热反硝化地芽孢杆菌水解催化剩余污泥提取蛋白质

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Abstract For optimizing the processing conditions to realizing the harmless treatment and resource utilization of excess sludge, the thermophilic bacteria (Bacillus terrestris), which can produce extracellular enzymes such as protease and amylase, were selected from the high-temperature compost substrate and used to extract sludge nitrogen source in this study. Then effects of reaction time, sludge solid content and inoculum amount of crude enzyme solution of thermophilic bacteria on protein extraction were evaluated to determine the optimum reaction conditions. The results show that effects of various factors on protein recovery during hydrolysis were in the order of inoculation amount > time> solid content; and the optimal processing conditions included pH=7, T=60℃, t=8h, and solid content of 4% under which we observed thermophilic bacteria inoculation rate of 20%, protein extraction rate of 32.05%, amino acid content of 359.6mg/L, and polypeptide content of 1060.1mg/L, and that the sludge supernatant particle size decreased gradually with the reaction.

Key words： thermophilic bacteria hydrolysis surplus sludge protein influencing factors

Received: 25 January 2022

PACS:

X703

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	Articles by authors
	DING Ya-ting
	ZHONG Wei-zhang
	QIN Xue
	NING Zhi-fang
	LI Yue
	CHEN Sai-nan

Cite this article:

DING Ya-ting,ZHONG Wei-zhang,QIN Xue等. The extraction of protein from surplus sludge catalyzed by thermophilic Bacillus terrestris hydrolysis[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(8): 3780-3787.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I8/3780

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