纳米ZnO胁迫下SBBR污染物去除性能及微生物群落响应

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Abstract In order to explore the tolerance of biofilm treatment system to Nano-ZnO, a sequencing batch biofilm reactor (SBBR) was constructed to carry out the Nano-ZnO stress test in SBBR system for determining the accumulation characteristics of Nano-ZnO on biofilm, investigating the removal performance of organic matter, nitrogen and phosphorus, and evaluating the Nano-ZnO tolerance threshold of SBBR. The responses of microbial community to Nano-ZnO were also analyzed by observing the changes of biomass, microbial activity and community structure. The results showed that low concentration (1~10mg/L) Nano-ZnO had no significant effect on the removal of COD, NH₄⁺-N and SOP in SBBR except that 5mg/L Nano-ZnO promoted the microbial metabolic rate and biological activity. While the concentration of Nano-ZnO increased to 50mg/L, the inhibition on biomass and microbial activity increased, and the removal of COD, NH₄⁺-N and SOP decreased by 26.45%, 57.83% and 43.50%, respectively. Nano-ZnO stress had the least effect on COD removal performance, but a great effect on NH₄⁺-N. The Nano-ZnO tolerance threshold of SBBR indicated by COD was 911.49mg, while that of NH₄⁺-N and SOP was 579.83mg. The diversity of microbial community in SBBR was reduced and the composition of community structure were changed under the stress of Nano-ZnO. The relative abundance of Proteobacteria and Chlorofiexi decreased from 21.09% and 7.03% to 8.00% and 2.60%, respectively, resulting in significant inhibition of NH₄⁺-N removal. The abundance of Paesciabacteria suddenly increased from 9.33% to 56.64%, which played an important role in the efficient removal of organic matter. The pollutant removal performance and microbial activity showed that the tolerance of SBBR biofilm system to Nano-ZnO was stronger than that of activated sludge process.

Key words： SBBR Nano-ZnO tolerance threshold high-throughput sequencing community structure

Received: 17 January 2022

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X703.1

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	GAO Jing-tian
	HU Peng
	CAI Yi-ting
	LI Wei-ping
	LIU Chao

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GAO Jing-tian,HU Peng,CAI Yi-ting等. Performance of pollutant removal and responses of microbial community to Nano-ZnO stress in SBBR[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(8): 3658-3665.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I8/3658

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