可培养细菌多样性及抗辐射-抗氧化相关性特征——以库姆塔格沙漠东缘为例

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1911 KB) HTML (0 KB)
Export: BibTeX | EndNote (RIS)

Abstract This study investigated the phylogenetic diversity of culturable bacteria in the eastern edge of Kumtag Desert, and then screened the radiation-resistant and oxidant-resistant strains via their survival rate after radiation and oxidation treatments. The correlations between their survival rates under the two treatments were also used to find the coupling relationship between the two capacities of bacteria. The results showed that pH, TOC, and TN were the key factors that influenced the community structure of culturable bacteria in the desert environment. In total, 105 bacteria strains were isolated from the eastern edge of the Kumtag Desert affiliated to the phyla of Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. Actinobacteria was the most dominant phylum in culturable bacteria in both abundance (9.96×10⁵CFU/g) and species richness (72 strains from 9 genera). The culturable bacterial strains from the phyla Actinobacteria, Firmicutes, and Proteobacteria, were found to have both two capabilities to resist radiation and oxidation, but not bacterial strains from the phylum Bacteroidetes. The strains with D₁₀ dose (with survival rate greater than 10%) were higher than 100J/m² of UV-C radiation and accounted for 21.3% of the total culturable bacteria. The proportion of the anti-oxidant strains with D₁₀ dose higher than 10mmol/L H₂O₂ accounted for 70.2%. The study showed that the two capabilities of culturable bacteria have positive correlation characteristics. Three strains from the actinobacteria, K4-10, K5-3, and K2-40, have very high capabilities to resist both radiation and oxidation.

Key words： Kumtag Desert culturable bacteria diversity radiation-resistance anti-oxidation relative correlation extreme environment

Received: 12 May 2021

PACS:

X172

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LIU Yang
	WANG Xin-yue
	CHEN Tuo
	ZHANG Gao-sen
	WU Fa-si
	ZHANG Wei
	LIU Guang-xiu
	WU Yu-jie
	XU Ye-teng
	TIAN Mao

Cite this article:

LIU Yang,WANG Xin-yue,CHEN Tuo等. The diversity of culturable bacteria in the eastern edge of Kumtag Desert, and the characteristic of their radiation-resistance and anti-oxidation, including the correlation and coupling of these capacities[J]. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(12): 5921-5932.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2021/V41/I12/5921

