有氧和厌氧环境下指示微生物的稳定性特征

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Abstract We investigated the variations of pig-associated genetic markers, several microbiological indicators and potential pathogens in anaerobic and aerobic treatments using qPCR and Illumina sequencing methods. The six indicators (Streptococcaceae、Lactobacillaceae、Carnobacteriaceae、Ruminococcaceae、Lachnospiraceae and Prevotellaceae) had a similar decay trend in anaerobic and aerobic treatments, but these taxa persisted longer in anaerobic than aerobic treatments. Hence, the six indicators all can be used for identifying the recent pollution issues in aerobic water environment. The genetic markers including GenBac and Pig-2-Bac showed biphasic decay model in both aerobic and anaerobic treatments, and the first decay stage contributed the majority decay. However, the decay rates of genetic markers were significantly higher in aerobic than anaerobic treatments, which suggests the poor stability of the markers in aerobic water environment. For the identified potential pathogens, the Bacteroidales genetic markers presented higher correlation with Prevotella, Pseudomonas and Erysipelothrix(P<0.05), but only Prevotella showed similar correlation coefficient in both anaerobic and aerobic treatments (0.913~0.953), which demonstrate the usefulness of the Bacteroidales genetic markers for predicting the presence of Prevotella.

Key words： fecal pollution microbial source tracking(MST) genetic markers indicators stability

Received: 09 August 2021

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X172

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	Articles by authors
	ZHANG Yang
	ZHENG Qian-xing
	WU Ren-ren
	LI Guo-dong
	ZHONG Yi
	CHEN Zhong-ying
	WEI Si-ye
	LI Kai-ming

Cite this article:

ZHANG Yang,ZHENG Qian-xing,WU Ren-ren等. The stability of microbiological indicators in aerobic and anaerobic environment[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(3): 1327-1334.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I3/1327

