基于活体微生物揭示蚯蚓对污泥耐药基因转归的影响

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Abstract To eliminate the abundances of ARGs in sludge vermicompost, this study aimed to reveal the underlying effects of earthworms on the active bacterial community structure and their ARGs involved in vermicomposting systems for sludge recycling. For this, vermicomposting with and without earthworms was set up in parallel. Moreover, the dyeing pretreatment for samples with propidium monoazide (PMA) was adopted to screen out the DNA of active bacteria. The results showed that the electrical conductivity of sludge vermicompost was significantly increased by 82.5% (P<0.05), the degradation rate of organic matter was increased by 5.2% (P<0.05). Compared with the control treatment, the abundance of Actinobacteria significantly increased by 65.6% (P<0.05), while the abundance of Firmicutes and Bacteroidetes significantly decreased by 74.7% and 34.6%, respectively. Meanwhile, vermicomposting led to the selected ARGs abundances of tetM, sul1, sul2, ermB and ermF significantly decreased by 66.5%, 82.8%, 72.8% and 77.6% (P<0.05), while the abundance of ermB significantly increased by 5.7times (P<0.05) in active bacteria, compared to the counterpart. The abundance of intI1gene in vermicompost products significantly reduced by 67.2% compared with the control treatment. The total absolute abundance of ARGs was 4.19×10¹³ copies/g, and the total removal rate of ARGs was 82.6%, 45.4% higher than that of the counterpart. This study suggests that earthworms can reduce the abundance of dominant hosts of ARGs by modifying the active microbial community structure of sludge, thus reducing the associated dissemination risks of the spread of ARGs.

Key words： composting microbial community resistance genes vermicompost sludge recycling

Received: 06 December 2021

PACS:

X171.5

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	Articles by authors
	WEI Feng-yi
	XU Jun-jie
	CHEN Jin
	LI Jian-hui
	HUANG Kui
	DONG Xi-lin
	XIA Hui

Cite this article:

WEI Feng-yi,XU Jun-jie,CHEN Jin等. Effects of earthworms on the antibiotic resistance genes of vermicompost from dewatered sludge revealed by active microbes[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(7): 3425-3433.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I7/3425

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