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Isolation and denitrification characteristics of heterotrophic nitrification-aerobic denitrification bacterium HY3-2 |
YANG Jing-dan1, ZHU Ming-han1, LIU Lin1, Lü Min2, LI Si-qi1, WANG Xiao-hui1 |
1. College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China;
2. Poten Environmental Group Co., Ltd, Beijing 100082, China |
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Abstract A heterotrophic nitrification-aerobic denitrification bacterium named HY3-2 was isolated from the high-salt aerobic granular sludge system. The morphological observation and 16S rDNA sequence analysis showed that HY3-2 was Klebsiella quasipneumoniae subsp.quasipneumoniae. The removal characteristics of ammonia nitrogen, nitrate and nitrite by HY3-2 were studied. The results showed that the strain had good heterotrophic nitrification and aerobic denitrification performance, and the removal rates of ammonia nitrogen, nitrate and nitrite were 63.57%, 88.11% and 98.38% respectively. The study on the nitrogen removal performance of the strain showed that:HY3-2 used glycerol as carbon source. Under the condition (C/N 25, temperature 20℃ or 30℃, rotation speed 150r/min, initial pH 7~10, salinity less than 50g/L), the effect on NH4+-N removal with the concentration of 100mg/L was good, and the removal rate was 90.7%. When the sodium citrate was used as carbon source (C/N 25, temperature 30℃, rotation speed 150r/min, initial pH 5~9), the aerobic denitrification can be performed when the salinity was less than 15g/L, the NO3--N removal rate was over 99%.
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Received: 06 June 2019
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[1] |
Robertson L A, Kuenen J G, Kleijntjens R. Aerobic denitrification and heterotrophic nitrification by Thiosphaera pantotropha[J]. Antonie Van Leeuwenhoek, 1985,51(4):445-445.
|
[2] |
Richardson D J, Wehrfritz J M, Keech A, et al. The diversity of redox proteins involved in bacterial heterotrophic nitrification and aerobic denitrification[J]. Biochemical Society Transactions, 1998,26(3):401-408.
|
[3] |
Ji B, Yang K, Zhu L, et al. Aerobic denitrification:A review of important advances of the last 30years[J]. Biotechnology and Bioprocess Engineering, 2015,20(4):643-651.
|
[4] |
曾薇,张丽敏,王安其,等.污水处理系统中硝化菌的菌群结构和动态变化[J]. 中国环境科学, 2015,35(11):3257-3265. Zeng W, Zhang L M, Wang Q A, et al. Community structures and population dynamics of nitrifying bacteria in activated sludges of wastewater treatment plants[J]. China Environmental Science, 2015,35(11):3257-3265.
|
[5] |
Fujiwara T, Fukumori Y. Cytochrome cb-type nitric oxide reductase with cytochrome c oxidase activity from Paracoccus denitrificans ATCC 35512[J]. Journal of Bacteriology, 1996,178(7):1866-1871.
|
[6] |
Zhang Q L, Liu Y, Ai G M, et al. The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium, Bacillus methylotrophicus strain L7[J]. Bioresource Technology, 2012, 108(3):35-44.
|
[7] |
王国英,崔杰,岳秀萍,等.异养硝化-好氧反硝化菌脱氮同时降解苯酚特性[J]. 中国环境科学, 2015,35(9):2644-2649. Wang G Y, Cui J, Yue X P, et al. Simultaneous removal of phenol and ammonium by a heterotrophic nitrifiying-aerobic denitrifying bacterium[J]. China Environmental Science, 2015,35(9):2644-2649.
|
[8] |
Yang J X, Gao S S, Ma F, et al. Identification and phylogenetic analysis of an isolated aerobic denitrifier[J]. Acta Scientiae Circumstantiae, 2008,28(7):1302-1307.
|
[9] |
Hu Z, Lee J W, Chandran K, et al. Nitrous oxide (N2O) emission from aquaculture:A review[J]. Environmental Science & Technology, 2012,46(12):6470-6480.
