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| Study on the convenient and efficient enrichment effect of new modified magnetic beads on low level microorganisms in the environment |
| ZHANG Qi1, CHEN Xue2, CAO Jia-qi2, LI Li-li2, ZHANG Li-ping2, LI Fan-shuang2, MA Xue-zheng2, ZHAO Xin2, LIU Li-juan2, FANG Zhi-qiang2, HU Kong-xin2 |
1. College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China;
2. Institute of Health and Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China |
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Abstract In order to study the monitoring method of low-concentration microorganisms in clean environment, three kinds of low concentrated Gram-positive and negative bacteria, were prepared to evaluate the enriched and concentrated efficiency of microorganisms. The experiment showed the bacteria concentrated by the modified magnetic beads had a higher enrichment and concentration ratio compared with E. coli and S. aureus. After non-specific enrichment of microorganisms in tap water, drinking water and bottled water, there were significant difference among these three types of water for the 16S rDNA gene copy numbers (P<0.01), and the significant difference was also observed in the total number of colonies between the supernatant and the enriched microbial complex (P<0.001). Significant difference between filtering method and magnetic-bead enrichment (P=0.002) for tap water, but no significant differences for drinking and bottled water (P=0.362). The colony counting and 16S rDNA gene copy number of bacteria enriched by magnetic beads were analyzed. The absolute gene copy numbers and the total number of colonies in the magnetic-bead microbial complex were significantly higher than those in the supernatant after magnetic separation (P=0.0001). This method has some high efficiency enrichment effect on microorganisms.
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Received: 02 March 2020
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