厌氧水环境中噬菌体MS2的存活和团聚特性及理化因素的影响

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Abstract Bacteriophage MS2 was chosen as the model virus to reveal the survival characteristics of virus in anaerobic water environment. The effect of physicochemical conditions, including temperature, pH, suspended particles, and acetic acid on bacteriophage MS2 were studied by double-layer plate method. The decay kinetics of bacteriophage MS2 were analyzed, and the aggregation of phage particles under different conditions was investigated by measuring Zeta potential and plaque diameter. The results showed that the decay of bacteriophage MS2 in anaerobic water environment followed the first-order exponential decay model. Among the above-mentioned factors, temperature was the most important factor affecting the survival of bacteriophage MS2. At 4℃, 17℃, 25℃ and 35℃, the T₉₀ values of bacteriophage MS2 were 20.36d, 6.14d, 5.15d and 0.46d, respectively. The inactivation rate of bacteriophage MS2 was as high as 2.44lg after 12h at 35℃, while the inactivation rate was only 0.78lg after 7days at 4℃. Increasing the concentration of acetic acid significantly enhanced the inactivation rate of bacteriophage MS2. Low pH value and suspended particles promoted the aggregation of bacteriophage MS2, which caused Zeta potential decrease and the hydrodynamic diameter of phage particles increase, but excessively high concentrations of suspended particles affected the electrostatic interaction between particles. The aggregation of bacteriophage MS2 also increased the diameter of plaques. Under the condition of pH 6 and suspended particles at 20mg/L, the proportion of large plaques with diameter more than 1.0mm reached 45.61% and 57.74%, respectively. Clarification of the impact of various physicochemical factors on bacteriophage MS2 in anaerobic water environment can provide scientific basis for virus control in water environment.

Key words： anaerobic water environment bacteriophage MS2 physicochemical factors survival aggregation

Received: 09 December 2021

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X172

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	ZHANG Chong-miao
	ZHAO Qian
	WANG Zhen

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ZHANG Chong-miao,ZHAO Qian,WANG Zhen. Survival and aggregation characteristics of bacteriophage MS2 in anaerobic water environment and influence of physicochemical factors[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(7): 3434-3442.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I7/3434

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