紫外耦合游离亚硝酸强化剩余污泥厌氧发酵产酸研究

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Abstract In order to break the bottleneck of the limited hydrolysis performance and low short chain fatty acids (SCFAs) production efficiency from waste activated sludge (WAS) during the traditional anaerobic fermentation, this work investigated the effect of the ultraviolet (UV) assisted free nitrous acid (FNA) pretreatment on WAS disintegration and acidification, and compared with thermal (H) and ultrasonic (US) coupled with FNA pretreatment. Results revealed that UV assisted FNA co-pretreatment (FNA-UV) had a synergistic effect on disruption of both extracellular polymeric substances and cell envelope.･OH and･O₂^-, as the main reaction intermediates, their intensities in FNA-UV group were much stronger than that obtained in other pretreatment groups (FNA-US and FNA-H). Moreover, these two free radicals, with the intermediates such as ･NO,･NO₂ and ONOO^-, further promoting the release of soluble organics. The contents of soluble carbohydrates and protein were 60% and 90% higher than that obtained in FNA group respectively, which served more soluble substrates for SCFAs generation. Accordingly, SCFAs concentration peaked at 4d in FNA-UV group (201.8±4.8)mg COD/g VSS with 56.8% acetic acid (HAc)), which was 67% higher than that of FNA group. Carbon balance analysis at the final stage of the fermentation showed that UV assisted FNA pretreatment played an important role in sludge reduction, release and transformation of soluble substrates, and finally SCFAs production. The functional microbial consortia analysis indicated the anaerobic fermentation bacteria and nitrate-reducing bacteria were obviously enriched in FNA-UV group, which were 23.7%~270.6% higher than that obtained in other groups.

Key words： free nitrous acid (FNA) waste activated sludge (WAS) short chain fatty acids (SCFAs) anaerobic fermentation physical method high-throughput sequencing

Received: 28 December 2021

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X703.1

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	YIN Xiao-yun
	LIU Zhi-hong
	ZHOU Ai-juan
	LI Ya-nan
	YUE Xiu-ping
	CUI Zhi-xuan

Cite this article:

YIN Xiao-yun,LIU Zhi-hong,ZHOU Ai-juan等. Enhancement of acidification from waste activated sludge via anaerobic fermentation by free nitrous acid (FNA) pretreatment assisted by ultraviolet[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(8): 3770-3779.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I8/3770