[1]	Wéry N, Monteil C, Pourcher A M, et al. Human-specific fecal bacteria in wastewater treatment plant effluents[J]. Water Research, 2009,44(6):1873-1883.
[2]	Wu B L, Wang X C, Dzakpasu M. Genetic characterization of fecal impacts of seagull migration on an urban scenery lake[J]. Water Research, 2017,117:27-36.
[3]	Emma R N, Nguyen T M H, Le T P Q, et al. A short review of fecal indicator bacteria in tropical aquatic ecosystems:knowledge gaps and future directions[J]. Frontiers in Microbiology, 2015,6:308.
[4]	Winfield M D, Groisman E A. Role of nonhost environments in the lifestyles of Salmonella and Escherichia coli [J]. Applied and Environmental Microbiology, 2003,69(7):3687-3694.
[5]	Ahmed W, Gyawali P, Feng S, et al. Host-specificity and sensitivity of the established and novel sewage-associated marker genes in human and non-human fecal samples[J]. Applied and Environmental Microbiology, 2019,85(14):e00641-19.
[6]	Hagedorn C, Blanch A R, Harwood V J. Microbial source tracking:methods, applications, and case Studies[M]. New York:Springer, NY, 2011:301-312.
[7]	Harwood V J, Staley C, Badgley B D, et al. Microbial source tracking markers for detection of fecal contamination in environmental waters:relationships between pathogens and human health outcomes[J]. Fems Microbiology Reviews, 2014,38(1):1-40.
[8]	Zhang Y, Wu R R, Lin K R, et al. Performance of host-associated genetic markers for microbial source tracking in China[J]. Water Research, 2020,175:115670.
[9]	Zhang Y, Wu R R, Zhang Y M, et al. Impact of nutrient addition on diversity and fate of fecal bacteria[J]. Science of the Total Environment, 2018,636:717-726.
[10]	Brooks Y M, Spirito C M, Bae J S, et al. Fecal indicator bacteria, fecal source tracking markers, and pathogens detected in two Hudson River tributaries[J]. Water Research, 2020,17:115342.
[11]	Staley C, Kaiser T, Lobos A, et al. Application of SourceTracker for accurate identification of fecal pollution in recreational freshwater:a double-blinded study[J]. Environmental Science and Technology, 2018,52:4207-4217.
[12]	Brown C M, Staley C, Wang P, et al. A high-throughput DNA-sequencing approach for determining sources of fecal bacteria in a lake Superior estuary[J]. Environmental Science and Technology, 2017,51(15):8263-8271.
[13]	Warish A, Christopher S, Thomas K, et al. Decay of sewage-associated bacterial communities in fresh and marine environmental waters and sediment[J]. Applied Microbiology and Biotechnology, 2018,102:1-12.
[14]	Rhodes J, Hetzenauer H, Frassl M A, et al. Long-term development of hypolimnetic oxygen depletion rates in the large Lake Constance[J]. Ambio., 2017,46:554-565.
[15]	Marti R, Mieszkin S, Solecki O, et al. Effect of oxygen and temperature on the dynamic of the dominant bacterial populations of pig manure and on the persistence of pig-associated genetic markers, assessed in river water microcosms[J]. Journal of Applied Microbiology, 2011,111(5):1159-1175.
[16]	Gao C, Wang A, Wu W M, et al. Enrichment of anodic biofilm inoculated with anaerobic or aerobic sludge in single chambered air-cathode microbial fuel cells[J]. Bioresource Technology, 2014,167:124-132.
[17]	Sokolova E, Aström J, Pettersson T, et al. Decay of Bacteroidales genetic markers in relation to traditional fecal indicators for water quality modeling of drinking water sources[J]. Environmental Science and Technology, 2011,46(2):892-900.
[18]	Griffin D W, Lipp E K, Mclaughlin M R, et al. Marine recreation and public health microbiology:quest for the ideal indicator[J]. BioScience, 2001,(10):817-825.
[19]	Savichtcheva O, Okayama N, Okabe S. Relationships between Bacteroides 16S rRNA genetic markers and presence of bacterial enteric pathogens and conventional fecal indicators[J]. Water Research, 2007,41(16):3615-3628.
[20]	Boehm A B, Soller J A, Shanks O C. Human-associated fecal quantitative polymerase chain reaction measurements and simulated risk of gastrointestinal illness in recreational waters contaminated with raw sewage[J]. Environmental Science and Technology Letters, 2015,2(10):270-275.
[21]	Nshimyimana J P, Freedman A, Shanahan P, et al. Variation of bacterial communities with water quality in an urban tropical catchment[J]. Environmental Science and Technology, 2017,51(10):5591-5601.
[22]	Siefring S, Varma M, Atikovic E, et al. Improved real-time PCR assays for the detection of fecal indicator bacteria in surface waters with different instrument and reagent systems[J]. Journal of Water and Health, 2008,6(2):225-237.
