基于定位栏试验的奶牛粪便中雌激素含量特征

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Abstract To explore characteristics of estrogen content in cow feces, fecal samples were collected from the gestation crates to quantify the contents of 4 natural estrogens (including estrone, 17α-estradiol, 17β-estradiol, estriol) and 2 synthetic estrogens (17α-ethinylestradiol, diethylstilbestrol) with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The results showed that the detection frequency (DF) of 6 estrogens ranged from 8.33% to 98.89%. The DF of detected natural estrogens was higher than 90%, except for estriol (8.33%); while the DF of synthetic estrogens were 52.22% and 64.44% for 17α-estradiol and diethylstilbestrol, respectively. The total content of 6 estrogens was in the range of 1.15~658.91μg/kg (dry weight, dw). The total estrogenic activity was equivalent to the content of 3.76~478.50μg/kg (dw). A significant difference in the total content (median) of 6 estrogens among different growth stages was observed in the order of: lactating cows (79.21μg/kg)>calves (47.20μg/kg)>heifers (25.72μg/kg). The content of estrogens in fecal samples demonstrate large seasonal variations: relatively higher in winter and spring than in summer and autumn andthe lowest concentration of 6 estrogens was observed in autumn. The total concentration of 6 estrogens in the feces collected from fecal stock dump ranged from 7.63 to 3921.92μg/kg which were significantly higher than that in fresh feces from gestation crate. Clearly, cow growth stage, sampling time (season) and sampling sites influenced the content of estrogens in cow feces, which should be fully considered in the investigation and risk assessment of estrogen emissions for cow breeding.

Key words： dairy cows feces estrogen growth stage seasonal variation

Received: 31 May 2021

PACS:	X713
	171.5

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	LI Yan
	PAN Jie
	LIANG Zi-wei
	HU Jia-wu
	LIU Jiang-yan
	HE De-chun
	LI Ting-zhen

Cite this article:

LI Yan,PAN Jie,LIANG Zi-wei等. Characterizing the estrogen content in cow feces based on gestation crate experiment[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(1): 119-126.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I1/119

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