[1]	Bao H X, Yang H, Zhang H, et al. Improving methane productivity of waste activated sludge by ultrasound and alkali pretreatment in microbial electrolysis cell and anaerobic digestion coupled system[J]. Environmental Research, 2020,180:108863.
[2]	Liu Z H, Zhou A J, Zhang J G, et al. Hydrogen recovery from waste activated sludge:Role of free nitrous acid in a prefermentation-microbial electrolysis cells system[J]. ACS Sustainable Chemistry & Engineering, 2018,6(3):3870-3878.
[3]	Zhou A J, Liu H Y, Varrone C, et al. New insight into waste activated sludge acetogenesis triggered by coupling sulfite/ferrate oxidation with sulfate reduction-mediated syntrophic consortia[J]. Chemical Engineering Journal, 2020,400:125885.
[4]	Zurzolo F, Yuan Q, Oleszkiewicz J. Increase of soluble phosphorus and volatile fatty acids during Co-fermentation of wastewater sludge[J]. Waste and Biomass Valorization, 2016,7(2):317-324.
[5]	Liu X R, Du M T, Yang J N, et al. Sulfite serving as a pretreatment method for alkaline fermentation to enhance short-chain fatty acid production from waste activated sludge[J]. Chemical Engineering Journal, 2020,385,123991.
[6]	金宝丹,王淑莹,邢立群,等.不同发酵方式对污泥厌氧发酵性能的影响及其发酵液利用[J]. 中国环境科学, 2016,36(7):2079-2089. Jin B D, Wang S Y, Xing L Q, et al. The effect of different fermentation methods on the sludge anaerobic fermentation performance and the utilization of fermentation liquor[J]. China Environmental Science, 2016,(36)7:2079-2089.
[7]	Law Y Y, Ye L, Wang Q L, et al. Producing free nitrous acid-A green and renewable biocidal agent-From anaerobic digester liquor[J]. Chemical Engineering Journal, 2015,259:62-69.
[8]	Liu Z H, Zhou A J, Liu H Y, et al. Extracellular polymeric substance decomposition linked to hydrogen recovery from waste activated sludge:Role of peracetic acid and free nitrous acid co-pretreatment in a prefermentation-bioelectrolysis cascading system[J]. Water Research, 2020,176:115724.
[9]	Li X M, Zhao J W, Wang D B, et al. An efficient and green pretreatment to stimulate short-chain fatty acids production from waste activated sludge anaerobic fermentation using free nitrous acid[J]. Chemosphere, 2016,144:160-167.
[10]	Wang Q L, Ye L, Jiang G M, et al. Free nitrous acid (FNA)-based pretreatment enhances methane production from waste activated sludge[J]. Environmental Science and Technology, 2013,47(20):11897-11904.
[11]	Chislett M, Guo J H, Bond P L, et al. Structural changes in model compounds of sludge extracellular polymeric substances caused by exposure to free nitrous acid[J]. Water Research, 2021,188:116553.
[12]	Wang J S, Zhang Z J, Ye X, et al. Enhanced solubilization and biochemical methane potential of waste activated sludge by combined free nitrous acid and potassium ferrate pretreatment[J]. Bioresource Technology, 2020,297:122376.
[13]	Karimi R, Hallaji S M, Siami S, et al. Synergy of combined free nitrous acid and Fenton technology in enhancing anaerobic digestion of actual sewage waste activated sludge[J]. Scientific Reports, 2020,10(1):5027.
[14]	Wu Y Q, Song K, Sun X Y, et al. Mechanisms of free nitrous acid and freezing co-pretreatment enhancing short-chain fatty acids production from waste activated sludge anaerobic fermentation[J]. Chemosphere, 2019,230:536-543.
[15]	Wang Q L, Jiang G M, Ye L, et al. Enhancing methane production from waste activated sludge using combined free nitrous acid and heat pre-treatment[J]. Water Research, 2014,63:71-80.
[16]	Niu Q Q, Xu Q X, Wang Y L, et al. Enhanced hydrogen accumulation from waste activated sludge by combining ultrasonic and free nitrous acid pretreatment:Performance, mechanism, and implication[J]. Bioresource Technology, 2019,285:121363.
[17]	Zhang T T, Wang Q L, Ye L, et al. Combined free nitrous acid and hydrogen peroxide pre-treatment of waste activated sludge enhances methane production via organic molecule breakdown[J]. Scientific Reports, 2015,5:16631.
[18]	Wu Q L, Guo W Q, Bao X, et al. Enhanced volatile fatty acid production from excess sludge by combined free nitrous acid and rhamnolipid treatment[J]. Bioresource Technology, 2017,224:727-732.
[19]	张旭光,陈宇,张龙.游离亚硝酸偶联生物表面活性剂强化污泥厌氧发酵产酸[J]. 环境工程, 2019,37(1):56-60,87. Zhang X G, Chen Y, Zhang L. Enhancement of acid production from sludge anaerobic fermentation by Free nitrous bio-surfactant[J]. Environmental Engineering, 2019,37(1):56-60,87.
