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| Impacts of different pollution sources on the microbial community in groundwater at municipal solid waste landfill sites |
| LI Na-ying1,2,3, HAN Zhi-yong1,2,3, WANG Shuang-chao3, LI Long-bo4, HUANG Jin3, ZHAO Juan5 |
1. State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, Chengdu University of Technology, Chengdu 610059, China;
2. State Environmental Protection Key Laboratory of Synergetic Control and Joint Remediation for Soil & Water Pollution, Chengdu University of Technology, Chengdu 610059, China;
3. College of Environment and Ecology, Chengdu University of Technology, Chengdu 610059, China;
4. Chengdu Drainage CO. LTD, Chengdu 610000, China;
5. College of Environment and Civil Engineering, Chengdu University of Technology, Chengdu 610059, China |
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Abstract To better understand impacts of different pollution sources including agricultural, domestic, and landfill sources on the microbial community in the groundwater at municipal solid waste landfill sites in red beds area of Sichuan province, water quality and 16S rRNA sequencing analyses of 11groundwater samples were conducted. Groundwater quality analysis result showed that comparing to the background monitoring well (RDBJ), groundwater quality of the investigated area did not exceed the limitation (GB/T 14848-2017), however, they were affected by pollution sources in the vicinity. The major pollutants in the groundwater of landfill diffusion area (RDPD), agricultural area (RDAS), agricultural-domestic area (RDHP) were NO3-(Pbi=7.320) and Cl-(Pbi=7.136), NO3-(Pbi=15.185), NO3-(Pbi=25.040)and SO42-(Pbi=8.259), respectively. Microbial community diversity analysis results showed that Proteobacteria (43.6%~84.1%) was the dominant phylum, whereas Bradyrhizobium and an unclassified genus of Comanonadaceae were the dominant genera in RDAS groundwater. In RDHP groundwater, an unclassified genus of Methyococcaceae, an unclassified genus of Comamonadaceae and Sulfuritalea were the dominant genera. There was no dominant genus in RDPD groundwater, but the microbial abundancy was higher compared to the ones of the other 3groundwater types. There was no significant difference in the microbial community structures of RDPD, RDHP and RDAS. Moreover, the microbial community structures of RDPD and RDHP were similar. The impacts of environmental factors on groundwater microbial community were SO42- > Cl- > NH4+-N > NO3--N > ORP > pH. Our results indicate that microbial community was mainly affected by the domestic and landfill pollution sources, followed by the agricultural pollution resource, and the natural factors seem to be minor factors. The results will provide a guidance for the environmental assessment and the natural attenuation remediation of groundwater contamination near the landfill sites.
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Received: 19 March 2020
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