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| Genetic diversity and phylogenetic analysis of Microcystis in Poyang Lake based on MLST |
| LIU Man-sen1, CAI Fang-fang2,3, WANG Yi-lang2,3, HUO Da2,3, YU Gong-liang2, LI Shou-chun1, LI Ren-hui2 |
1. Colege of Life Science, Jiangxi Normal University, Nanchang 330500, China;
2. Key Laboralory of Algae Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
3. University of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract Using the multilocus sequence typing (MLST) based on seven housekeeping loci (ftsZ, glnA, gltX, gyrB, pgi, recA and tpi), twenty Microcystis strain isolated from Lake Poyang, the largest freshwater Lake in China, were examined to study their genetic diversity and phylogenetic relationship. The MLST revealed the 20 unique sequence types (STs) for these 20 Microcystis strains and their higher genetic diversity with H value as 0.986. The phylogenetic analysis based on the seven gene sequences showed that Microcystis strains were divided into five different groups, Phylogenetic tree based on the STs from our research and Japanese lakes revealed that our STs formed the independent branch, indicating that microcysts from different areas is relatively stable. So it can be clearly characterized by MLST.
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Received: 17 December 2018
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