|
[10] |
Jin R, Liu T, Liu G, et al. Simultaneous heterotrophic nitrification and aerobic denitrification by the marine origin bacterium Pseudomonas sp. ADN-42[J]. Applied Biochemistry and Biotechnology, 2015,175(4):2000-2011.
|
[11] |
肖继波,江惠霞,褚淑祎.不同氮源下好氧反硝化菌Defluvibacter lusatiensis str. DN7的脱氮特性[J]. 生态学报, 2012,32(20):6463-6470. Xiao J B, Jiang H X, Chu S Y. Denitrification characteristics of an aerobic denitrifying bacterium Defluvibacter lusatiensis str.DN7using different sources of nitrogen. Acta Ecologica Sinica, 2012,32(20):6463-6470.
|
[12] |
刘天琪,金若菲,周集体,等.异养硝化-好氧反硝化菌ADN-42的脱氮特性[J]. 环境工程学报, 2015,9(2):989-996. Liu T Q, Jin R F, Zhou J T, et al. Nitrogen-removal characteristic of heterotrophic nitrification and aerobic denitrification bacterium ADN-42[J]. Chinese Journal of Environmental Engineering, 2015, 9(2):989-996.
|
[13] |
应启锋.直链烷基苯磺酸盐(LAS)的生物降解性[J]. 微生物学通报, 2002,29(5):85-95. Ying Q F. The biodegradation of linear alkylbenzenesulphonate (LAS)[J]. Microbiology China, 2002,29(5):85-95.
|
[14] |
Firth J R, Edwards C. Analysis of denitrification by Pseudomonas stutzeri under nutrient-limited conditions using membrane inlet mass spectrometry[J]. Journal of Applied Microbiology, 2010,88(5):853-859.
|
[15] |
Joo H S, Hirai M, Shoda M. Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis No. 4[J]. Journal of Bioscience and Bioengineering, 2005, 100(2):184-191.
|
[16] |
Joo H S, Hirai M, Shoda M. Piggery wastewater treatment using Alcaligenes faecalis strain No. 4with heterotrophic nitrification and aerobic denitrification[J]. Water Research, 2006,40(16):3029-3036.
|
[17] |
Zhang J, Wu P, Hao B, et al. Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001[J]. Bioresource Technology, 2011,102(21):9866-9869.
|
[18] |
Pedersen H, Dunkin K A, Firestone M K. The relative importance of autotrophic and heterotrophic nitrification in a conifer forest soil as measured by 15N tracer and pool dilution techniques[J]. Biogeochemistry, 1999,44(2):135-150.
|
[19] |
Patureau D, Bernet N J P, Delgenès, et al. Effect of dissolved oxygen and carbon-nitrogen loads on denitrification by an aerobic consortium[J]. Applied Microbiology & Biotechnology, 2000,54(4):535-542.
|
[20] |
赵惊鸿.高温好氧反硝化菌的筛选及其脱氮性能研究[D]. 广州:华南理工大学, 2014. Zhao J H. Isolation and characteristics of aerobic denitrifying bacterium in thermophilic conditions[D]. Guangzhou:South China University of Technology, 2014.
|
[21] |
Dincer A R, Kargi F. Salt inhibition of nitrification and denitrification in saline wastewater[J]. Environmental Technology, 1999,20(11):1147-1153.
|
[22] |
唐婧,肖亚男,屈姗姗,等.一株耐盐好氧反硝化细菌的分离鉴定及其脱氮特性[J]. 环境工程学报, 2014,8(12):5499-5506. Tang J, Xiao Y N, Qu S S, et al. Identification and denitrification characteristics of a strain of halophilic denitrifying bacteria[J]. Chinese Journal of Environmental Engineering, 2014,8(12):5499-5506.
|
[23] |
Van Niel E W J. Heterotrophic nitrification and aerobic denitrification in Alcaligenes faecalis strain TUD[J]. Antonie Van Leeuwenhoek, 1992,62(3):231-237.
|
[24] |
He T, Li Z, Sun Q, et al. Heterotrophic nitrification and aerobic denitrification by Pseudomonas tolaasii Y-11 without nitrite accumulation during nitrogen conversion[J]. Bioresource Technology, 2016,200:493-499.
|
|
|
|