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| Factors affecting bio-electrokinetic remediation efficiency of pyrene contaminated saline-alkali soil |
| FAN Rui-juan1,2, MA Yan1, ZHANG Xiu1,2, LIU Ya-qin1,2 |
1. College of Biological Science & Engineering, North Minzu University, Yinchuan 750021, China;
2. Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, Yinchuan 750021, China |
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Abstract Using pyrene-contaminated saline-alkali soil as the test soil, the microbial community structure in the process of bio-electrokinetic remediation (BIO-EK) of pyrene was monitored by high-throughput sequencing technology, combined with the variation characteristics of pyrene concentration and microenvironment, the correlation among microbial community structure, pollutant concentration and soil microenvironment during BIO-EK remediation was analyzed. The results showed that the pyrene concentration in BIO-EK decreased from 288.03 mg/kg to 73.40 mg/kg after 91 days, while the bioremediation (BIO) and electrokinetics (EK) reduced the concentration of pyrene to 114.23 and 150.27 mg/kg, respectively. The degradation rate of pyrene was higher in the early stage, but gradually decreased with the extension of the treatment time. Meanwhile, the application of electric field caused obvious changes in some soil environmental factors (temperature, moisture content and pH) and microbial community structure, and the change of microbial community structure was the main reason that pyrene could not be degraded continuously and efficiently. Therefore, BIO-EK remediation process could be controlled by regulating soil microenvironment and constructing high-efficiency degrading bacteria, which is expected to achieve the aim of continuous and efficient degradation of pollutants.
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Received: 10 July 2020
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