6:2氟调羧酸在蚯蚓体内的毒理效应及代谢转化

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Abstract Earthworms (Eisenia fetida) were exposed to the soil spiked with 6:2 fluorotelomeric carboxylic acid (6:2 FTCA) to investigate the toxicological effects and biotransformation mechanisms of 6:2 FTCA in earthworms after in vivo and in vitro exposure. Compared to the controls, no significant effects were observed in malondialdehyde (MDA) contents and peroxidase (POD) activities, while catalase (CAT) activities were increased, and superoxide dismutase (SOD) and glutathione-s-transferase (GST) activities were significantly induced in 6:2 FTCA treatments. This suggested that 6:2 FTCA induced oxidative stress in the earthworm cells. Biodegradation of 6:2 FTCA in the earthworm cytolchrome P450 (CYP450) enzyme extracts and GST enzyme extracts fitted well with the first order kinetics. The biodegradation rate in CYP450 extracts (0.014/h) was much higher than that in GST extracts (0.006/h), indicating CYP450 and GST were involved in the enzymatic transformation and CYP450 contributed more than GST to 6:2 FTCA biotrans formation in earthworms. Three terminal perfluorocarboxylic acid (PFCA) metabolites, including perfluorohexanoic acid (PFHxA), perfluoropentanoic acid (PFPeA) and perfluorobutanoic acid (PFBA) were observed in the enzyme extracts. The results of gut microbial degradation test showed that aerobic microorganisms contributed to 6:2 FTCA biodegradation in earthworms significantly, and the terminal PFCA metabolites were PFHxA and PFPeA, but anaerobic microorganisms didn't contribute to 6:2 FTCA biotransformation in earthworms.

Key words： 6:2 FTCA earthworm toxicological effects enzyme metabolism gut microorganisms

Received: 22 November 2021

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X171.5

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	ZONGYu-lu
	YANG Li-ping
	ZHAO Shu-yan

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ZONGYu-lu,YANG Li-ping,ZHAO Shu-yan. Toxicological effects and biotransformation mechanism of 6:2fluorotelomer carboxylic acid (6:2FTCA) in earthworms (Eisenia fetida)[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(6): 2886-2893.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I6/2886

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