PPARγ/UCP2在甲醛致学习记忆障碍中的作用

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Abstract To investigate the possible role of peroxisome proliferators-activated receptor γ/uncoupling protein 2(PPARγ/UCP2) in learning and memory impairment induced by formaldehyde(FA), C57BL/6 mice were randomly divided into different groups including control group, T0070907group(inhibitor group), 3mg/m³ FA group, 3mg/m³ FA+T0070907 group. After 21 days of continuous exposure to FA or other treatments in the experiment, mouse brain tissue was taken to determine brain index and before being homogenized for the detection of a series of physiological indices such as reactive oxygen species(ROS), glutathione(GSH), malondialdehyde(MDA), nuclear factor κB(NF-κB), interleukin-6(IL-6), PPARγ, UCP-2, as well as the determination of pathological changes by observing the Nissl stained brain tissue. The results showed that compared with those of mice in the control group, the cerebral cortex neurons of the mice were damaged in the T0070907group and the 3mg/m³ FA group, the content of GSH decreased, and the levels of ROS, MDA, NF-κB, and IL-6 increased, with even more severe results were found in mice from the 3mg/m³ FA+T0070907 group. In addition, compared with the control group, the contents of PPARγ and UCP2 decreased in the brain tissue of mice in the T0070907 group, while increased in those from the 3mg/m³ FA group; compared with those of mice from the 3mg/m³ FA group, the contents of PPARγ and UCP2 decreased in the brain tissue of mice from the 3mg/m³ FA+T0070907 group after the inhibitor was administered. The content of PPARγ/UCP2 decreased after addition of T0070907, the inhibitor of PPARγ, which aggravated the learning and memory impairment in mice induced by FA exposure, therefore PPARγ/UCP2 may be protective to mice from the learning and memory impairment induced by FA.

Key words： formaldehyde learning and memory oxidative damage inflammation

Received: 19 November 2020

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	ZHENG Li-fang
	XIAO Jia-li
	YUAN Pei-xin
	CHEN Bo-qi
	MEI Qiong
	CHI Yu-yao
	LV Ke
	LI Rui

Cite this article:

ZHENG Li-fang,XIAO Jia-li,YUAN Pei-xin等. Role of the PPARγ/UCP2 in learning and memory impairment induced by formaldehyde[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(6): 2923-2931.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I6/2923

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