高原高寒污水处理系统的微生物群落特征

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Abstract

To investigate the microbial structure and diversity of the wastewater treatment systems in plateau and cold regions, three plateau wastewater treatment plants (WWTPs) in Lhasa, Yunnan and Sichuan were chosen as experimental group, and two non-plateau WWTPs in Chongqing were used for comparison. Polymerase chain reaction-Denaturing gradient gel electrophoresis (PCR-DGGE) was adapted to reveal the microbial characteristics of plateau and non-plateau WWTPs. The cluster results showed that plateau samples exhibited far distances from non-plateau samples, and the microbial community displayed an apparent difference. The average diversity of plateau WWTPs was lower than that of non-plateau WWTPs, which might be due to the significant inhibitory effect of ultraviolet (UV) radiation in plateau regions. As a result, the pollutant removal efficiency in plateau WWTPs was not satisfied as expected. For community composition, a total of 16genera were identified, which belong to the phyla of Proteobacteria, Bacteroidetes, Firmicutes and Verrucomicrobia. The difference analysis between groups showed that there was only one genus, Prosthecobacter, significantly enriched in plateau group, which is widely distributed in WWTPs and can adapt to the low temperature in the plateau. But for most activated sludge bacteria, high altitude and UV irradiation is an unfavorable living condition. Therefore, shading the UV radiation for the open WWTPs is a potential way to improve the wastewater treatment performance in plateau and cold regions.

Key words： high altitude and cold wastewater treatment microbial diversity community structure ultraviolet

Received: 14 August 2019

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X703

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	FANG De-xin
	JI Fang-ying
	XU Xiao-yi
	XIONG Jing-zhong

Cite this article:

FANG De-xin,JI Fang-ying,XU Xiao-yi等. Microbial community characteristics of wastewater treatment systems in high-altitude and cold regions[J]. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(3): 1081-1088.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2020/V40/I3/1081