[1]	Becherucci M E, Alvarez M F, Iribarne O, et al. Eutrophication in a semi-desert coastal ecosystem promotes increases in N and C isotopic signatures and changes in primary sources[J]. Marine Environmental Research, 2019,146:71-79.
[2]	Santiago I F, Goncalves V N, Gómez-silva B, et al. Fungal diversity in the Atacama Desert[J]. Antonie van Leeuwenhoek, 2018,111(8):1345- 1360.
[3]	Gu D, Otieno D, Huang Y, et al. Higher assimilation than respiration sensitivity to drought for a desert ecosystem in Central Asia[J]. The Science of the Total Environment, 2017,609:1200-1207.
[4]	Wang X, Chen F, Hasi E, et al. Desertification in China: An assessment[J]. 2008,88(3/4):188-206.
[5]	Cowan D A, Hopkins D W, Jones B E, et al. Microbiomics of Namib Desert habitats[J]. Extremophiles: Life Under Extreme Conditions, 2020,24(1):17-29.
[6]	Eida A A, ZieglerR M, Lafi F F, et al. Desert plant bacteria reveal host influence and beneficial plant growth properties[J]. PloS one, 2018,13(12):208-223.
[7]	Meng F, Bar-Shmuel N, Shavit R, et al. Gut bacteria of weevils developing on plant roots under extreme desert conditions[J]. BMC microbiology, 2019,19(1):311.
[8]	Perera I, Subashchandrabose S R, Venkateswarlu K, et al. Consortia of cyanobacteria/microalgae and bacteria in desert soils: an underexplored microbiota[J]. Applied Microbiology and Biotechnology, 2018,102(17): 7351-7363.
[9]	刘光琇,陈拓,李师翁,等.极端环境微生物学[M]. 北京:科学出版社, 2016:2-18. Liu G X, Chen T, Li S W, et al. Extreme environmental microbiology[M]. Beijing:Science Press., 2016:2-18.
[10]	Davila A F, B Gómez-Silva, Rios Adl, et al. Facilitation of endolithic microbial survival in the hyperarid core of the Atacama Desert by mineral deliquescence[J]. Journal of Geophysical Research, 2015,113: 1-9.
[11]	Mckay C P, Friedmann E I, Gomez-Silva B, et al. Temperature and moisture conditions for life in the extreme arid region of the Atacama desert: four years of observations including the El Nio of 1997~1998[J]. Astrobiology, 2003,3(2):393-406.
[12]	Hezbri K, Ghodhbane-Gtari F, Montero-Calasanz M D C, et al. Geodermatophilus pulveris sp. nov., a gamma-radiation-resistant actinobacterium isolated from the Sahara desert[J]. International Journal of Systematic and Evolutionary Microbiology, 2016,66(10): 3828-3834.
[13]	Hezbri K, Ghodhbane-Gtari F, Montero-Calasanz M D C, et al. Geodermatophilus sabuli sp. nov., a γ-radiation-resistant actinobacterium isolated from desert limestone[J]. International Journal of Systematic and Evolutionary Microbiology, 2015,65(10): 3365-3372.
[14]	Paulino-Lima I G, Fujishima K, Navarrete J U, et al. Extremely high UV-C radiation resistant microorganisms from desert environments with different manganese concentrations[J]. Journal of Photochemistry and Photobiology B, Biology, 2016,163:327-336.
[15]	Paulino-Lima I G, Azua-Bustos A, Vicuña R, et al. Isolation of UVC-tolerant bacteria from the hyperarid Atacama Desert, Chile[J]. Microbial Ecology, 2013,65(2):325-335.
[16]	Yu L Z, Luo X S, Liu M, et al. Diversity of ionizing radiation-resistant bacteria obtained from the Taklimakan Desert[J]. Journal of Basic Microbiology, 2015,55(1):135-40.
[17]	Li J, Zhang B, Liu G, et al. Radiobacillus deserti gen. nov., sp. nov., a UV-resistant bacterium isolated from desert soil[J]. International Journal of Systematic and Evolutionary Microbiology, 2020,70(12): 6338-6347.
[18]	Georgiou C D, Sun H J, Mckay C P, et al. Evidence for photochemical production of reactive oxygen species in desert soils[J]. Nature communications, 2015,6(1):7100.
[19]	Luo X, Zeng X C, He Z, et al. Isolation and characterization of a radiation-resistant bacterium from Taklamakan Desert showing potent ability to accumulate Lead (II) and considerable potential for bioremediation of radioactive wastes[J]. Ecotoxicology (London, England), 2014,23(10):1915-1921.
[20]	Wang G, Deng S, Li C, et al. Damage to DNA caused by UV-B radiation in the desert cyanobacterium Scytonema javanicum and the effects of exogenous chemicals on the process[J]. Chemosphere, 2012,88(4):413-417.
[21]	Li S, Lei X, Qin L, et al. Fe(III) reduction due to low pe+pH contributes to reducing Cd transfer within a soil-rice system[J]. Journal of Hazardous Materials, 2021,415:125668.
[22]	Zhao K, Yang D, Zeng P, et al. Effect of water content on the failure pattern and acoustic emission characteristics of red sandstone[J]. International Journal of Rock Mechanics and Mining Sciences, 2021,142:104709.
[23]	鲍士旦.土壤农化分析[M]. 北京:中国农业出版社, 2011:18-36. Bao S D. Agricultural chemical analysis of soil[M]. Beijing: China Agricultural Press, 2011:18-36.
[24]	李婷,张威,刘光琇,等.荒漠土壤微生物群落结构特征研究进展[J]. 中国沙漠, 2018,38(2):329-338. Li T, Zhang W, Liu G X, et al. Advances in the study of microbial ecology in desert soil[J]. Journal of Desert Research, 2018,38(2):329- 338.
