氯/氯胺和紫外顺序消毒对管网抗性基因的去除

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Abstract In order to explore the influence of chlorine/chloramine and low-pressure ultraviolet sequential disinfection on the distribution characteristics of antibiotic resistance genes (ARGs) in the water supply system, a biofilm reactor was used to simulate the water supply network. The effluent and biofilm of the pipe network were taken for 60mJ/cm² low-pressure ultraviolet (254nm) disinfection, and high-throughput quantitative PCR was used to detect the number of typical ARGs and mobile genetic elements (MGEs) in the inlet, outlet, and biofilm of the simulated pipe network. After 150days, the total relative abundance of ARGs in the effluent of chlorine and chloramine pipe network were 0.130 and 0.137, and the abundance of biofilm were 2.45 and 0.277, respectively. This indicated that low dose of chlorine or chloramine in the water supply pipe network could effectively reduce the relative abundance of ARGs in the water phase and biofilm phase by 90%, and chloramine disinfection has a more significant control effect on ARGs in biofilms. After UV disinfection, the relative abundances of ARGs in chlorine and chloramine disinfected pipe network water were 0.0682 and 0.0537, respectively. The relative abundances of ARGs in biofilms after chlorine and chloramine disinfection were 2.01 and 0.194, respectively. The correlation between ARGs and MGEs changed significantly. The correlation between the transposon and strB, mepA was enhanced, and the correlation with ermX and tetM was weakened, while the correlation between the integron and acrF, cmlA1-01, oprJ and tolC-01 was enhanced. The results showed that the ultraviolet disinfection can significantly reduce the abundance of ARGs in the chlorine and chloramine disinfected pipe network water, but it had less effect on the ARGs in the pipe network biofilms.

Key words： simulated water distribution system antibiotic resistance genes chlorination low-pressure UV high-throughput quantitative PCR

Received: 20 October 2021

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X703.5

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	WANG Yue
	BAI Miao
	JIANG Hai-rong
	ZHANG Ming-lu
	ZHANG Can

Cite this article:

WANG Yue,BAI Miao,JIANG Hai-rong等. Research on removal of antibiotic resistance genes in pipe networks by chlorine/chloramine and UV sequential disinfection[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(5): 2136-2143.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I5/2136

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