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| The role of Fas/FasL pathway in apoptosis of PC12 cells induced by fluoride exposure |
| ZHANG Jia-yong1, TANG Le1, YU Qiu-li1, RUAN Qin2, ZHANG Zi-gui2 |
1. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;
2. College of Xing Zhi, Zhejiang Normal University, Jinhua 321004, China |
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Abstract The present study investigated the effects of exposure to fluoride on apoptosis of PC12 cells and aimed to clarify the role of Fas/FasL pathway involved in this effect. The results showed that treatment with NaF at 20, 40, 80, 160mg/L for 12, 24, 36, and 48hours enhanced the activity of PC12 cells, respectively. Treatment with all dosages of NaF for 24hours significantly increased the levels of reactive oxygen species (ROS) and apoptosis rate and dose-dependently up-regulated the gene/protein expression levels of Fas, FasL, Caspase8, FADD, and Caspase3, but down-regulated the gene/protein expression levels of Bid (P < 0.05). These results suggest that Fas/FasL apoptotic pathway plays an important role in the apoptosis of PC12 cells induced by fluoride exposure, in which FADD may be an important target molecule of fluoride exposure-induced apoptosis.
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Received: 18 November 2019
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