微生物诱导碳酸盐沉积介导的Cd<sup>2+</sup>固定

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Abstract Under laboratory culture conditions, three carbonate mineralization bacteria, Pseudomonas stutzeri, Bacillus subtilis and Bacillus pasteurii were used to study the growth specialities, the resistance and removal of Cd²⁺ by the bacteria, and the effect of hydroxyapatite on Cd²⁺ immobilization. The scanning electron microscope (SEM), energy dispersive spectrum (EDS), fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) were employed to characterize the biomineralization products. The results showed that the urease activity of B. pasteurii was about 10 times than those of P.stutzeri and B.subtilis. The CO₃^2- was produced and the pH of culture media had an increasing during the growth of the three bacteria. The highest CO₃^2-concentrations were 588.19, 661.72, 1735.18mg/L, while the highest pH value could reach 8.23, 9.06 and 9.52 for P. stutzeri, B. subtilis and B. pasteurii, respectively. The growth of P. stutzeri was not affected within the Cd²⁺ concentration of 0~10mg/L, while the growth of B. subtilis and B. pasteurii were inhibited in the Cd²⁺ concentration beyond 1mg/L. With the initial Cd²⁺ concentration of 0.5mg/L and biomineralization for 120h, the removal rates of Cd²⁺ by P. stutzeri, B. subtilis and B. pasteurii were 96.37%, 99.40% and 97.57%, respectively. The addition of hydroxyapatite could enhance the immobilization of Cd²⁺by the bacteria to a certain extent. SEM and EDS analysis showed that the mineralized products induced by P. stutzeri and B. subtilis were mostly clustered around or attached to the surface of the bacteria, with spherical, reticulated structure and porous surface, while the mineralized products by B. pasteurii were attached to the surface of the bacteria, with compact structure and irregular spherical structure. FTIR analysis suggested that there was CO₃^2- in the mineralized products. XRD identified, the mineralized products CaCO₃ induced by the three bacteria. The CdCO₃ crystal were forms by isomorphic substitution of Ca²⁺ with Cd²⁺. As an alternative, Ca_0.67Cd_0.33CO₃ co-precipitation were induced by B. pasteurii for Cd²⁺ immobilization.

Key words： microbiological mineralization cadmium carbonate precipitation hydroxyapatite

Received: 09 April 2021

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X172

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	ZHU Guang-sen
	WANG Wen-jun
	WU Zheng
	SI You-bin

Cite this article:

ZHU Guang-sen,WANG Wen-jun,WU Zheng等. Immobilization of Cd²⁺ by microbial induced carbonate precipitation[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(12): 5912-5920.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I12/5912

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