[20]	Erwan C, Jean P, Vincent J, et al. Impact of suspended particles on UV disinfection of activated-sludge effluent with the aim of reclamation[J]. Journal of Water Process Engineering, 2018,22:87-93.
[21]	Zheng M, Ping Q, Wang L, et al. Pretreatment using UV combined with CaO2 for the anaerobic digestion of waste activated sludge:Mechanistic modeling for attenuation of trace organic contaminants[J]. Journal of Hazardous Materials, 2021,402:123484.
[22]	Zhang Y P, Li T T, Tian J Y, et al. Enhanced dewaterability of waste activated sludge by UV assisted ZVI-PDS oxidation[J]. Journal of Environmental Sciences, 2022,13(3):152-164.
[23]	Sun J, Guo L, Li Q Q, et al. Structural and functional properties of organic matters in extracellular polymeric substances (EPS) and dissolved organic matters (DOM) after heat pretreatment with waste sludge[J]. Bioresource Technology, 2016,219:614-623.
[24]	Yu H W, Anumol T, Park M, et al. On-line sensor monitoring for chemical contaminant attenuation during UV/H2O2 advanced oxidation process[J]. Water Research, 2015,81:250-260.
[25]	Zhang Y P, Li T T, Tian J Y, et al. Enhanced dewaterability of waste activated sludge by UV assisted ZVI-PDS oxidation[J]. Journal of Environmental Sciences, 2022,113:152-164.
[26]	杨春雪.嗜热菌强化剩余污泥水解及短链脂肪酸积累规律研究[D]. 哈尔滨:哈尔滨工业大学, 2015. Yang C X. Enhanced effects of thermophiles on waste activated sludge hydrolysis and short-chain fatty acids production[D]. Harbin:Harbin Institute of Technology, 2015.
[27]	Donlan R M, Costerton J W. Costerton. Biofilms:survival mechanisms of clinically relevant microorganisms[J]. Clinical Microbiology Reviews, 2002,15(2):167-193.
[28]	Jan T W, Adav S S, Lee D J, et al. Hydrogen fermentation and methane production from sludge with pretreatments[J]. Energy Fuels, 2008, 22(1):98-102.
[29]	Wu Y Q, Song K, Sun X Y, et al. Effects of free nitrous acid and freezing co-pretreatment on sludge short-chain fatty acids production and dewaterability[J]. Science of the Total Environment, 2019,669:600-607.
[30]	Gao Y Q, Peng Y Z, Zhang J Y, et al. Biological sludge reduction and enhanced nutrient removal in a pilot-scale system with 2-step sludge alkaline fermentation and A2O process[J]. Bioresource Technology, 2011,102(5):4091-4097.
[31]	He Z W, Tang C C, Liu W Z, et al. Enhanced short-chain fatty acids production from waste activated sludge with alkaline followed by potassium ferrate treatment[J]. Bioresource Technology, 2019,289:121642.
[32]	Pijuan M, Wang Q L, Liu Y, et al. Improving secondary sludge biodegradability using free nitrous acid treatment[J]. Bioresource Technology, 2012,116:92-98.
[33]	樊雅欣,刘红燕,潘凌峰,等.活化方式对过硫酸盐强化剩余污泥发酵的影响[J]. 中国环境科学, 2019,39:2460-2466. Fan Y X, Liu H Y, Pan L F, et al. Enhancement of waste activated sludge acidification by persulfate:Role of activation methods[J]. China Environmental Science, 2019,39:2460-2466.
[34]	冯宇杰,魏瑶丽,李虹瑶,等.醋糟与剩余污泥共发酵体系中底物配比对挥发性脂肪酸产量的影响[J]. 科学技术与工程, 2020,20(34):14332-14306. Feng Y J, Wei Y L, Li H Y, et al. Effect of Substrate Ratio on the Yield of Volatile Fatty Acids in the Cofermentation System of Vinegar Dregs and Residual Sludge[J]. Science Technology and Engineering, 2020,20(34):14332-14306.
[35]	刘芝宏,魏瑶丽,樊雅欣,等.游离亚硝酸预处理对剩余污泥电解及微生物群落结构的影响[J]. 中国环境科学, 2019,39(7):2953-2959. Liu Z H, Wei Y L, Fan Y X, et al. Role of free nitrous acid on waste activated sludge bio-electrolysis and key microflora shift[J]. China Environmental Science, 2019,39(7):2953-2959.
[36]	张强,李咏梅.投加Na2S对化学强化除磷污泥厌氧发酵释磷的影响[J]. 中国环境科学, 2021,41(3):1219-1227. Zhang Q, Li Y M. Effects of dosing sodium sulfide on phosphorus release during the anaerobic fermentation of waste sludge produced by chemical enhanced phosphorus removal[J]. China Environmental Science, 2021,41(3):1219-1227.
[37]	康晓荣.超声联合碱促进剩余污泥水解酸化及产物研究[D]. 哈尔滨:哈尔滨工业大学, 2013. Kang X R. Study on hydrolysis and acidification of activated sludge enhanced by ultrasound combined with alkaline[D]. Harbin:Harbin Institute of Technology, 2013.
[38]	Donachie S P, Bowman J P, Stephen L W, et al. Arcobacter halophilus sp. nov., the first obligate halophile in the genus Arcobacter[J]. International Journal of Systematic and Evolutionary Microbiology, 2005,55:1271-1277.
[39]	Gerritsen J, Fuentes S, Grievink W, et al. Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat, and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov[J]. International Journal of Systematic and Evolutionary Microbiology, 2014,64(Pt 5):1600-1616.
