蚯蚓摄食污泥对其肠道功能区微生物种群及耐药基因的影响

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Abstract This study aimed to investigate the changes of microbial community structure and their antibiotics resistance genes (ARGs) abundances in the intestinal tract of Eisenia fetida, via detecting the sludge passing through different functional areas in earthworms' gut. Prior to the DNA extraction, all fresh samples were pretreated with propidium monoazide (PMA) to characterize active functional microorganisms. The results showed that the abundances of bacterial 16S rDNA and eukaryotic 18S rDNA significantly increased (P<0.05) by 28.2 and 42.2 times in stomach, and they significantly decreased (P<0.05) in the gizzard and hindgut, after 5 days of earthworm digestion. In addition, the sludge digested by earthworms changed dominant proteobacteria to tenericutes in gizzard, and modified dominant bacteroidetes to firmicutes in stomach. However, the digestion process of earthworms had little effect on bacterial community structure in hindgut. For the ARGs, the abundances of ermF, tetX and sul2 significantly increased (P<0.05) by 1.9×10², 8.4×10⁵ and 25.9 times in stomach of earthworm, respectively. While the total abundances of ARGs in gizzard and hindgut significantly decreased (P<0.05) by 11.0 and 45.2 times, respectively. Moreover, network analysis revealed that the feeding behavior of earthworms for sludge could indicate effects on the structural diversity of host bacteria of ARGs in their intestine.

Key words： sludge recycling vermicomposting resistance genes gut microbiota biological pollutants

Received: 25 May 2021

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X172

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	PENG Lan-sheng
	GUAN Meng-xin
	HUANG Kui
	XIA Hui
	SANG Chun-lei

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PENG Lan-sheng,GUAN Meng-xin,HUANG Kui等. Effects of excess sludge fed by earthworms on microbial community and antibiotic resistance genes in their intestinal functional area[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(1): 465-473.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I1/465

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