基于碳源优化的反硝化除磷及微生物特性

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Abstract The effect of sodium acetate/sodium propionate ratios (1:0, 2:1, 1:1, 1:2 and 0:1) on denitrifying phosphorus removal was investigated using anaerobic/anoxic/oxic-sequencing batch reactor (AAO-SBR) by seeding the activated sludge from anaerobic/anoxic/oxic-biological contact oxidation (AAO-BCO) system. The evolution of microbial community structure was also compared by high-throughput sequencing under different ratios. The results showed that the AAO-SBR system revealed high organic matter and denitrifying phosphorus removal abilities under five operating conditions. When the ratio of sodium acetate/sodium propionate was 1:0, COD utilization efficiency was 87.63% while poly β-hydroxyalkanoate (PHAs) of 174mgCOD/gMLSS was synthesized simultaneously in the anaerobic stage, and phosphorus release was up to 31.22mg/L. In the anoxic stage, PO₄^3--P (74%) was removed along with NO₃^--N denitrification (90%), and the utilization rate of PHAs was 72.4%, achieving efficient removals of nitrogen and phosphorus. High-throughput sequencing results showed that the abundance and diversity of microbial community were influenced by different carbon source ratios, among which Proteobacteria (31%~76%), Chloroflexi (1%~26%) and Bacteroidetes (2%~31%) occupied a large proportion. But the microbial diversity was higher when sodium acetate and sodium propionate were co-existed. Phosphate accumulating organisms (PAOs, 21.364%) were dominant with the competition of glycogen accumulating organisms (GAOs, 2.317%) using sodium acetate as the single carbon source.

Key words： AAO-SBR reactor sodium acetate/sodium propionate ratios denitrifying phosphorus removal internal carbon source high-throughput sequencing

Received: 14 November 2019

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X703

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	Articles by authors
	PAN Ting
	ZHANG Miao
	FAN Ya-jun
	LIU Yi-zhong
	PANG Jing-jin
	WANG Yi-xin
	YU Meng

Cite this article:

PAN Ting,ZHANG Miao,FAN Ya-jun等. Denitrifying phosphorus removal and microbial characteristics based on the optimization of carbon sources[J]. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(7): 2901-2908.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2020/V40/I7/2901

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