Strait, 1997,16(4):458-464.
[59]	王海黎,洪华生,黄邦钦.海洋环境中溶解有机磷的生物活性初探[J]. 厦门大学学报(自然科学版), 1995,34(3):416-420.Wang H L, Hong H S, Huang B Q. A preliminary study on the bioactivty of dissolved organic phosphorus in marine environment[J]. Journal of Xiamen university (Natural Science), 1995,34(3):416-420.
[60]	许海,杨林章,茅华,等.铜绿微囊藻、斜生栅藻生长的磷营养动力学特征[J]. 生态环境, 2006,15(5):921-924.Xu H, Yang L Z, Mao H, et al. Dynamic studies on the effect of phosphorus on the growth of Microcystis aeruginosa and Scendesmus obliquus[J]. Ecology and Environment, 2006,15(5):921-924.
[61]	金相灿,郑朔方.有机磷和无机磷对铜绿微囊藻生长的影响及动力学分析[J]. 环境科学研究, 2006,19(5):40-44.Jin X C, Zheng S F. The effect of organic and inorganic phosphorous on the growth of Microcystis Aerugiosa and its dynamic analysis[J]. Research of Environmental Sciences, 2006,19(5):40-44.
[62]	沈宏,林俊,郑振华,等.有机磷农药对铜绿微囊藻生长及摄磷效应的动力学研究[J]. 水生生物学报, 2004,28(2):174-179.Shen H, Lin J, Zheng Z H, et al. Kinetic studies on the effects of organo phosphorus pesticides on the growth of Microcystis a. and its uptake to phosphorus[J]. Acta Hydrobiologica Sinica, 2004,28(2):174-179.
[63]	金相灿,叶春,颜昌宙,等.太湖重点污染控制区综合治理方案研究[J]. 环境科学研究, 1999,12(5):1-5.Jin X C, Ye C, Yan C Y, et al. Comprehensive treatment plan for key polluted regions of Lake Taihu[J]. Research of Environmental Sciences, 1999,12(5):1-5.