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| Comparative study of extracellular electron transfer mechanism mediated by electron transfer mediators in two strains of electricigenic bacteria |
| NIU Yu-wei, YUAN Lin-jiang, LIU Bing-yin, WANG Ru |
| Key Laboratory of Northwest Water Resources, Environment and Ecology, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China |
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Abstract Microbial fuel cells (MFCs) inoculated pure electricigenic bacteria strain Klebsiella oxytoca d7and Shewanella sp. F1 were constructed respectively, and the mechanisms of electron transfer by the electron transfer mediators (ETMs) during their electricity generation were studied. K. oxytoca d7 could only secrete ETMs during its electricigenic respiration, while the Shewanella sp. F1 could secrete ETMs during either its aerobiotic respiration, anaerobic respiration or electricigenic respiration, which indicated that the generation of ETMs was related to the species of electricigens. The redox potentials of the two species of bacteria were equivalent (-250~210mV), which were between that of the NADH and coenzyme Q in respiratory chain. The electrons intercepted by the ETMs were both denoted from the NADH on the intracellular respiratory chain, indicating that the "escape" site of the electron only depended on the ETMs itself. Under the conditions of sufficient or insufficient carbon sources, in terms of the K. oxytoca d7, the amount of electricity produced by the ETMs accounted for 60% and 41% of the total electricity generated, respectively, while that of the Shewanella sp. F1 was 57% and 50%, respectively. Electricigenic respiration of two electricigens was mainly based on the ETMs mechanism. The conversion of anode substrate and the power generation performance of the MFCs was directly affected by the process of electron transfer mediated by the ETMs.
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Received: 26 March 2020
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