[C<sub>8</sub>mim]Cl对HepG2细胞凋亡和内质网应激通路的影响

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Abstract In order to explore whether ionic liquid 1-octyl-3-methylimidazolium chloride ([C₈mim]Cl) can induce apoptosis through endoplasmic reticulum stress(ERS) pathway, HepG2 cells were treated with 0,50,100,200μmol/L [C₈mim]Cl for 24h based on the MTT cell viability assay. The apoptosis was detected by flow cytometry and western blot was used to assay the expressions of ERS related proteins. The results showed that [C₈mim]Cl increased the apoptosis in HepG2 cells with a concentration-dependent manner. [C₈mim]Cl significantly increased the expressions of ERS related proteins, including glucose regulated protein 78 (GRP78), phosphorylated protein kinase RNA-like endoplasmic reticulum kinase (p-PERK), phosphorylated eukaryotic initiation factor 2α (p-eIF2α), phosphorylated inositol-requiring enzyme 1 (p-IRE1), activating transcription factor 4 (ATF4) and ATF6. [C₈mim]Cl also significantly induced the expressions of C/EBP homologous protein (CHOP) and caspase 4 protein, lead to the increase of caspase 9 and caspase 3 activities. Therefore, [C₈mim]Cl could induce apoptosis in HepG2 cells through ERS pathway.

Key words： [C₈mim]Cl apoptosis ERS CHOP caspase 4

Received: 20 November 2020

PACS:

X171.5

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	Articles by authors
	ZHANG Bang-jun
	TU Zhen-peng
	FENG Yi-yi
	LIU Yang
	LIXiao-yu

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ZHANG Bang-jun,TU Zhen-peng,FENG Yi-yi等. Effects of [C₈mim]Cl on apoptosis and endoplasmic reticulum stress pathway in HepG2 cells[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(6): 2932-2938.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I6/2932

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