重金属离子对肺表面活性物质单层膜的影响

Abstract
Figure/Table
References (36)
Related Citation (15)

Download: PDF (1108 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract

In order to investigate the effect of heavy metals on pulmonary surfactant (PS) membranes, the interaction between different types and concentrations of heavy metal ions and PS monolayer membranes were studied using Langmuir-Wilhelmy membrane balances. The six heavy metal ions of Cr³⁺, Cu²⁺, Hg²⁺, Pb²⁺, Cd²⁺ and Ni²⁺ all expanded the π-A isotherm G-LE phase transition phase of PS monolayer, while Fe³⁺ induced monolayer condensation. Subsequently, Cd²⁺, Cu²⁺, Hg²⁺and Fe³⁺ were selected to further investigate the effect of heavy metal concentration on the surface activity of PS monolayer films,the compressible modulus analysis was carried out, and the mechanism of action was explored by combining infrared spectroscopy (FTIR) and atomic force microscope (AFM). Cu²⁺, Fe³⁺ and Hg²⁺ all cooperated with the polar head of PS monolayer in a concentration-dependent manner to form a complex and change the stability of the membrane, while the effect of Cd²⁺ was obviously weaker than the other three metal ions, but it could also enhance the PS monolayer order.

Key words： pulmonarysurfactant heavy metal ion π-A isotherm compressible modulus infrared spectrum atomic force microscope

Received: 18 July 2019

PACS:

X503.1

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	XU Lin-zhen
	ZHAO Qun
	TIAN Sen-lin
	LI Ying-jie
	ZHANG Lin-feng
	CHAI Xiao-long
	GENG Ying-xue
	CAO Yan

Cite this article:

XU Lin-zhen,ZHAO Qun,TIAN Sen-lin等. Effect of heavy metal ions on the surface activity of pulmonary surfactant monolayer membrane[J]. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(2): 857-864.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2020/V40/I2/857