[1]	李德生,程国栋.青藏高原地区低温生活污水的深度处理[J]. 化工学报, 2008,59(4):1001-1007. Li D S, Cheng G D. Advanced treatment of cold domestic sewage in Qinghai-Tibet Plateau[J]. CIESC Journal, 2008,59(4):1001-1007.
[2]	田华,王三反,洪雷,等.青藏高原生活污水处理及回用的工艺研究[J]. 环境科学与技术, 2007,30(6):64-65. Tian H, Wang S F, Hong L. Experiment of domestic wastewater treatment and reuse in Qinghai-Tibet plateau area[J]. Environmental Science & Technology, 2007,30(6):64-65.
[3]	郭劲松,潘颖雅,王春燕,等.高原地区CASS工艺处理城镇污水的生产性调试[J]. 土木建筑与环境工程, 2009,31(4):112-116+122. Guo J S, Pan Y Y, Wang C Y, et al. Productive commissioning of CASS process treating sewage in plateau region[J]. Journal of Civil and Environmental Engineering, 2009,31(4):112-116+122.
[4]	马宗凯,李晓燕,任正元.A/A/O工艺在高原地区污水处理工程中的应用[J]. 水处理技术, 2012,38(5):128-131+134. Ma Z K, Li X Y, Ren Z Y. A/A/O process application of wastewater treatment engineering in plateau section[J]. Technology of Water Treatment, 2012,38(5):128-131+134.
[5]	Seib M D, Berg K J, Zitomer H. Influent wastewater microbiota and temperature influence anaerobic membrane bioreactor microbial community[J]. Bioresource Technology, 2016,216:446-452.
[6]	Niu L H, Li Y, Wang P F, et al. Altitude-scale variation in nitrogenremoval bacterial communities from municipal wastewater treatment plants distributed along a 3600m altitudinal gradient in China[J]. Science of the Total Environment, 2016,559:38-44.
[7]	Wu L, Ning D, Zhang B, et al. Global diversity and biogeography of bacterial communities in wastewater treatment plants[J]. Nature Microbiology, 2019,4(7):1183-1195.
[8]	Hu M, Wang X H, Wen X H, et al. Microbial community structures in different wastewater treatment plants as revealed by 454-pyrosequencing analysis[J]. Bioresource Technology, 2012,117:72-79.
[9]	Wang X H, Hu M, Xia Y, et al. Pyrosequencing analysis of bacterial diversity in 14 wastewater treatment systems in China[J]. Applied and Environmental Microbiology, 2012,78(19):7042-7047.
[10]	Ju F, Xia Y, Guo F, et al. Taxonomic relatedness shapes bacterial assembly in activated sludge of globally distributed wastewater treatment plants[J]. Environmental Microbiology, 2014,16(8):2421-2432.
[11]	叶姜瑜,罗固源.微生物可培养性低的生态学释因与对策[J]. 微生物学报, 2005,45(3):478-482. Ye J Y, Luo G Y. Ecological interpretation and related strategies for low culturability of microorganisms[J]. Acta Microbiologica Sinica, 2005,45(3):478-482.
[12]	韩睿,陈来生,李莉,等.PCR-DGGE研究青海农村户用沼气池微生物群落结构[J]. 中国环境科学, 2015,35(6):1794-1804. Han R, Chen L S, Li L, et al. Microbial community of rural household biogas digesters in Qinghai by PCR-DGGE[J]. China Environmental Science, 2015,35(6):1794-1804.
[13]	Wang L, Liu J L, Zhao Q Y, et al. Comparative study of wastewater treatment and nutrient recycle via activated sludge, microalgae and combination systems[J]. Bioresource Technology, 2016,211:1-5.
[14]	Hesham A, Qi R, Yang M. Comparison of bacterial community structures in two systems of a sewage treatment plant using PCRDGGE analysis[J]. Journal of Environmental Sciences, 2011,23(12):2049-2054.
[15]	李国强,薛林贵,莫天录,等.湖泊沉积物微生物多样性研究方法的新进展[J]. 兰州交通大学学报, 2015,34(6):12-16. Li G Q, Xue L G, Mo T L, et al. New progress of research methods for microbial diversity in lake sediment[J]. Journal of Lanzhou Jiaotong University, 2015,34(6):12-16.
[16]	牛凤霞,吉芳英,赵艮,等.龙景湖沉积物的细菌群落垂向分布特征[J]. 中国环境科学, 2017,37(6):2322-2331. Niu F X, Ji F Y, Zhao G, et al. Vertical distribution of bacterial communities in sediments of Longjing Lake[J]. China Environmental Science, 2017,37(6):2322-2331.
[17]	李新,焦燕,代钢,等.内蒙古河套灌区不同盐碱程度的土壤细菌群落多样性[J]. 中国环境科学, 2016,36(1):249-260. Li X, Jiao Y, Dai G, et al. Soil bacterial community diversity under different degrees of saline-alkaline in the Hetao Area of inner Mongolia[J]. China Environmental Science, 2016,36(1):249-260.
[18]	郑涵,田昕竹,王学东,等.锌胁迫对土壤中微生物群落变化的影响[J]. 中国环境科学, 2017,37(4):1458-1465. Zheng H, Tian X Z, Wang X D, et al. Effects of Zn pollution on soil microbial community in field soils and its main influence factors[J]. China Environmental Science, 2017,37(4):1458-1465.
[19]	孙寓姣,陈程,丁爱中,等.官厅水库水质特征及水体微生物多样性的响应[J]. 中国环境科学, 2015,35(5):1547-1553. Sun Y J, Chen C, Ding A Z, et al. The corresponding of microbial diversity on water quality and environmental variables of Guanting Reservoir[J]. China Environmental Science, 2015,35(5):1547-1553.
[20]	LaPara T M, Nakatsu C H, Pantea L M, et al. Stability of the bacterial communities supported by a seven-stage biological process treating pharmaceutical wastewater as revealed by PCR-DGGE[J]. Water Research, 2002,36(3):638-646.
[21]	吉芳英,杨琴,罗固源.实验室自配HACH-COD替代试剂研究[J]. 给水排水, 2003,29(1):17-20. Ji F Y, Yang Q, Luo G Y. Laboratory prepared substitutes for HACHCOD meter[J]. Water & Wastewater Engineering, 2003,29(1):17-20.
