不同城市PM<sub>2.5</sub>致小鼠肝脏纤维化的效应差异研究

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Abstract To clarify the potential mechanism by which PM_2.5 exposure causes liver fibrosis and the key components leading to this adverse effect, 10-month-old C57BL/6 female mice were exposed to PM_2.5 from different cities of China (Taiyuan, Beijing, Hangzhou and Guangzhou) for 4weeks. And tissue section staining, qRT-PCR and Western-blot technique were used to evaluate the features of liver fibrosis in mice. Pearson correlation coefficient was used to analyse the correlation between the expression level of liver fibrosis-related genes in mice and the typical chemical composition of PM_2.5 from different cities. The results showed that a significant decrease in the organ coefficient of liver was found in Taiyuan and Hangzhou PM_2.5-exposed group. Taiyuan PM_2.5 exposure significantly increased the area of collagen deposition and the relative expression level of fibrosis related genes (Col1a1, Col3a1, TGF-β and MMP2) in the mice liver, and only PM_2.5 from Taiyuan induced the significant increase of Col1a1 protein in mice liver, while PM_2.5 from other cities except Taiyuan exposure did not cause any of the above changes in fibrosis related factors. In addition, the results of correlation analysis showed that the metal components such as Cr, Mn, Mo, Cs, Pb, Bi, U and Fe were positively correlated with the mRNA expression of Col1a1 and Col3a1. Except for NA, AC and BaP, the other 15PAHs were significantly correlated with Col1a1 mRNA expression, and the sum of 18EPA PAHs was significantly correlated with Col3a1. The above results indicated that PM_2.5 exposure induced hepatic fibrosis in mice, especially PM_2.5 from Taiyuan. TGF-β and related signal molecules in its pathway has beenshownto play a vital role in mediating liver fibrosis induced by PM_2.5. Cr, Mn, Mo, Cs, Pb, Bi, U, Fe and PAHs of PM_2.5 were probably critical components to induce liver fibrosis.

Key words： PM_2.5 liver fibrosis critical component

Received: 03 November 2021

PACS:

X171.5

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	Articles by authors
	YAN Zhi-peng
	LI Shu-yue
	XING Qi-song
	NAN Nan
	QIN Guo-hua
	SANG Nan

Cite this article:

YAN Zhi-peng,LI Shu-yue,XING Qi-song等. Differential Effects of PM_2.5from different cities in the induction of hepaticfibrosis in mice[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(6): 2878-2885.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I6/2878

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