[25]	李婷,张威,吴明辉,等.荒漠土壤中两株抗氧化细菌的抗氧化生理生化特征[J]. 微生物学通报, 2020,47(2):379-389. Li T, Zhang W, Wu M H, et al. Physiological and biochemical characteristics of two antioxidant bacteria in desert soil[J]. Microbiology China, 2020,47(2):379-389.
[26]	Mandakovic, D, Maldonado, J, Pulgar, R, et al. Microbiome analysis and bacterial isolation from Lejía Lake soil in Atacama Desert[J]. Extremophiles: Life Under Extreme Conditions, 2018,22(4):665-673.
[27]	Arocha-Garza H F, Canales-Del Castillo R, Eguiarte L E, et al. High diversity and suggested endemicity of culturable Actinobacteria in an extremely oligotrophic desert oasis[J]. PeerJ, 2017,5:e3247.
[28]	Contador C A, Veas-castillo L, Tapia E, et al. Atacama database: a platform of the microbiome of the Atacama Desert[J]. Antonie van Leeuwenhoek, 2020,113(2):185-195.
[29]	Okoro C K, Brown R, Jones A L, et al. Diversity of culturable actinomycetes in hyper-arid soils of the Atacama Desert, Chile[J]. Antonie van Leeuwenhoek, 2009,95(2):121-133.
[30]	Molina-menor E, Gimeno-valero H, Pascual J, et al. High culturable bacterial diversity from a European Desert: The Tabernas Desert[J]. Frontiers in Microbiology, 2020,11:583120.
[31]	刘阳.塔克拉玛干沙漠可培养微生物多样性及抗辐射—抗氧化代谢机制研究[D]. 兰州:西北师范大学, 2020:36-57. Liu Y. Diversity of culturable microorganisms and mechanisms of radiation-antioxidant metabolism in taklimakan Desert[D]. Lanzhou: Northwest Normal University, 2020:36-57.
[32]	张振清,张昺林,张威,等.河西走廊黑戈壁生态系统中可培养细菌分布特征及抗辐射活性[J]. 中国沙漠, 2020,40(4):52-62. Zhang Z Q, Zhang B L, Zhang W, et al. Distribution characteristics and anti-radiation activity of culturable bacteria in black gobi ecosystem of the Hexi Corridor[J]. Journal of Desert Research, 2020, 40(4):52-62.
[33]	吴明辉,章高森,陈拓,等.石生微生物研究进展[J]. 微生物学杂志, 2017,37(4):64-73. Wu M H, Zhang G S, Chen T, et al. Advance in lithophilous microorganisms[J]. Journal of Microbiology, 2017,37(4):64-73.
[34]	田茂,张威,章高森,等.珠穆朗玛峰北坡可培养石生细菌多样性及抗紫外辐射能力[J]. 微生物学通报, 2021,48(6):1907-1919. Tian M, Zhang W, Zhang G S, et al. Diversity and ability of resistance to ultraviolet radiation of culturable lithophilous bacteria on the north slope of Mount Everest[J]. Microbiology China, 2021,48(6):1907- 1919.
[35]	Zhang B, Wu X, Zhang G, et al. The diversity and biogeography of the communities of Actinobacteria in the forelands of glaciers at a continental scale[J]. Environmental Research Letters, 2016,11: 054012.
[36]	Zhang X, Xu S, Li C, et al. The soil carbon/nitrogen ratio and moisture affect microbial community structures in alkaline permafrost-affected soils with different vegetation types on the Tibetan plateau[J]. Research in Microbiology, 2014,165(2):128-139.
[37]	Guo H, Ma L, Liang Y, et al. Response of ammonia-oxidizing Bacteria and Archaea to long-term saline water irrigation in alluvial grey desert soils[J]. Scientific Reports, 2020,10(1):489.
[38]	Jordaan K, Lappan R, Dong X, et al. Hydrogen-oxidizing bacteria are abundant in desert soils and strongly stimulated by hydration[J]. Antimicrobial Agents and Chemotherapy, 2020,5(6):1120-1131.
[39]	Robinson C K, Wierzchos J, Black C, et al. Microbial diversity and the presence of algae in halite endolithic communities are correlated to atmospheric moisture in the hyper-arid zone of the Atacama Desert[J]. Environmental Microbiology, 2015,17(2):299-315.
[40]	张威,章高森,刘光琇,等.腾格里沙漠东南缘可培养微生物群落数量与结构特征[J]. 生态学报, 2012,32(2):567-577. Zhang W, Zhang G S, Liu G X, et al. Characteristics of cultivable microbial community number and structure at the southeast edge of Tengger Desert[J]. Acta Ecologica Sinica, 2012,32(2):567-577.
[41]	Cheng Y, ZhangH, Chen Z, et al. Contrasting effects of different pH‐raising materials on N2O emissions in acidic upland soils[J]. European Journal of Soil Science, 2020,72(1):432-445.
[42]	Wu J, Pang H, Zhuo Y, et al. Effects of vegetation degradation on carbon and nitrogen distribution of roots-soil system in temperate typical steppe[J]. Acta Ecologica Sinica, 2018,38(15):5340-5350.
[43]	Faoro H, Alves A C, Souza E M, et al. Influence of soil characteristics on the diversity of bacteria in the Southern Brazilian Atlantic Forest[J]. Applied and Environmental Microbiology, 2010,76(14):4744-4749.
[44]	Rainey F A, Ray K, Ferreira M, et al. Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus obtained from a single soil sample[J]. Applied and Environmental Microbiology, 2005,71(9):5225-5235.
[45]	Jin M, Xiao A, Zhu L, et al. The diversity and commonalities of the radiation-resistance mechanisms of Deinococcus and its up-to-date applications[J]. AMB Express, 2019,9(1):138.
[46]	Baqué, M, Viaggiu E, Scalzi G, et al. Endurance of the endolithic desert cyanobacterium Chroococcidiopsis under UVC radiation[J]. Extremophiles: Life Under Extreme Conditions, 2013,17(1):161-169.