[22]	黎睿,王圣瑞,肖尚斌,等.长江中下游与云南高原湖泊沉积物磷形态及内源磷负荷[J]. 中国环境科学, 2015,35(6):1831-1839. Li R, Wang S R, Xiao S B, et al. Sediments phosphorus forms and loading in the lakes of the mid-lower reaches of the Yangtze River and Yunnan Plateau, China[J]. China Environmental Science, 2015,35(6):1831-1839.
[23]	吉芳英,颜海波,何强,等.龙景湖龙景沟汇水区沉积物-水界面氮形态空间分布特征[J]. 中国环境科学, 2015,35(10):3101-3107. Ji F Y, Yan H B, He Q, et al. Distribution of nitrogen speciation at the sediment-water interface in Longjinggou Catchment Area of Longjinghu Lake[J]. China Environmental Science, 2015,35(10):3101-3107.
[24]	Parks D H, Tyson G W, Hugenholtz P, et al. STAMP:statistical analysis of taxonomic and functional profiles[J]. Bioinformatics, 2014,30(21):3123-3124.
[25]	Zhang Y, Carvalho P N, Lv T, et al. Microbial density and diversity in constructed wetland systems and the relation to pollutant removal efficiency[J]. Water Science and Technology, 2016,73(3):679-686.
[26]	崔迪,李昂,王继华,等.非培养技术解析生化系统微生物群落结构[J]. 哈尔滨工业大学学报, 2011,43(10):45-49. Cui D, Li A, Wang J H, et al. Analysis of microbial community structure of biochemical system using uncultured technology[J]. Journal of Harbin Institute of Technology, 2011,43(10):45-49.
[27]	Wu B, Tian J Q, Bai C M, et al. The biogeography of fungal communities in wetland sediments along the Changjiang River and other sites in China[J]. Isme Journal, 2013,7(7):1299-1309.
[28]	Zhou Z, Qiao W M, Xing C, et al. Microbial community structure of anoxic-oxic-settling-anaerobic sludge reduction process revealed by 454-pyrosequencing[J]. Chemical Engineering Journal, 2015,266:249-257.
[29]	Zhang T, Shao M F, Ye L. 454 Pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants[J]. Isme Journal, 2012,6(6):1137-1147.
[30]	Fang D X, Zhao G, Xu X Y, et al. Microbial community structures and functions of wastewater treatment systems in plateau and cold regions[J]. Bioresource Technology, 2018,249:684-693.
[31]	Ansola G, Arroyo P, de Miera L E S. Characterisation of the soil bacterial community structure and composition of natural and constructed wetlands[J]. Science of the Total Environment, 2014,473:63-71.
[32]	Wei W, Isobe K, Nishizawa T, et al. Higher diversity and abundance of denitrifying microorganisms in environments than considered previously[J]. Isme Journal, 2015,9(9):1954-1965.
[33]	Shi X Q, Ng K K, Li X R, et al. Investigation of Intertidal Wetland Sediment as a Novel Inoculation Source for Anaerobic Saline Wastewater Treatment[J]. Environmental Science & Technology, 2015, 49(10):6231-6239.
[34]	Macedo W V, Santos C E D, Guerrero R D S, et al. Establishing simultaneous nitrification and denitrification under continuous aeration for the treatment of multi-electrolytes saline wastewater[J]. Bioresource Technology, 2019,288:121529.
[35]	Spring S, Bunk B, Sproer C, et al. Characterization of the first cultured representative of Verrucomicrobia subdivision 5indicates the proposal of a novel phylum[J]. Isme Journal, 2016,10(12):2801-2816.
[36]	Al-Halbouni D, Traber J, Lyko S, et al. Correlation of EPS content in activated sludge at different sludge retention times with membrane fouling phenomena[J]. Water Research, 2008,42(6/7):1475-1488.
[37]	Zielinska M, Rusanowska P, Jarzabek J, et al. Community dynamics of denitrifying bacteria in full-scale wastewater treatment plants[J]. Environmental Technology, 2016,37(18):2358-2367.
[38]	Gonzalez-Martinez A, Rodriguez-Sanchez A, Garcia-Ruiz M J, et al. Performance and bacterial community dynamics of a CANON bioreactor acclimated from high to low operational temperatures[J]. Chemical Engineering Journal, 2016,287:557-567.
[39]	Xia Y, Kong Y H, Thomsen T R, et al. Identification and ecophysiological characterization of epiphytic protein-hydrolyzing Saprospiraceae ("Candidatus epiflobacter" spp.) in activated sludge[J]. Applied and Environmental Microbiology, 2008,74(7):2229-2238.
[40]	Guo J, Wang J, Qiu Y, et al. Realizing a high-rate sulfidogenic reactor driven by sulfur-reducing bacteria with organic substrate dosage minimization and cost-effectiveness maximization[J]. Chemosphere, 2019,236:124381.
[41]	Li A, Qu Y Y, Zhou J T, et al. Characterization of a newly isolated biphenyl-degrading bacterium, Dyella ginsengisoli LA-4[J]. Applied Biochemistry and Biotechnology, 2009,159(3):687-695.
[42]	Inaba T, Hori T, Navarro R R, et al. Revealing sludge and biofilm microbiomes in membrane bioreactor treating piggery wastewater by non-destructive microscopy and 16S rRNA gene sequencing[J]. Chemical Engineering Journal, 2018,331:75-83.