[1]	Kastury F, Smith E, Juhasz A L. A critical review of approaches and limitations of inhalation bioavailability and bioaccessibility of metal (loid) s from ambient particulate matter or dust[J]. Science of the Total Environment, 2017,574:1054-1074.
[2]	Hu G, Jiao B, Shi X, et al. Physicochemical properties of nanoparticles regulate translocation across pulmonary surfactant monolayer and formation of lipoprotein corona[J]. ACS Nano, 2013,7(12):10525-10533.
[3]	Gonzalez D H, Cala C K, Peng Q, et al. HULIS enhancement of hydroxyl radical formation from Fe(II):, kinetics of fulvic acid-Fe(II) complexes in the presence of lung anti-oxidants.[J]. Environmental Science & Technology, 2017,51(13):7676-7685.
[4]	周林,邵龙义,刘君霞,等.宣威肺癌高发区室内PM10对肺泡上皮细胞凋亡的影响[J]. 中国环境科学, 2010,30(7):1004-1008. Zhou L, Shao L Y, Liu J X, et al. Effects of indoor PM10 on alveolar epithelial cell apoptosis in Xuanwei lung cancer-prone areas[J]. China Environmental Science, 2010,30(7):1004-1008.
[5]	Baoukina S, Tieleman D P. Computer simulations of lung surfactant[J]. Biochimicaet BiophysicaActa (BBA)-Biomembranes, 2016, 1858(10):2431-2440.
[6]	Wüstneck R, Perez-Gil J, Wüstneck N, et al. Interfacial properties of pulmonary surfactant layers[J]. Advances in Colloid & Interface Science, 2005,117(1-3):33-58.
[7]	Palleschi S, Rossi B, Armiento G, et al. Toxicity of the readily leachable fraction of urban PM2.5, to human lung epithelial cells:Role of soluble metals[J]. Chemosphere, 2018,196:35-44.
[8]	Kramer BW. The respiratory distress syndrome (RDS) in preterm infants[J]. Intensivmedizin und Notfallmedizin, 2007,44(7):403-408.
[9]	Eduardo G, Liggieri L, Santini E, et al. DPPC-DOPC Langmuir monolayers modified by hydrophilic silica nanoparticles:Phase behaviour, structure and rheology[J]. Colloids and Surfaces A:Physicochemical and Engineering Aspects, 2012,413:174-183.
[10]	Tang C Y, Huang Z, Allen H C. Binding of Mg2+ and Ca2+ to palmitic acid and deprotonation of the COOH headgroup studied by vibrational sum frequency generation spectroscopy.[J]. Journal of Physical Chemistry B, 2010,114(51):17068-76.
[11]	Shannon L S, Brown N J, Dohm M T, et al. Lipid composition greatly affects the in vitro surface activity of lung surfactant protein mimics[J]. Colloids and Surfaces B:Biointerfaces, 2007,57(1):37-55.
[12]	董声焕.肺表面活性物质基础与临床[M]. 北京:人民军医出版社, 2012:32-46. Dong S H. Basics and clinical characteristics of pulmonary surfactant[M]. Beijing:People's Military Medical Publishing House, 2012:32-46.
[13]	Bruni R, Waring T A J. Surfactant protein B:Lipid interactions of synthetic peptides representing the amino-terminal amphipathic domain[J]. Proceedings of the National Academy of Sciences of the United States of America, 1991,88(16):7451-7455.
[14]	Casillas-Ituarte N N, Chen X, Castada H, et al. Na+and Ca2+ effect on the hydration and orientation of the phosphate group of DPPC at air-water and air-hydrated silica interfaces[J]. Journal of Physical Chemistry B, 2010,114(29):9485-95.
[15]	Adams E M, Casper C B, Allen H C. Effect of cation enrichment on dipalmitoylphosphatidylcholine (DPPC) monolayers at the air-water interface[J]. Journal of Colloid and Interface Science, 2016,478:353-364.
[16]	Zhao Q, Li Y J, Chai X L, et al. Interaction of inhalable volatile organic compounds and pulmonary surfactant:Potential hazards of VOCs exposure to lung[J]. Journal of hazardous materials, 2019,369:512-520.
[17]	Zhao Q, Wang Q, Li Y J, et al. Influence of volatile organic compounds (VOCs) on pulmonary surfactant monolayers at air-water interface:Implication for the pulmonary health[J]. Colloids and Surfaces A:Physicochemical and engineering Aspects, 2019,562:402-408.
[18]	Zhao Q, Li Y J, Chai X L, et al. Interaction of pulmonary surfactant with silica and polycyclic aromatic hydrocarbons:Implications for respiratory health[J]. Chemosphere, 2019,222:603-610.
[19]	Zhao Q, Li Y J, Chai X L, et al. Interaction of nano carbon particles and anthracene with pulmonary surfactant:The potential hazards of inhaled nanoparticles[J]. Chemosphere, 2019,215:746-752.
[20]	武荣.前体脂质体型猪肺表面活性物质制备和研究[D]. 广州:南方医科大学, 2006. Wu R. Preparation and research of proliposomal porcine lung surfactant[D]. Guangzhou:Southern Medical University, 2006.
[21]	李俊花,孙润广,郝长春,等.细胞色素C/心磷脂相互作用的Langmuir-Blodgett膜界面特性研究[J]. 中国科学:化学, 2015, 45(10):1065-1074. Li J H, Sun R G, Hao C C, et al. Study on Langmuir-Blodgett Membrane Interface Characteristics of Cytochrome C/Cardiolipin Interactions[J]. Chinese Science:Chemistry, 2015,45(10):1065-1074.
[22]	杨小乐. LB膜与AFM技术研究磷脂酰乙醇胺单分子膜结构[J]. 液晶与显示, 2006,21(4):348-356. Yang X L. Study on the structure of phosphatidylethanolamine monolayers by LB film and AFM technology[J]. Liquid Crystals and Display, 2006,21(4):348-356.
[23]	Zeng Z, Li D, Xue W, et al. Structural models and surface equation of state for pulmonary surfactant monolayers[J]. Biophysical Chemistry, 2007,131(1-3):88-95.
[24]	Baumler S M, Blanchard G J. The influence of metal ions on the dynamics of supported phospholipid langmuir films[J]. Langmuir, 2017,33(12):2986-2992.
[25]	Minami H, Inoue T. Aggregation of dipalmitoylphosphatidylcholine vesicles induced by some metal ions with high activity for hydrolysis[J]. Langmuir, 1999,15(20):6643-6651.
[26]	Bergamino M, Relini A, Rispoli P, et al. An EXAFS study of the binding of Cd and Pb ions to lipid films[J]. The European Physical Journal E, 2013,36(9):102-109.
[27]	刘明耀,张亚霏.肺表面活性物质理化特性的检测指标和临床应用[J]. 国外医学,呼吸系统分册, 1986,(1):1-4. Liu M Y, Zhang Y K. Detection indexes and clinical application of physicochemical properties of pulmonary surfactants[J]. Foreign Medical Sciences, Respiratory System Volume, 1986,(1):1-4.
[28]	Adams E M, Verreault D, Jayarathne T, et al. Surface organization of a DPPC monolayer on concentrated SrCl2and ZnCl2solutions[J]. Physical Chemistry Chemical Physics, 2016,16:32-41.
[29]	Le M T, Gailer J, Prenner E J. Hg2+ and Cd2+interact differently with biomimetic erythrocyte membranes[J]. Biometals, 2009,22(2):261-274.
[30]	Suwalsky M, Ungerer B, Villena F, et al. HgCl2 disrupts the structure of the human erythrocyte membrane and model phospholipid bilayers[J]. Journal of Inorganic Biochemistry, 2000,81(4):267-273.
[31]	Duncan S L, Larson R G. Comparing experimental and simulated pressure-area isotherms for DPPC[J]. Biophysical Journal, 2008, 94(8):2965-2986.
[32]	Aoki P H B, Morato L F C, Pavinatto F J, et al. Molecular-level modifications induced by photo-oxidationof lipid monolayers interacting with erythrosin[J]. Langmuir, 2016,32(15):3766-3773.
[33]	Li S Y, Du L, Tsona N T, et al. The interaction of trace heavy metal with lipid monolayer in the sea surface microlayer[J]. Chemosphere, 2018,196:323-330.
[34]	Wang W, Zhang H, Feng S, et al. Iron ion and iron hydroxide adsorption to charge-neutral phosphatidylcholine templates[J]. Langmuir, 2016,32(30):7664-7670.
[35]	Maltseva E, Shapovalov V L, Möhwald H, et al. Ionization state and structure of L-1,2-dipalmitoylphosphatidylglycerol monolayers at the liquid/air interface[J]. The Journal of Physical Chemistry B, 2006,110(2):919-926.
[36]	Yang X M, Xiao D, Xiao S J, et al. Domain structures of phospholipid monolayer Langmuir-Blodgett films determined by atomic force microscopy[J]. Applied Physics A (Solids and Surfaces), 1994,59(2):139-143.