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| Effect of modified coal gangue on Phosphorus in overlying water and key genes of phosphorus metabolism in sediment |
| ZHOU Jian-min1, FU Yong-sheng1, ZHANG Meng-yao1, DING Shu-hang1, PAN Sheng-wang2, LIU Yi-qing1, WANG Hong-bin2 |
1. School of Geoscience and Environmental Engineering, Southwest Jiaotong University, Chengdu 611756, China;
2. School of Architecture and Environmental Engineering, Chengdu University, Chengdu 610100, China |
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Abstract Thermal activated coal gangue and lanthanum modified coal gangue were used in the experiments of phosphorus removal and fixation in closed water. 16SrRNA high-throughput sequencing technology was used to analyze the changes of microbial community structure, phosphorus accumulating microorganisms and phosphorus metabolism function genes in sediment. Lanthanum modified coal gangue had the best removal ability of total phosphorus (TP) from the overlying water. The concentration of TP in the overlying water was 0.023~0.028 mg/L in the stable period, which was 83.5% lower than that in the control group. The removal ability of TP from the overlying water by thermal activated coal gangue was poor and its concentration in the overlying water in the stable period was about 0.15mg/L, which was slightly lower than that in the control group. Thermal activated coal gangue and lanthanum modified coal gangue both improved the microbial diversity in the sediment.Proteobacteria and Chloroflexa were the dominant bacteria in the sediment. The phosphorus accumulating organisms in the sediment in different treatment groups were Tetraspeaera and Candidatus_Accumulibacter, and their relative abundance was significantly reduced by lanthanum modified caol gangue.these two modified coal gangue both had little effect on polyphosphate kinase (PPK), but an obvious inhibition on polyphosphate exonuclease (PPX) especially for thermal activated coal gangue.
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Received: 04 April 2020
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