[23]	Mieszkin S, Furet J P, Corthier G, et al. Estimation of pig fecal contamination in a river catchment by real-time PCR using two pig-specific Bacteroidales 16S rRNA genetic markers[J]. Applied and Environmental Microbiology, 2008,75(10):3045-3054.
[24]	Chern E C, Siefring S, Paar J, et al. Comparison of quantitative PCR assays for Escherichia coli targeting ribosomal RNA and single copy genes[J]. Letters in Applied Microbiology, 2011,52(3):298-306.
[25]	Ahmed W, Zhang Q, Kozak S, et al. Comparative decay of sewage-associated marker genes in beach water and sediment in a subtropical region[J]. Water Research, 2019,149:511-521.
[26]	Jeanneau L, Solecki O, Wery N, et al. Relative decay of fecal indicator bacteria and human-associated markers:A microcosm study simulating wastewater input into seawater and freshwater[J]. Environmental Science and Technology, 2012,46(4):2375-2382.
[27]	Zhang Y, Wu R R, Li W J, et al. Occurrence and distributions of human-associated markers in an impacted urban watershed[J]. Environmental Pollution, 2021,275(14):116654.
[28]	Mantilla-Calderon D, Hong P Y. Fate and persistence of a pathogenic NDM-1-positive Escherichia coli strain in anaerobic and aerobic sludge microcosms[J]. Applied and Environmental Microbiology, 2017,83(13):e00640-17.
[29]	Liu R L, Cheng K H F, Wong K, et al. Differential utility of the Bacteroidales DNA and RNA markers in the tiered approach for microbial source tracking in subtropical seawater[J]. Applied Microbiology and Biotechnology, 2015,99(13):5669-5681.
[30]	Easton J H, Gauthier J J, Lalor M M, et al. Die-soff pathogenic E.coli O157:H7 in sewage contaminated waters[J]. Jawra Journal of the American Water Resources Association, 2010,41(5):1187-1193.
[31]	Rogers S W, Donnelly M, Peed L, et al. Decay of bacterial pathogens, fecal indicators, and real-time quantitative PCR genetic markers in manure-amended soils[J]. Applied and Environmental Microbiology, 2011,77(14):4839-4848.
[32]	Strauss E. Microbes, immunity, and disease:a symphony of bacterial voices[J]. Science, 1999,284(5418):1302-1304.
[33]	Pachepsky Y, Kierzewski R, Stocker M, et al.Temporal stability of Escherichia coli concentrations in waters of two irrigation ponds in Maryland[J]. Applied and Environmental Microbiology, 2018,84(3):e01876-17.
[34]	Dwivedi D, Mohanty B P, Lesikar B J. Impact of the linked surface water-soil water-groundwater system on transport of E. coli in the subsurface[J]. Water Air and Soil Pollution, 2016,227(9):351.
[35]	王文文,王园,安晓萍,等.断奶仔猪肠道菌群结构特征及其调控研究进展[J].动物营养学报, 2020,32(11):4998-5005. Wang W W, Wang Y, An X P, et al. Research advances on structural characteristics and modulation of gut microbiota of weaned piglets[J]. Chinese Journal of Animal Nutrition, 2020,32(11):4998-5005.
[36]	郑青.不同饲养模式对猪肠道菌群结构的影响研究[D].合肥:安徽农业大学, 2017. Zheng Q. The effects of different breeding patterns on intestinal microflora structure in pigs[D]. Hefei:Anhui Agricultural University, 2017.
[37]	Eichmiller J J, Borchert A J, Sadowsky M J, et al. Decay of genetic markers for fecal bacterial indicators and pathogens in sand from Lake Superior[J]. Water Research, 2014,59:99-111.
[38]	Wattam A R, David A, Dalay O, et al. PATRIC, the bacterial bioinformatics database and analysis resource[J]. Nucleic Acids Research, 2014,42(D1):D581-D591.
[39]	Cui Q J, Fang T T, Huang Y, et al. Evaluation of bacterial pathogen diversity, abundance and health risks in urban recreational water by amplicon next-generation sequencing and quantitative PCR.[J]. Journal of environmental sciences (China), 2017,57:137-149.
[40]	Lee J Y, Kim H J, Kim E S, et al. Regulatory interaction of the Corynebacterium glutamicum whc genes in oxidative stress responses[J]. Journal of Biotechnology, 2013,168(2):149-154.
[41]	Craig K, Johnson B R, Grunden A. Leveraging Pseudomonas Stress Response Mechanisms for Industrial Applications[J]. Frontiers in Microbiology, 2021,12:660134.
[42]	Alfei S, Caviglia D, Piatti G, et al. Bactericidal activity of a self-biodegradable lysine-containing dendrimer against clinical isolates of Acinetobacter genus[J]. International Journal of Molecular Sciences, 2021,22(14):7274.
[43]	肖晶,郭丽英,赵永,等.一株高效除磷的不动杆菌根瘤菌及其应用:中国, 201910185330.5[P]. 2019-05-17. Xiao J, Guo L Y, Zhao Y, et al. An efficient phosphorus removal strain of Acinetobacter rhizosphaerae and its application:China, 201910185330.5[P]. 2019-05-17.