菌株WYT降解还原蓝4的关键基因

Abstract
Figure/Table
References
Related Citation (5)

Download: PDF (896 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract A dye degrading bacteria named WYT was isolated from printing and dyeing activated sludge, and the key genes involved in the degradation and decolorization of VB4dye were investigated. The whole genome sequencing and homologous recombination were used to identify the possible key genes of dye metabolism. The gene was knocked out, meanwhile, the vector was constructed for gene complement, and a phenotypic verification experiment was designed to verify the decolorization effect of the gene. The results showed that the possible key gene, named DyP, belonged to type B of DyP eroxidase gene.The DyP knockout strain had no decolorization effect on VB4. The decolorization rate of VB4 was 96.04% in the complementary strain and 96.95% in the wild type, indicating the decolorization rate of VB4 in the complementary strain was restored, DyP gene was the key gene of VB4 degradation and decolorization.

Key words： pseudomonas aeruginosa vat blue 4 degradation decoloring key genes

Received: 09 December 2020

PACS:

X703

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	MA Qian
	LI Hai-hong
	WANG Yang-tao

Cite this article:

MA Qian,LI Hai-hong,WANG Yang-tao. The key genes involved in degradtion of vat blue 4 in bacterial strain WYT[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(7): 3283-3290.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I7/3283

