绿脓菌素促进铜绿假单胞菌NY3降解烃的作用机制

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Abstract

The secretion of pyocyanin (Pyo) is one of the most significant characteristics of Pseudomonas aeruginosa NY3. Previous experiments have observed that Pyo secretion is often positively correlated with hydrocarbon degradation efficiency, but its role in pollutant degradation has not been concerned, and the mechanism is unclear. Based on the coexistence of glutaric acid as a high-secreting Pyo system and reagent-grade Pyo standard, the effects of Pyo on intracellular redox enzyme activity, specific enzyme activity and extracellular electron transfer rate during hydrocarbon degradation by NY3 bacteria were studied. Glutaric acid could significantly increase the secretion of Pyo, which was 86.6% higher than that of non-glutaric acid system, and with the increase of Pyo secretion, the removal rate of hexadecane by NY3 bacteria increased by 16.29%. Pyo secretion by NY3 bacteria was beneficial to increase the activity of intracellular alkane oxidase. When the bacteria grew to 72h and the dosage of Pyo was 200L and 300L, respectively, compared with that without Pyo system, alkane was removed by NY3 bacteria. The specific activity of hydrocarbon oxidase increased by 121.8% and 346.5% respectively. At the same time, in the presence of Pyo, the extracellular electron transfer rate was increased by nearly 7 times, which increased the degradation rate of hexadecane. Based on this, we propose that Pyo secretion by NY3 bacteria can promote the degradation of hexadecane by increasing the activity of intracellular enzymes and the rate of extracellular electron transfer.

Key words： Pseudomonas aeruginosa NY3 pyocyanine pyo hydrocarbons alkane oxidase glutaric acid

Received: 05 December 2018

PACS:

X172

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	ZHANG Bo
	NIE Mai-qian
	NIE Hong-yun
	TIAN Xiao-ting
	QIAO Qi

Cite this article:

ZHANG Bo,NIE Mai-qian,NIE Hong-yun等. The effects of pyocyanin on alkanes degradation by Pseudomonas aeruginosa NY3[J]. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(7): 3088-3093.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2019/V39/I7/3088

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