高原城市室内灰尘对人角膜上皮细胞损伤研究

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Abstract The indoor dust samples were collected from houses, office, and dormitory in a typical high-altitude city Kunming. Each sample was separated into 4size fractions (F1~F4: 100~2000, 75~100, 63~75, 45~63μm), and the concentrations of nine heavy metals (Pb, Cu, Cd, Zn, Cr, Ni, Co, As, and Mn) in indoor dust were detected using ICP-OES. In addition, the solubility rates (bioaccessibility) of the target heavy metals in artificial tears extraction and their cytotoxicity on human corneal epithelial cells (HCECs) were determined. The results showed that Ni, Mn, and Zn in office dust were significantly higher than those in residence and dormitory dust (P<0.01), and the contents of Cd, Ni, and Zn were 39.3, 9.5 and 12.3times higher than the background values of Kunming soils. The solubility rates of Co in residence dust, As in office dust and Mn in dormitory dust were the highest at 10.6%, 16.1%, and 36%, respectively. Heavy metals were highly susceptible to accumulate in office and dormitory dust with particle size ranging from 63 to 100μm. Moreover, the typical polygonal and cobblestone appearance of HCECs became spindle shape after artificial tear extracts of office dust exposure. Cell proliferation was enhanced at 20μg/mL whereas inhibited at higher concentration (P<0.05). The elevated mRNA expression of pro-inflammatory mediators (IL-1β and TNF-α) suggested that indoor dust from Kunming may pose a risk to inducing human corneal inflammation.

Key words： plateau city indoor dust heavy metals human corneal epithelial cells inflammatory reaction

Received: 15 December 2020

PACS:	X513
	X503.1

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	Articles by authors
	BI Jue
	ZHANG Zhen-ning
	LIU Yu-jia
	YANG Dan-lei
	ZHENG Qin-xiang
	CUI Dao-lei
	XIANG Ping

Cite this article:

BI Jue,ZHANG Zhen-ning,LIU Yu-jia等. Damage to human corneal epithelial cells caused by indoor dust in plateau city[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(7): 3360-3370.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I7/3360

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