[1]	Benkhaya B, El Harfi S, El Harfi A. Classifications properties and applications of textile dyes:a review[J]. Applied Journal of Environmental Engineering Science, 2017,3(3):311-320.
[2]	Šekuljica N Ž, Prlainović N Ž, Stefanović A B, et al. Decolorization of anthraquinonic dyes from textile effluent using horseradish peroxidase:optimization and kinetic study[J]. The Scientific World Journal, 2015. 2015:371625-371625.
[3]	Li H H, Wang Y T, Wang Y, et al. Bacterial degradation of anthraquinone dyes[J]. Journal of Zhejiang University-SCIENCE B, 2019,20(6):528-540.
[4]	范迪.印染废水处理机理与技术研究[D]. 青岛:中国海洋大学, 2008. Fan D. The study of treatment mechenism and thecnology for the dying and printing wastewater[D]. Qingdao:Ocean University of China, 2008.
[5]	Xu H, Yang B, Liu Y B, et al. Recent advances in anaerobic biological processes for textile printing and dyeing wastewater treatment:a mini-review[J]. World Journal of Microbiology and Biotechnology, 2018,34(11):1-9.
[6]	Hussan M, Iqbal N, Adeel S, et al. Microwave-assisted enhancement of milkweed (calotropis procera L.)leaves as an eco-friendly source of natural colorants for textile[J]. Environmental Science and Pollution Research, 2017,24(5):5089-94.
[7]	Zhang T, X Fei, Wang S, et al. Pigmentation of vat blue RS by ball milling in solvents[J]. Dyes and Pigments, 2017,45(1):15-21.
[8]	Ali H. Biodegradation of synthetic dyes-a review[J]. Water Air and Soil Pollution, 213(1-4):251-273.
[9]	Pathak V V, Kothari R, Chopra A K, et al. Experimental and kinetic studies for phycoremediation and dye removal by Chlorella pyrenoidosa from textile wastewater[J]. Journal of Environmental Management, 2015,163:270-277.
[10]	Rybczynska-Tkaczyk K, Kornillowicz-Kowalska T. Biosorption optimization and equilibrium isotherm of industrial dye compounds in novel strains of microscopic fungi[J]. International Journal of Environmental Science and Technology, 2016,13(12):2837-2846.
[11]	池振明,白玉谦.偶氮染料及其还原产物的微生物降解[J]. 山东大学学报(自然科学版), 1988,(1):94-102. Chi Z M, Bai Y Q. Microbial degradation of azo dyes and their reduction products[J]. Journal of Shandong University (Natural Science), 1988,(1):94-102.
[12]	Velayutham K, Madhava A K, Pushparaj M, et al. Biodegradation of remazol brilliant blue R using isolated bacterial culture (staphylococcus sp. K2204)[J]. Environmental Technology, 2018,39(22):2900-2907.
[13]	杨芳,谢学辉,刘娜等.混合菌群FF降解染料效果及生物毒性变化[J]. 安全与环境学报, 2017,17(2):654-659. Yang F, Xie X H, Liu L, et al. On the effects and biotoxicity variations as a result of dye biodegradation by bacterial consortium FF[J]. Journal of Safety and Environment, 2017,17(2):654-659.
[14]	杨帅,陈立伟,顾海莎,蔡天明.DMF降解菌MBYD-1的分离、鉴定及降解特性[J]. 中国环境科学, 2011,31(3):437-442. Yang S, Chen L W, Gu H S, et al. Isolation, identification and degradation characteristics of a DMF-degrading bacterial MBYD-1[J]. China Environmental Science, 2011,31(3):437-442.
[15]	连榕.庆大霉素生物合成特色基因克隆与表达的研究[D]. 福州:福州大学, 2018. Lian R. The research of cloning and expression of genes involved in gentamicin biosynthesis[D]. Fuzhou:Fuzhou University, 2018.
[16]	Sangha V, Angela B L, Ian O. Identification and cloning of the kinin receptor in the chagas disease vector, rhodnius prolixus[J]. General and Comparative Endocrinology, 2019,289:113380-113380.
[17]	何毅.红色红曲菌M7高效基因敲除体系的构建及桔霉素生物合成途径的解析[D]. 武汉:华中农业大学, 2015. He Y. Construction of highly efficient gene knockout system for monascus ruber M7 and analysis of citrinin biosynthetic pathway[D]. Wuhan:Huazhong Agricultural University, 2015.
[18]	Kang X Y, Li C Q, Luo Y. Cloning of pAhX22, a small cryptic plasmid from aeromonas hydrophila, and construction of a pAhX22-derived shuttle vector[J]. Plasmid, 2020,108:102490-202494.
[19]	Li D Y, Zheng C, Zhou J, et al. Pgp-b2e, a recombinant compatible TA/TB-ligation vector for rapid and inexpensive gene cloning[J]. Molecular Biotechnology, 2020,62(1):56-66
[20]	Tian J J, Zhang Y, Zhu L J, et al. DsDNA/ssDNA-switchable isothermal colorimetric biosensor based on a universal primer and λ exonuclease[J]. Sensors and Actuators:B. Chemical, 2020,323:128674-128699.
[21]	Zuo L H, Zhang S, Zhang J, et al. Primer development and functional classification of EST-SSR markers in ulmus species[J]. Tree Genetics & Genomes, 2020,16(5):71-83.
[22]	Wang R, Chen R, Qian C, et al. Ultrafast visual nucleic acid detection with crispr/cas12a and rapid pcr in single capillary[J]. Sensors and Actuators:B. Chemical, 2021,326:128618-128648.
[23]	王敏敏,赵婕,常亚磊,等.双酶切和无缝克隆方法构建HCV CORE表达载体的比较[J]. 山西医药杂志, 2017,46(3):267-270. Wang M M, Zhao J, Chang Y L, et al. Comparison of the construction of a core-green vector using conventional double enzyme restrictionand and recombination[J]. Shanxi Medical Journal, 2017, 46(3):267-270.
[24]	Liu Y F, Su A Q, Tian R Z, et al. Developing rapid growing bacillus subtilis for improved biochemical and recombinant protein production[J]. Metabolic Engineering Communications, 2020,11:141-141.
[25]	Hend A, Yuka O, Tomoya K, et al. A new escherichia coli entry vector series (pIIS18) for seamless gene cloning using type IIs restriction enzymes.[J]. Microbiology resource announcements, 2019,8(41):319-323.
[26]	顾洪雁,袁晓伟.一种热激PCR法高效鉴定重组克隆[J]. 生物学杂志, 2010,27(2):36-39. Gu H Y, Yuan X W. A highly effective heat shock pcr screening method for recombination clone[J]. Journal of Biology, 2010,27(2):36-39.
[27]	隋欣,任发政,鲁言文,等.热激法修饰瑞士乳杆菌的参数优化与特性研究[J]. 农业机械学报, 2009,40(8):111-114,79. Sui X, Ren F Z, Lu Y W, et al. Parameters optimization and properties for lactobacillus helveticus by heat-shock treatment[J]. Transactions of the Chinese Society for Agricultural Machinery, 2009,40(8):111-114,79.
[28]	Song L, Shao Y F, Ning S X, et al. Performance of a newly isolated salt-tolerant yeast strain pichia occidentalis g1for degrading and detoxifying azo dyes[J]. Bioresource Technology, 2017,233:21-29.
[29]	任随周,郭俊,王亚丽,等.细菌脱色酶TpmD对三苯基甲烷类染料脱色的酶学特性研究[J]. 微生物学报, 2006,(3):385-389. Ren S Z, Guo J, Wang Y L, et al. Properties of a triphenylmethane dyes decolorization enzyme TpmD from aeromonas hydrophila strain dn322[J]. Acta Microbiologica Sinica, 2006,(3):385-389.
[30]	Brown Margaret E, Barros Tiago, Chang Michelle C Y. Identification and characterization of a multifunctional dye peroxidase from a lignin-reactive bacterium[J]. ACS chemical biology, 2012,7(12):2074-2081.
[31]	Smulevich G, Howes B D, Droghetti E. Structural and functional properties of heme-containing peroxidases:a resonance raman perspective for the superfamily of plant, fungal and bacterial peroxidases[J]. Heme Peroxidases, 2016,4:61-98.
[32]	Kim S J, Ishikawa K, Hirai M, et al. Characteristics of a newly isolated fungus, geotrichum candidum dec 1, which decolorizes various dyes[J]. Journal of Fermentation & Bioengineering, 1995,79(6):601-607.
[33]	孙玉雯,崔承彬.抗生素抗性筛选在微生物菌株选育中的作用[J]. 国际药学研究杂志, 2008,(3):213-217. Sun Y W, Cui C B. Antibiotic-resistance mutation technique in microorganism breeding[J]. Journal of International Pharmaceutical Research, 2008,(3):213-217.
[34]	王雪,黄建忠,李力.基因敲除技术在微生物中的应用[J]. 微生物学杂志, 2019,39(4):100-106. Wang X, Huang J Z, Li L. Advances in gene knockout techniques in microbiology[J]. Journal of Microbiology, 2019,39(4):100-106.
[35]	林丹枫,李力,柯崇榕,等,辅酶Q10的生物合成及其高表达研究进展[J]. 生物技术通讯, 2012,1(3):465-469. Lin D F, Li L, Ke C R, et al. Advance of coenzyme Q10 biosynthesis and high expression[J]. Letters in Biotechnology, 2012,1(3):465-469.
[36]	韩武洋,刘金雷,杜红燕,等.谷氨酸棒状杆菌葡萄糖代谢阻断工程菌的构建[J]. 食品科学, 2017,38(2):65-67. Han W Y, Liu J L, Du H Y, et al. Construction of engineered strain blocking glucose metabolism in corynebacterium glutamicum[J]. Food Science, 2017,38(2):65-67.
[37]	Walker G M, Weatherley L R. Biodegradation and biosorption of acid anthraquinone dye[J]. Environmental Pollution, 2000,108(2):219-223.