微生物群落对氨胁迫响应的宏基因组学研究

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Abstract A series of batch experiments were conducted to investigate the responses of anaerobic digestion (AD) microbial community exposed to ammonia (TAN) stress. In combination with Metagenomic sequencing analysis, this paper investigated the responses of process parameters, community structure/composition and functional gene/metabolic pathways of AD to the process disturbance induced by different TAN concentrations. The overall performance of AD system was gradually optimized at the concentration of 3000mg/L TAN while when TAN reached 6000mg/L, an accumulation of propionate and butyrate was observed with a concomitant decrease of methane yield. At both concentration levels, the resistance and redundancy of hydrolytic and acidogenic bacteria can ensure their metabolic performance for substrate degradation. However, the total relative abundance of acetogens and methanogens at the low concentration level continued to increase, whereas a low increase rate and even a decrease in the total relative abundance was observed at the high level, suggesting the potential process inhibition. By further analyzing the functional gene changes in the two process stages, The methanogenesis and syntrophic acetate oxidation pathways showed resistance and redundancy under the ammonia stress, but the high concentration of TAN (6000mg/L) inhibited the expression of key genes ACADS and PCCA in the syntrophic degradation of butyrate and propionate. Therefore, the key contributor to the ammonia inhibition turned out to be acetogenesis.

Key words： food waste anaerobic digestion ammonia stress metagenomics

Received: 22 June 2021

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X705

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	PENG Yun
	LI Lei
	WU Di
	YANG Ping-jin
	PENG Xu-ya
	WANG Xiao-ming

Cite this article:

PENG Yun,LI Lei,WU Di等. Metagenomic analysis on the responses of microbial community to ammonia stress[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(2): 777-786.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I2/777

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