[40]	贺张伟.预处理方法对污泥厌氧耦合微生物电解及厌氧消化产能的影响[D]. 哈尔滨:哈尔滨工程大学, 2014. He Z W. Study on influence factor and mechanisms of organic matters conversion and phosphorus release during waste activated sludge anaerobic fermentation process[D]. Harbin:Harbin Engineering University, 2014.
[41]	Mielczarek A T, Kragelund C, Eriksen P S, et al. Population dynamics of filamentous bacteria in Danish wastewater treatment plants with nutrient removal[J]. Water Research, 2012,46(12):3781-3795.
[42]	He Q C, Feng C P, Chen N, et al. Characterizations of dissolved organic matter and bacterial community structures in rice washing drainage (RWD)-based synthetic groundwater denitrification[J]. Chemosphere, 2019,215:142-152.
[43]	Simona Č, Jakub K, Tomáš J. Biological water denitrification-A review[J]. Enzyme and Microbial Technology, 1992,14(3):170-183.
[44]	Pervin H M, Batstone D J, Bond P L. Previously unclassified bacteria dominate during thermophilic and mesophilic anaerobic pre-treatment of primary sludge[J]. Systematic and Applied Microbiology, 2013, 36(4):281-290.
[45]	Wang J, Lou Y, Feng K, et al. Enhancing the decomposition of extracellular polymeric substances and the recovery of short-chain fatty acids from waste activated sludge:Analysis of the performance and mechanism of co-treatment by free nitrous acid and calcium peroxide[J]. J Hazard Mater, 2022,423(Pt A):127022.
[46]	Chislett M, Guo J H, Bond P L, et al. Structural changes in cell-wall and cell-membrane organic materials following exposure to free nitrous acid[J]. Environmental Science and Technology, 2020,54(16):10301-10312.
[47]	Wang J S, Zhang Z J, Ye X, et al. Performance and mechanism of free nitrous acid on the solubilization of waste activated sludge[J]. RSC Advances, 2018,8(29):15897-15905.
[48]	Sang S Y, Coakley R, Lau G W, et al. Anaerobic killing of mucoid Pseudomonas aeruginosa by acidified nitrite derivatives under cystic fibrosis airway conditions[J]. Journal of Clinical Investigation, 2006, 116(2):436-446.
[49]	Huang Y, Kong M, Westerman D, et al. Effects of HCO3- on degradation of toxic contaminants of emerging concern by UV/NO3(.)[J]. Environmental Science and Technology, 2018,52(21):12697-12707.
[50]	徐慧敏,秦卫华,何国富,等.超声联合热碱技术促进剩余污泥破解的参数优化[J]. 中国环境科学, 2017,37(9):3431-3436. Xu H M, Qin W H, He G F, et al. Optimization of combined ultrasonic and thermo-chemical pretreatment of waste activated sludge for enhanced disintegration[J]. China Environmental Science, 2017,37(9):3431-3436.
[51]	Thorn K A, Cox L G. Ultraviolet irradiation effects incorporation of nitrate and nitrite nitrogen into aquatic natural organic matter[J]. Journal of Environmental Quality, 2012,41(3):865-881.
[52]	Zheng M, Daniels K D, Park M, et al. Attenuation of pharmaceutically active compounds in aqueous solution by UV/CaO2 process:Influencing factors, degradation mechanism and pathways[J]. Water Research, 2019,164:114922.
[53]	Yang J, Liu X, Wang D, et al. Mechanisms of peroxymonosulfate pretreatment enhancing production of short-chain fatty acids from waste activated sludge[J]. Water Research, 2019,148:239-249.
[54]	Arshad Z, Maqbool T, Shin K H, et al. Using stable isotope probing and fluorescence spectroscopy to examine the roles of substrate and soluble microbial products in extracellular polymeric substance formation in activated sludge process[J]. Science of the Total Environment, 2021,788:147875.
[55]	孙珮石,江映翔,刘安文,等.紫外线辐射对活性污泥除磷性能的增强作用[J]. 中国环境科学, 2003,23(2):184-188. Sun P S, Jiang Y X, Liu A W, et al. Enhancement of phosphorus removal property of activated sludge with UV ray irradiation[J]. China Environmental Science, 2003,23(2):184-188.
[56]	黄芳.高温热水解过程中污泥腐殖酸的演变及其对厌氧消化效果的影响研究[D]. 无锡:江南大学, 2021. Huang F. Evolutions of humic acids during slusge thermal hydrolysis and their effects on anaerobic digestion[D]. Wuxi:Jiangnan University, 2021.
[57]	Luo J Y, Huang W X, Zhang Q, et al. Effects of different hypochlorite types on the waste activated sludge fermentation from the perspectives of volatile fatty acids production, microbial community and activity, and characteristics of fermented sludge[J]. Bioresource Technology, 2020,307:123227.
[58]	Zahedi S, Icaran P, Yuan Z, et al. Effect of free nitrous acid pre-treatment on primary sludge at low exposure times[J]. Bioresource Technology, 2017,228:272-278.
[59]	韩兴.污水紫外消毒装置设计及工艺参数优化研究[D]. 长春:吉林农业大学, 2006. Han X. Research on the design of sewage ultraviolet disinfection equipment and optimization of the craft parameter[D]. Changchun:Jilin Agricultural University. 2006.