石油污染土壤富集前后细菌群落组成和共现网络分析

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Abstract Enrichment culture is one of the most important aspects of isolating petroleum degrading microorganisms, in which the microbial community undergoes corresponding succession. The bacterial community structure, co-occurrence network and core genus composition before and after the enrichment were conducted with the streak plate method, colony PCR and Illumina-MiSeq high-throughput sequencing technology to investigate the succession pattern of bacterial communities in oil-contaminated soil during the enrichment process. The microorganisms in the enriched system were isolated and identified. We observed that the culturable microorganisms in the enriched system belong to 34 genera and 53 species, among which 3 are of potential new species, and Dietzia maris OS33 and Rhodococcus qingshengii OS62-1are able to degrade oil. High-throughput sequencing results show that Proteobacteria and Actinobacteria are the main phylum, whenever it is before or after enrichment. But after enrichment, these two phylums are of absolutely dominant groups and the abundance is up to 97.98%. At the genus level, the dominant genuses are transformed from Psuedomonas, Rhodococcus,Bacillus and Xanthomonas to Dietzia, Unspecified_Idiomarianceae and Halomonas, the core genera are altered to Dietzia for degrating petroleum. The co-occurrence networks of bacterial communities are more simplified and stable after enrichment. The core microorganisms are altered to Pseudomonas, Lysinibacillus, Pseudochrobactrum, Agrobacterium and Lactobacillus which are related to petroleum degradation. The non-petroleum degrading bacteria P. songnenensis P35can cooperate with the oil degrading bacteria D. maris OS33to degrade petroleum.

Key words： petroleum pollution enrichment cultivation co-occurrence networks Dietzia soil high-throughput sequencing community structure

Received: 04 January 2022

PACS:	X53
	Q93

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	Articles by authors
	YU Tian-fei
	LIU Xiao-dong
	AI Jia-min
	WANG Jia-min
	GUO Yi-dan
	LIU Xin-hui
	JIANG Ying-ying
	DENG Zhen-shan

Cite this article:

YU Tian-fei,LIU Xiao-dong,AI Jia-min等. Bacterial community composition and co-occurrence network before and after enrichment of oil-contaminated soil[J]. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(8): 3858-3866.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2022/V42/I8/3858

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