三氯生对斑马鱼发育和脂质代谢的影响

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Abstract Herein, we explored in detail the effects of TCS acute exposure at sublethal dosage on zebrafish embryonic development and lipid metabolism. TCS at 200µg/L significantly delayed the 72-hpf embryonic hatching with a decreased hatching percentage of 13.24% and an increased deformity rate of >80%. In the TCS-exposure group (4-120hpf), the main larval malformations were reflected in energy stasis、yolk cyst、dyssorption and swim sac closure, pericardial cyst, spinal curvature and so on. Using metascape online database, the target molecules of TCS, and the underlying GO (gene ontology) function clustering and KEGG (kyoto encyclopedia of genes and genomes) metabolic pathways were analyzed systemically. Most of the target genes were involved in various biological functions, such as carboxylate metabolism, steroid hormone receptor activity, lipid binding, lipid catabolic process, lipid homeostasis and regulation of lipid localization based on the roles in molecular function, biological process and cellular component. Three KEGG signaling pathways were found to be directly or indirectly concerned with lipid metabolism. The changes in biochemical indicators of 120-hpf larvae demonstrated that TCS exposure resulted in the significant increases of the total cholesterol (T-CHO) and triglycerides (TG) levels. By oil red O staining, we observed the prominent accumulation of lipid droplets in larval liver, egg yolk and blood vessels after exposure to TCS, especially in the liver and yolk. Lipid metabolism and energy supply are closely related to motor behavior. Using Noldus behavior analyzer, after TCS exposure, zebrafish voluntary motor activity was obviously inhibited, and the sensitivity to acoustic-optic stimulation was significantly decreased. The aforementioned toxic effects could be alleviated upon addition of 100µg/L sodium deoxycholate. These findings provide compelling evidence that low-dose TCS exposure induced zebrafish lipid-metabolism disorders by affecting hepatobiliary circulation and causing energy-supply obstacle.

Key words： triclosan zebrafish lipid metabolism movement behavior sodium deoxycholate

Received: 30 July 2021

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X171.5

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	WANG Yang
	WU Guo-hui
	QIAN Qiu-hui
	ZHAO Chen-xi
	YAN Jin
	WANG Xue-dong
	WANG Hui-li

Cite this article:

WANG Yang,WU Guo-hui,QIAN Qiu-hui等. Effects of triclosan environmental exposure on zebrafish development and lipid metabolism[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(3): 1394-1400.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I3/1394

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