寒旱区湖泊沉积物中固氮微生物群落特征——以包头南海湖为例

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Abstract

In order to explore the regional differences of the nitrogen-fixing bacteria in lakes of cold-arid areas, the nitrogen-fixing bacteria in the sediments of Nanhai Lake and the interaction relationship between the bacteria and the environmental factors were analyzed using the nitrogen-fixing bacteria specific functional gene nif H. The results demonstated that there existed differences in the composition of the dominant nitrogen-fixing bacterial community among various parts of the Lake, with the dominant bacterial community being mainly composed of 3 phyla (Cyanobacteria, Firmicutes and Proteobacteria) and 10 genera (Cyanobacteria, Chromatium, Clostridium, Anabaena, Rhodospirillum, Anaeromyxobacter, Thiocapsa, Cylindrospermopsis, Rhizobium and Pseudomonas). And Cyanobacteria turned out to be the leading bacteria at the phyla level due to the specific environmental conditions of the cold-arid areas like, the long freezing period and strong ultraviolet radiation. Based on the redundancy analysis, a high level of C/N and TP was shown to be the necessary nutrients for the survival of the nitrogen-fixing bacteria by speeding up the nitrogen-fixing reactions therein, while high levels of TN and NH₃-N, and low pH values could inhibit the growth of nitrogen-fixing bacteria in the surface sediments. To be precise, a high pH value would inhibit the growth of Anabaena, Thiocapsa, Cyanobacteria and Rhodospirillum. In this essay, the potential function of the nitrogen fixation was studied by exploring the distributions of nitrogen-fixing microorganisms in the sediments of cold-arid areas, which could better explain the important role that the nitrogen-fixing microorganisms play in the nitrogen cycle.

Key words： cold-arid Area lake wetland high-throughput sequencing function gene nitrogen-fixing bacteria

Received: 20 November 2019

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X172

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	Articles by authors
	YANG Wen-huan
	ZHANG Yuan
	WANG Zhi-chao
	SHI Da-jun
	LI Wei-ping

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YANG Wen-huan,ZHANG Yuan,WANG Zhi-chao等. Community characteristics of nitrogen-fixing microorganisms in lake sediment—taking Nanhaihu lake as example[J]. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(6): 2674-2682.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2020/V40/I6/2674

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