医院空调回风口滤网积尘中抗生素抗性基因污染的比较

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Abstract The objective of this study was to explore the contamination and disstrubution characteristics of antibiotic resistance genes (ARGs) in hospital environment. From November 2018 to April 2021, 127 dust samples on return vent filters from air-conditioners in different wards of 2 Wuhan hospitals were collected in multiple batches for qualitative and quantitative determination of β-lactam ARGs (mecA, bla_TEM,bla_CTX-M, bla_SHV), carbapenem ARGs (bla_KPC, bla_NDM-1, bla_IMP, bla_VIM, bla_OXA-51) and class 1integrase gene (intI1). Nine types of abovementioned ARGs and intI1were found in dust samples from different departments, with the average detection rates of 55.12%, 37.64% and 81.89% for β-lactam ARGs, carbapenem ARGs and intI1respectively. The average detection rate of β-lactam and carbapenem ARGs in ICU were significantly higher than in departments of internal medicine and surgery (P<0.05); The detection rate and relative abundance of bla_TEM and mecA were highest in departments of internal medicine and surgery among the 4types of β-lactam ARGs, (P<0.05); the detection rate and relative abundance of bla_SHV in ICU were higher than in other departments (P<0.05). Significantly higher detection rate and relative abundance were observed for bla_NDM-1 than for bla_KPC and bla_IMPamong the 5 types of carbapenem ARGs in departments of internal medicine and surgery. (P<0.05). There was a significant positive correlation between intI1and 9 types of ARGs (P<0.05). This study confirmed the contamination of β-lactam ARGs, carbapenem ARGs and intI1in dusts retaining on the return vent filters of air-conditioners in 2hospitals, where bla_TEM, mecA and bla_NDM-1 prevailed in ICUs and departments of internal medicine and surgery. Accordingly it was suggested that there may be past and ongoing contamination and concomitant risk of horizontal transfer of ARGs among drug-resistant bacteria, such as MRSA, ESBLs-producing Enterobacteriaceae bacteria, carbapenem-resistant Escherichia coli and Acinetobacter baumannii, in air and relevant hospital environments. The contamination of β-lactam ARGs and carbapenem ARGs in ICU was worse than in departments of internal medicine and surgery, where higher prevalence of bla_SHV was found. ICU may be the high-risk department of drug-resistant bacteria contamination.

Key words： hospital air-conditioners filter dust antibiotic resistance genes antibiotic-resistant bacteria

Received: 24 September 2022

PACS:	X172
	R122

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	QIU Wan-yue
	XIA Yu-he
	GONG Lin
	YUAN Feng-yun
	CHEN Qi
	LI Jia-hao
	LIANG Jian-sheng
	TANG Fei

Cite this article:

QIU Wan-yue,XIA Yu-he,GONG Lin等. Comparison of antibiotic resistance genes contamination in the dust on the return vent filters of air-conditioners in Wuhan hospital[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(5): 2321-2330.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I5/2321

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